Role of Dicer Enzyme in the Regulation of Store Operated Calcium Entry (SOCE) in CD4+ T Cells

Background/Aims: Activation of T cell receptors (TCRs) in CD4+ T cells leads to a cascade of signalling reactions including increase of intracellular calcium (Ca2+) levels with subsequent Ca2+ dependent stimulation of gene expression, proliferation, cell motility and cytokine release. The increase of cytosolic Ca2+ results from intracellular Ca2+ release with subsequent activation of store-operated Ca2+ entry (SOCE). Previous studies suggested miRNAs are required for the development and functions of CD4+ T cells. An enzyme called Dicer is required during the process of manufacturing mature miRNAs from the precursor miRNAs. In this study, we explored whether loss of Dicer in CD4+ T cells affects SOCE and thus Ca2+ dependent regulation of cellular functions. Methods: We tested the expression of Orai1 by q-RT-PCR and flow cytometry. Further, we measured SOCE by an inverted phase-contrast microscope with the Incident-light fluorescence illumination system using Fura-2. Intracellular Ca2+ was also measured by flow cytometry using Ca2+ sensitive dye Fluo-4. Results: We found that in Dicer deficient (DicerΔ/Δ) mice Orai1 was downregulated at mRNA and protein level in CD4+ T cells. Further, SOCE was significantly smaller in DicerΔ/Δ CD4+ T cells than in CD4+ T cells isolated from wild-type (Dicerfl/fl) mice. Conclusion: Our data suggest that miRNAs are required for adequate Ca2+ entry into CD4+ T cells and thus triggering of Ca2+ sensitive immune functions

Call Me Caitlyn: Making and making over the 'authentic' transgender body in Anglo-American popular culture

A conception of transgender identity as an ‘authentic’ gendered core ‘trapped’ within a mismatched corporeality, and made tangible through corporeal transformations, has attained unprecedented legibility in contemporary Anglo-American media. Whilst pop-cultural articulations of this discourse have received some scholarly attention, the question of why this 'wrong body' paradigm has solidified as the normative explanation for gender transition within the popular media remains underexplored. This paper argues that this discourse has attained cultural pre-eminence through its convergence with a broader media and commercial zeitgeist, in which corporeal alteration and maintenance are perceived as means of accessing one’s ‘authentic’ self. I analyse the media representations of two transgender celebrities: Caitlyn Jenner and Nadia Almada, alongside the reality TV show TRANSform Me, exploring how these women’s gender transitions have been discursively aligned with a cultural imperative for all women, cisgender or trans, to display their authentic femininity through bodily work. This demonstrates how established tropes of authenticity-via-bodily transformation, have enabled transgender to become culturally legible through the wrong body trope. Problematically, I argue, this process has worked to demarcate ideals of ‘acceptable’ transgender subjectivity: self-sufficient, normatively feminine, and eager to embrace the possibilities for happiness and social integration provided by the commercial domain

A Transiting Planet of a Sun-like Star

A planet transits an 11th magnitude, G1V star in the constellation Corona Borealis. We designate the planet XO-1b, and the star, XO-1, also known as GSC 02041-01657. XO-1 lacks a trigonometric distance; we estimate it to be 200+-20 pc. Of the ten stars currently known to host extrasolar transiting planets, the star XO-1 is the most similar to the Sun in its physical characteristics: its radius is 1.0+-0.08 R_Sun, its mass is 1.0+-0.03 M_Sun, V sini < 3 km/s, and its metallicity [Fe/H] is 0.015+-0.04. The orbital period of the planet XO-1b is 3.941534+-0.000027 days, one of the longer ones known. The planetary mass is 0.90+-0.07 M_Jupiter, which is marginally larger than that of other transiting planets with periods between 3 and 4 days. Both the planetary radius and the inclination are functions of the spectroscopically determined stellar radius. If the stellar radius is 1.0+-0.08 R_Sun, then the planetary radius is 1.30+-0.11 R_Jupiter and the inclination of the orbit is 87.7+-1.2 degrees. We have demonstrated a productive international collaboration between professional and amateur astronomers that was important to distinguishing this planet from many other similar candidates.Comment: 31 pages, 9 figures, accepted for part 1 of Ap

