5,750 research outputs found
Banding pattern indicative of echinococcosis in a commercial cysticercosis western blot
<p>Abstract</p> <p>Objective</p> <p>A commercial cysticercosis Western blot was evaluated for serological cross-reactivity of sera from patients with alveolar (AE) and cystic echinococcosis (CE).</p> <p>Methods</p> <p>A total of 161 sera were examined, including 31 sera from AE-patients, 11 sera from CE-patients, 9 sera from patients with other parasitic diseases and 109 sera from patients with unrelated medical conditions. All AE-and CE-sera were also examined by the echinococcosis Western blot.</p> <p>Results</p> <p>More sera from patients with AE than with CE showed cross-reactivity in the form of ladder-like patterns ("Mikado aspect") and untypical bands at 6-8 kDa (71% and 77.4% versus 27.3% and 45.5%, respectively). In contrast, triplets of bands in the area above 50 kDa and between 24 and 39-42 kDa were more frequent in CE than in AE sera. The fuzzy band at 50-55 kDa typical for cysticercosis was absent in all AE and CE sera.</p> <p>Conclusions</p> <p>Atypical banding patterns in the cysticercosis Western blot should raise the suspicion of a metacestode infection different from Taenia solium, i.e. Echinococcus multilocularis or E. granulosus, especially when the Mikado aspect and an altered 6-8 kDa band is visible in the absence of a fuzzy 50-55 kDa band.</p
Integrated Atom Detector Based on Field Ionization near Carbon Nanotubes
We demonstrate an atom detector based on field ionization and subsequent ion
counting. We make use of field enhancement near tips of carbon nanotubes to
reach extreme electrostatic field values of up to 9x10^9 V/m, which ionize
ground state rubidium atoms. The detector is based on a carpet of multiwall
carbon nanotubes grown on a substrate and used for field ionization, and a
channel electron multiplier used for ion counting. We measure the field
enhancement at the tips of carbon nanotubes by field emission of electrons. We
demonstrate the operation of the field ionization detector by counting atoms
from a thermal beam of a rubidium dispenser source. By measuring the ionization
rate of rubidium as a function of the applied detector voltage we identify the
field ionization distance, which is below a few tens of nanometers in front of
nanotube tips. We deduce from the experimental data that field ionization of
rubidium near nanotube tips takes place on a time scale faster than 10^(-10)s.
This property is particularly interesting for the development of fast atom
detectors suitable for measuring correlations in ultracold quantum gases. We
also describe an application of the detector as partial pressure gauge.Comment: 7 pages, 8 figure
An electrochemical study of a liquid crystal used in information displays
The operational lifetime of liquid crystal displays were investigated. Electrochemical reaction at the electrodes of the display can cause failure after 2000 to 3000 hours of operation. Studies using cyclic voltametry of electrochemical reactions of N (p-methoxybenzilidene p-butylaniline (MBBA), a nematic liquid crystal were made. These studies indicate the presence of a reversible reduction of MBBA at the cathode, and that the reduction product undergoes a further reaction leading to products which are not reversibly oxidized. It is concluded that the degradation of the liquid crystal in displays can be reduced with a suitable frequency of alternating voltage
A generalization of the van-der-Pol oscillator underlies active signal amplification in Drosophila hearing
The antennal hearing organs of the fruit fly Drosophila melanogaster boost their sensitivity by an active mechanical process that, analogous to the cochlear amplifier of vertebrates, resides in the motility of mechanosensory cells. This process nonlinearly improves the sensitivity of hearing and occasionally gives rise to self-sustained oscillations in the absence of sound. Time series analysis of self-sustained oscillations now unveils that the underlying dynamical system is well described by a generalization of the van-der-Pol oscillator. From the dynamic equations, the underlying amplification dynamics can explicitly be derived. According to the model, oscillations emerge from a combination of negative damping, which reflects active amplification, and a nonlinear restoring force that dictates the amplitude of the oscillations. Hence, active amplification in fly hearing seems to rely on the negative damping mechanism initially proposed for the cochlear amplifier of vertebrate
Recent Progress in Vector Vortex Coronagraphy
The optical vortex coronagraph has great potential for enabling high-contrast observations very close to bright stars, and thus for reducing the size of space telescopes needed for exoplanet characterization missions. Here we discuss several recent developments in optical vortex coronagraphy. In particular, we describe multi-stage vortex configurations that allow the use of on-axis telescopes for high-contrast coronagraphy, and also enable the direct measurement of the amplitudes and phases of focal plane speckles. We also briefly describe recent laboratory demonstrations of the optical properties of the dual-stage vortex, and of the broadband performance of single stage vortex masks. Indeed, the demonstrated performance of the vector vortex phase masks already in hand, ≈ 10^(-8), is approximately that needed for an initial coronagraphic mission, such as an exoplanet explorer, aimed at detecting exozodiacal light and jovian exoplanets
Upper Limits On Periodic, Pulsed Radio Emission from the X-Ray Point Source in Cassiopeia A
The Chandra X-ray Observatory recently discovered an X-ray point source near
the center of Cassiopeia A, the youngest known Galactic supernova remnant. We
have conducted a sensitive search for radio pulsations from this source with
the Very Large Array, taking advantage of the high angular resolution of the
array to resolve out the emission from the remnant itself. No convincing
signatures of a dispersed, periodic source or of isolated dispersed pulses were
found, whether for an isolated or a binary source. We derive upper limits of 30
and 1.3 mJy at 327 and 1435 MHz for the phase-averaged pulsed flux density from
this source. The corresponding luminosity limits are lower than those for any
pulsar with age less than 10^4 years. The sensitivities of our search to single
pulses were 25 and 1.0 Jy at 327 and 1435 MHz. For comparison, the Crab pulsar
emits roughly 80 pulses per minute with flux densities greater than 100 Jy at
327 MHz and 8 pulses per minute with flux densities greater than 50 Jy at 1435
MHz. These limits are consistent with the suggestion that the X-ray point
source in Cas A adds to the growing number of neutron stars which are not radio
pulsars.Comment: accepted by ApJ Letter
Purification, characterisation and crystallisation of photosystem II from Thermosynechococcus elongatus cultivated in a new type of photobioreactor
AbstractThe thermophilic cyanobacterium Thermosynechococcus elongatus was cultivated under controlled growth conditions using a new type of photobioreactor, allowing us to optimise growth conditions and the biomass yield. A fast large-scale purification method for monomeric and dimeric photosystem II (PSII) solubilized from thylakoid membranes of this cyanobacterium was developed using fast protein liquid chromatography (FPLC). The obtained PSII core complexes (PSIIcc) were analysed for their pigment stoichiometry, photochemical and oxygen evolution activities, as well as lipid and detergent composition. Thirty-six chlorophyll a (Chla), 2 pheophytin a (Pheoa), 9± 1 β-carotene (Car), 2.9±0.8 plastoquinone 9 (PQ9) and 3.8±0.5 Mn were found per active centre. For the monomeric and dimeric PSIIcc, 18 and 20 lipid as well as 145 and 220 detergent molecules were found in the detergent shell, respectively. The monomeric and dimeric complexes showed high oxygen evolution activity with 1/4 O2 released per 37–38 Chla and flash in the best cases. Crystals were obtained from dimeric PSIIcc by a micro-batch method. They diffract synchrotron X-rays to a maximum resolution of 2.9-Å, resulting in complete data sets of 3.2 Å resolution
- …