Dimethylsulfoniopropionate in corals and its interrelations with bacterial assemblages in coral surface mucus

Corals produce copious amounts of dimethylsulfoniopropionate (DMSP), a sulfur compound thought toplay a role in structuring coral-associated bacterial communities. We tested the hypothesis that a linkage exists betweenDMSP availability in coral tissues and the community dynamics of bacteria in coral surface mucus. We determinedDMSP concentrations in three coral species (Meandrina meandrites, Porites astreoides and Siderastrea siderea) at twosampling depths (5 and 25 m) and times of day (dawn and noon) at Curac¸ao, Southern Caribbean. DMSP concentration(4–409 nmol cm?2 coral surface) varied with host species-specific traits such as Symbiodinium cell abundance, but notwith depth or time of sampling. Exposure of corals to air caused a doubling of their DMSPconcentration. The phylogeneticaffiliation of mucus-associated bacteria was examined by clone libraries targeting three main subclades of the bacterialDMSP demethylase gene (dmdA). dmdA gene abundance was determined by quantitative Polymerase Chain Reaction(qPCR) against a reference housekeeping gene (recA). Overall, a higher availability of DMSP corresponded to a lowerrelative abundance of the dmdA gene, but this pattern was not uniform across all host species or bacterial dmdA subclades,suggesting the existence of distinct DMSP microbial niches or varying dmdA DMSP affinities. This is the first studyquantifying dmdA gene abundance in corals and linking related changes in the community dynamics of DMSP-degradingbacteria to DMSP availability. Our study suggests that DMSP mediates the regulation of microbe

Theatrical dialogue in teaching the classics

This article addresses some fundamental affinities between theatre and teaching and is based on emerging work in a long-term experiment which we began in the conference ‘Weber/Simmel Antagonisms: Staged Dialogues’, held at the University of Edinburgh on December 2015. Aimed at exploring the possibilities of the theatrical and dialogical forms for teaching the classics of social and cultural theory, it is a risky experiment whose initial results are presented in this special issue. In order to introduce the dialogues and situate them in the context of the broader project, the article does three things: first, it expounds the process of subjectivation at work in both theatre and teaching and explores some of the modalities of the subjective shift sought for in spectators and students. Second, it explains the specificity of this experiment by contrasting it with other uses of theatrical dialogue in teaching. Finally, before briefly introducing each of the dialogues, the article clarifies the fundamental difference between the dialogical form and debate, as radically separating them is at the heart of any experiment in subjectivation, away from the stirring of opinions

Social theory and the politics of big data and method

This article is an intervention in the debate on big data. It seeks to show, firstly, that behind the wager to make sociology more relevant to the digital there lies a coherent if essentially unstated vision and a whole stance which are more a symptom of the current world than a resolute endeavour to think that world through; hence the conclusion that the perspective prevailing in the debate lacks both the theoretical grip and the practical impulse to initiate a much needed renewal of social theory and sociology. Secondly, and more importantly, the article expounds an alternative view and shows by thus doing that other possibilities of engaging the digital can be pursued. The article is thus an invitation to widen the debate on big data and the digital and a call for a more combative social theory

Mineralización del fósforo orgánico

p.157-163Con la finalidad de estudiar el índice de mineralización del fósforo se efectuaron incubaciones de suelos naturales y previamente esterilizados, solos y en contacto con resina Amberlite IRA 400 en su forma C 0 3 H - y Cl- llevada a pH 7,0. Las variaciones del fósforo inorgánico se estudiaron en períodos entre 1 y 56 días

Estatura alta e hipodesenvolvimento mamário após reposição estrogênica em paciente com hipogonadismo hipergonadotrófico e cariótipo 45,X/46,X, der(X) com superdosagem do gene SHOX