XO-3b: A Massive Planet in an Eccentric Orbit Transiting an F5V Star

We report the discovery of a massive (Mpsini = 13.02 +/- 0.64 Mjup; total mass 13.25 +/- 0.64 Mjup), large (1.95 +/- 0.16 Rjup) planet in a transiting, eccentric orbit (e = 0.260 +/- 0.017) around a 10th magnitude F5V star in the constellation Camelopardalis. We designate the planet XO-3b, and the star XO-3, also known as GSC 03727-01064. The orbital period of XO-3b is 3.1915426 +/- 0.00014 days. XO-3 lacks a trigonometric distance; we estimate its distance to be 260 +/- 23 pc. The radius of XO-3 is 2.13 +/- 0.21 Rsun, its mass is 1.41 +/- 0.08 Msun, its vsini = 18.54 +/- 0.17 km/s, and its metallicity is [Fe/H] = -0.177 +/- 0.027. This system is unusual for a number of reasons. XO-3b is one of the most massive planets discovered around any star for which the orbital period is less than 10 days. The mass is near the deuterium burning limit of 13 Mjup, which is a proposed boundary between planets and brown dwarfs. Although Burrows et al. (2001) propose that formation in a disk or formation in the interstellar medium in a manner similar to stars is a more logical way to differentiate planets and brown dwarfs, our current observations are not adequate to address this distinction. XO-3b is also unusual in that its eccentricity is large given its relatively short orbital period. Both the planetary radius and the inclination are functions of the spectroscopically determined stellar radius. Analysis of the transit light curve of XO-3b suggests that the spectroscopically derived parameters may be over estimated. Though relatively noisy, the light curves favor a smaller radius in order to better match the steepness of the ingress and egress. The light curve fits imply a planetary radius of 1.25 +/- 0.15 Rjup, which would correspond to a mass of 12.03 +/- 0.46 Mjup.Comment: 26 pages, 10 figures. Accepted by ApJ. Current version has several small corrections as a result of a bug in the fitting softwar

XO-2b: Transiting Hot Jupiter in a Metal-rich Common Proper Motion Binary

We report on a V=11.2 early K dwarf, XO-2 (GSC 03413-00005), that hosts a Rp=0.98+0.03/-0.01 Rjup, Mp=0.57+/-0.06 Mjup transiting extrasolar planet, XO-2b, with an orbital period of 2.615857+/-0.000005 days. XO-2 has high metallicity, [Fe/H]=0.45+/-0.02, high proper motion, mu_tot=157 mas/yr, and has a common proper motion stellar companion with 31" separation. The two stars are nearly identical twins, with very similar spectra and apparent magnitudes. Due to the high metallicity, these early K dwarf stars have a mass and radius close to solar, Ms=0.98+/-0.02 Msolar and Rs=0.97+0.02/-0.01 Rsolar. The high proper motion of XO-2 results from an eccentric orbit (Galactic pericenter, Rper<4 kpc) well confined to the Galactic disk (Zmax~100 pc). In addition, the phase space position of XO-2 is near the Hercules dynamical stream, which points to an origin of XO-2 in the metal-rich, inner Thin Disk and subsequent dynamical scattering into the solar neighborhood. We describe an efficient Markov Chain Monte Carlo algorithm for calculating the Bayesian posterior probability of the system parameters from a transit light curve.Comment: 14 pages, 10 Figures, Accepted in ApJ. Negligible changes to XO-2 system properties. Removed Chi^2 light curve analysis section, and simplified MCMC light curve analysis discussio

ANTARES Search for Point Sources of Neutrinos Using Astrophysical Catalogs: A Likelihood Analysis

[EN] A search for astrophysical pointlike neutrino sources using the data collected by the ANTARES detector between 2007 January 29 and 2017 December 31 is presented. A likelihood method is used to assess the signicance of an excess of muon neutrinos inducing track-like events in correlation with the location of a list of possible sources. Different sets of objects are tested in the analysis: (a) a subsample of the Fermi 3LAC catalog of blazars, (b) a jet- obscured population of active galactic nuclei, (c) a sample of hard X-ray selected radio galaxies, (d) a star-forming galaxy catalog, and (e) a public sample of 56 very-high-energy track events from the IceCube experiment. None of the tested sources shows a signi¿cant association with the sample of neutrinos detected by ANTARES. The smallest p-value is obtained for the catalog of radio galaxies with an equal-weights hypothesis, with a pre-trial p- value equivalent to a 2.8sigma excess, which is equivalent to 1.6sigma post-trial. In addition, the results of a dedicated analysis for the blazar MG3 J225517+2409 are also reported: this source is found to be the most signi¿cant within the Fermi 3LAC sample, with five ANTARES events located less than one degree from the source. This blazar showed evidence of ¿aring activity in Fermi data, in spacetime coincidence with a high-energy track detected by IceCube. An a posteriori signicance of 2.6sigma for the combination of ANTARES and IceCube data is reported.The authors acknowledge the financial support of the funding agencies: Centre National de la Recherche Scientifique (CNRS), Commissariat a l'energie atomique et aux energies alternatives (CEA), Commission Europeenne (FEDER fund and Marie Curie Program), Institut Universitaire de France (IUF), LabEx UnivEarthS (ANR-10-LABX-0023 and ANR18-IDEX-0001), Region Ile-de-France (DIM-ACAV), Region Alsace (contrat CPER), Region Provence-Alpes-Cote d'Azur, Departement du Var and Ville de La Seyne-sur-Mer, France; Bundesministerium fur Bildung und Forschung (BMBF), Germany; Istituto Nazionale di Fisica Nucleare (INFN), Italy; Nederlandse organisatie voor Wetenschappelijk Onderzoek (NWO), the Netherlands; Council of the President of the Russian Federation for young scientists and leading scientific schools supporting grants, Russia; Executive Unit for Financing Higher Education, Research, Development and Innovation (UEFISCDI), Romania; Ministerio de Ciencia, Innovacion, Investigacion y Universidades (MCIU): Programa Estatal de Generacion de Conocimiento (refs. PGC2018-096663-B-C41, -A-C42, -B-C43, -B-C44) (MCIU/FEDER), Severo Ochoa Centre of Excellence and MultiDark Consolider (MCIU), Junta de Andalucia (ref. SOMM17/6104/UGR and A-FQM-053UGR18), Generalitat Valenciana: Grisolia (ref. GRISOLIA/2018/119) and GenT (ref. CIDEGENT/2018/034), Spain; Ministry of Higher Education, Scientific Research and Professional Training, Morocco. We also acknowledge the technical support of Ifremer, AIM and Foselev Marine for the sea operation and the CC-IN2P3 for the computing facilities.Albert, A.; Andre, M.; Anghinolfi, M.; Anton, G.; Ardid, M.; Aubert, J.; Aublin, J.... (2021). ANTARES Search for Point Sources of Neutrinos Using Astrophysical Catalogs: A Likelihood Analysis. The Astrophysical Journal. 911(1):1-11. https://doi.org/10.3847/1538-4357/abe53c111911

Constraining the contribution of Gamma-Ray Bursts to the high-energy diffuse neutrino flux with 10 yr of ANTARES data