SHOX is exclusively expressed in the developing distal limb bones of human embryos and in the first and second pharyngeal arches. It works as a promoter for linear growth and as a repressor of growth plate fusion. It was reported, recently, that SHOX overdosage and gonadal estrogen deficiency have led to tall stature due to continued growth. We report, in the present study, a female patient with 45,X/46,X, psu idic(X)(pter→q21:q21→pter) karyotype, tall stature, and hypergonadotrophic hypogonadism without Turner stigmas. She did not present breast development even after long term therapy with high estrogen doses. Fluorescence in situ hybridization depicted the presence of three copies of SHOX gene. Microsatellite studies showed paternal origin of der(X). Further studies in similarly affected patients will clarify if the absence of breast development, despite previous high-dose estrogen treatment, is associated to triple copy of SHOX gene.O gene SHOX é expresso, exclusivamente, no primeiro e no segundo arcos faríngeos, assim como nas extremidades dos ossos dos membros em embriões humanos. SHOX normalmente atua como um promotor para o crescimento linear e como um repressor do fechamento da placa de crescimento. Recentemente, foi descrito que o excesso da proteína SHOX associada à deficiência estrogênica gonadal leva à estatura alta devido ao contínuo crescimento. Neste estudo descrevemos uma paciente do sexo feminino com cariótipo 45,X/46,X,psu idic(X)(pter→q21:q21→pter), estatura alta, hipogonadismo hipergonadotrófico e sem estigmas de Turner. A paciente não apresentou desenvolvimento de mamas, mesmo depois do tratamento prolongado com altas doses de estrógenos. FISH evidenciou a presença de três cópias do SHOX. Estudo de microssatélites demonstrou a origem paterna do der(X). Estudos futuros em pacientes com semelhanças clínicas esclarecerão se a ausência de desenvolvimento de mamas, apesar do tratamento com altas doses de estrógenos, está associada à tripla cópia do SHOX.Conselho Nacional de Pesquisa (CNPq

Wide spectrum of NR5A1-related phenotypes in 46,XY and 46,XX individuals

Steroidogenic factor 1 (NR5A1, SF-1, Ad4BP) is a transcriptional regulator of genes involved in adrenal and gonadal development and function. Mutations in NR5A1 have been among the most frequently identified genetic causes of gonadal development disorders and are associated with a wide phenotypic spectrum. In 46,XY individuals, NR5A1-related phenotypes may range from disorders of sex development (DSD) to oligo/azoospermia, and in 46,XX individuals, from 46,XX ovotesticular and testicular DSD to primary ovarian insufficiency (POI). The most common 46,XY phenotype is atypical or female external genitalia with clitoromegaly, palpable gonads, and absence of Müllerian derivatives. Notably, an undervirilized external genitalia is frequently seen at birth, while spontaneous virilization may occur later, at puberty. In 46,XX individuals, NR5A1 mutations are a rare genetic cause of POI, manifesting as primary or secondary amenorrhea, infertility, hypoestrogenism, and elevated gonadotropin levels. Mothers and sisters of 46,XY DSD patients carrying heterozygous NR5A1 mutations may develop POI, and therefore require appropriate counseling. Moreover, the recurrent heterozygous p.Arg92Trp NR5A1 mutation is associated with variable degrees of testis development in 46,XX patients. A clear genotype-phenotype correlation is not seen in patients bearing NR5A1 mutations, suggesting that genetic modifiers, such as pathogenic variants in other testis/ovarian-determining genes, may contribute to the phenotypic expression. Here, we review the published literature on NR5A1-related disease, and discuss our findings at a single tertiary center in Brazil, including ten novel NR5A1 mutations identified in 46,XY DSD patients. The ever-expanding phenotypic range associated with NR5A1 variants in XY and XX individuals confirms its pivotal role in reproductive biology, and should alert clinicians to the possibility of NR5A1 defects in a variety of phenotypes presenting with gonadal dysfunction

Design of body adornments obtained from the conformation through a mold and subsequent simple machining of epoxy resin mosaics loaded with fired faience residues

No presente trabalho pretende-se a partir do processo de design avaliar a possibilidade de reutilização e valorização dos resíduos de cacos cerâmicos cozidos da fábrica Bordallo Pinheiro. Para a criação de novos produtos, adornos corporais, tal como anéis, colares, brincos e ganchos. Utilizou-se um processo por moldação via liquída com resina epóxi carregada com resíduos cozidos de faiança que posteriormente foram sujeitos a operações simples de maquinação que permitiram transformar com sucesso os resíduos cerâmicos cozidos em adornos corporais que são compatíveis com mercados de alto valor acrescentado como é no caso do design ou da indústria da moda. Os produtos são da autoria da designer Inês Pereira Coutinho e o trabalho decorreu sob a orientação do professor José Frade.In the present work, it is intended, from the design process, to evaluate the possibilities of reusing and revaluing the wasted shards of fired ceramics from the Bordallo Pinheiro, ceramic factory. Through the creation of new products, ranging within the context of body adornments from rings to hair clips, including necklaces and earrings. Using the process molding with liquid epoxy resin loaded with crushed cooked faience residues then after curing, machine through simple operations, the body adornments came successfully to existence. From mere crushed fired faience to body adornments compatible with high added value markets like the industries of design and fashion. The products are authored by the designer Inês Pereira Coutinho and the work was carried out under the guidance of Professor José Frade