[EN] Addressing the origin of the astrophysical neutrino flux observed by IceCube is of paramount importance. Gamma-Ray Bursts (GRBs) are among the few astrophysical sources capable of achieving the required energy to contribute to such neutrino flux through p¿ interactions. In this work, ANTARES data have been used to search for upward going muon neutrinos in spatial and temporal coincidence with 784 GRBs occurred from 2007 to 2017. For each GRB, the expected neutrino flux has been calculated in the framework of the internal shock model and the impact of the lack of knowledge on the majority of source redshifts and on other intrinsic parameters of the emission mechanism has been quantified. It is found that the model parameters that set the radial distance where shock collisions occur have the largest impact on neutrino flux expectations. In particular, the bulk Lorentz factor of the source ejecta and the minimum variability time-scale are found to contribute significantly to the GRB-neutrino flux uncertainty. For the selected sources, ANTARES data have been analysed by maximizing the discovery probability of the stacking sample through an extended maximum-likelihood strategy. Since no neutrino event passed the quality cuts set by the optimization procedure, 90 per cent confidence level upper limits (with their uncertainty) on the total expected diffuse neutrino flux have been derived, according to the model. The GRB contribution to the observed diffuse astrophysical neutrino flux around 100 TeV is constrained to be less than 10 per cent.The authors acknowledge the financial support of the funding agencies: Centre National de la Recherche Scientifique (CNRS), Commissariata l'energie atomique et auxenergies alternatives (CEA), Commission Europeenne (FEDER fund and Marie Curie Program), Institut Universitaire de France (IUF), LabEx UnivEarthS (ANR-10-LABX-0023 and ANR-18-IDEX-0001), Region Ile-de-France (DIM-ACAV), Region Alsace (contrat CPER), Region Provence-Alpes-Cote d'Azur, Departement du Var and Ville de La Seyne-sur-Mer, France; Bundesministerium fur Bildung und Forschung (BMBF), Germany; Istituto Nazionale di Fisica Nucleare (INFN), Italy; Nederlandse organisatie voor Wetenschappelijk Onderzoek (NWO), the Netherlands; Council of the President of the Russian Federation for young scientists and leading scientific schools supporting grants, Russia; Executive Unit for Financing Higher Education, Research, Development and Innovation (UEFISCDI), Romania; Ministerio de Ciencia, Innovacion, Investigacion y Universidades (MCIU): Programa Estatal de Generacion de Conocimiento (refs. PGC2018-096663-B-C41, -A-C42, -B-C43, -B-C44) (MCIU/FEDER), Severo Ochoa Centre of Excellence and MultiDark Consolider (MCIU), Junta de Andalucia (ref. SOMM17/6104/UGR and A-FQM-053-UGR18), Generalitat Valenciana: Grisolia (ref. GRISOLIA/2018/119), Spain; Ministry of Higher Education, Scientific Research and Professional Training, Morocco. We also acknowledge the technical support of Ifremer, AIM and Foselev Marine for the sea operation and the CC-IN2P3 for the computing facilities.Albert, A.; Andre, M.; Anghinolfi, M.; Anton, G.; Ardid Ramírez, M.; Aubert, J.; Aublin, J.... (2021). Constraining the contribution of Gamma-Ray Bursts to the high-energy diffuse neutrino flux with 10 yr of ANTARES data. Monthly Notices of the Royal Astronomical Society. 500(4):5614-5628. https://doi.org/10.1093/mnras/staa3503S56145628500

Monte Carlo simulations for the ANTARES underwater neutrino telescope

[EN] Monte Carlo simulations are a unique tool to check the response of a detector and to monitor its performance. For a deep-sea neutrino telescope, the variability of the environmental conditions that can a ect the behaviour of the data acquisition system must be considered, in addition to a reliable description of the active parts of the detector and of the features of physics events, in order to produce a realistic set of simulated events. In this paper, the software tools used to produce neutrino and cosmic ray signatures in the telescope and the strategy developed to represent the time evolution of the natural environment and of the detector e ciency are described.The authors acknowledge the financial support of the funding agencies: Centre National de la Recherche Scientifique (CNRS), Commissariata l'energie atomique et auxenergies alternatives (CEA), Commission Europeenne (FEDER fund and Marie Curie Program), Institut Universitaire de France (IUF), LabEx UnivEarthS (ANR-10-LABX-0023 and ANR18-IDEX-0001), Region Ile-de-France (DIM-ACAV), Region Alsace (contrat CPER), Region Provence-Alpes-Cote d'Azur, Departement du Var and Ville de La Seyne-sur-Mer, France; Bundesministerium fur Bildung und Forschung (BMBF), Germany; Istituto Nazionale di Fisica Nucleare (INFN), Italy; Nederlandse organisatie voor Wetenschappelijk Onderzoek (NWO), the Netherlands; Council of the President of the Russian Federation for young scientists and leading scientific schools supporting grants, Russia; Executive Unit for Financing Higher Education, Research, Development and Innovation (UEFISCDI), Romania; Ministerio de Ciencia e Innovacion (MCI) and Agencia Estatal de Investigacion: Programa Estatal de Generacion de Conocimiento (refs. PGC2018-096663-B-C41, -A-C42, -B-C43, -BC44) (MCI/FEDER), Severo Ochoa Centre of Excellence and MultiDark Consolider, Junta de Andaluca (ref. SOMM17/6104/UGR and A-FQM-053-UGR18), Generalitat Valenciana: Grisola (ref. GRISOLIA/2018/119), Spain; Ministry of Higher Education, Scientific Research and Professional Training, Morocco. We also acknowledge the technical support of Ifremer, AIM and Foselev Marine for the sea operation and the CC-IN2P3 for the computing facilities.Albert, A.; Andre, M.; Anghinolfi, M.; Anton, G.; Ardid Ramírez, M.; Aubert, J.; Aublin, J.... (2021). Monte Carlo simulations for the ANTARES underwater neutrino telescope. JOURNAL OF COSMOLOGY AND ASTROPARTICLE PHYSICS. 2021(1):1-19. https://doi.org/10.1088/1475-7516/2021/01/064S1192021

ANTARES upper limits on the multi-TeV neutrino emission from the GRBs detected by IACTs

[EN] The first gamma-ray burst detections by Imaging Atmospheric Cherenkov Telescopes have been recently announced: GRB 190114C, detected by MAGIC, GRB 180720B and GRB 190829A, observed by H.E.S.S. A dedicated search for neutrinos in space and time coincidence with the gamma-ray emission observed by IACTs has been performed using ANTARES data. The search covers both the prompt and afterglow phases, yielding no neutrinos in coincidence with the three GRBs studied. Upper limits on the energetics of the neutrino emission are inferred. The resulting upper limits are several orders of magnitude above the observed gamma-ray emission, and they do not allow to constrain the available models.The authors acknowledge the financial support of the funding agencies: Centre National de la Recherche Scientifique (CNRS), Commissariat a l'energie atomique et aux energies alternatives (CEA), Commission Europeenne (FEDER fund and Marie Curie Program), Institut Universitaire de France (IUF), LabEx UnivEarthS (ANR-10-LABX-0023 and ANR18-IDEX-0001), Region Ile-de-France (DIM-ACAV), Region Alsace (contrat CPER), Region Provence-Alpes-Cote d'Azur, Departement du Var and Ville de La Seyne-sur-Mer, France; Bundesministerium fur Bildung und Forschung (BMBF), Germany; Istituto Nazionale di Fisica Nucleare (INFN), Italy; Nederlandse organisatie voor Wetenschappelijk Onderzoek (NWO), the Netherlands; Council of the President of the Russian Federation for young scientists and leading scientific schools supporting grants, Russia; Executive Unit for Financing Higher Education, Research, Development and Innovation (UEFISCDI), Romania; Ministerio de Ciencia e Innovacion (MCI) and Agencia Estatal de Investigacion: Programa Estatal de Generacion de Conocimiento (refs. PGC2018-096663-B-C41, -A-C42, -B-C43, -BC44) (MCI/FEDER), Severo Ochoa Centre of Excellence and MultiDark Consolider, Junta de Andalucia (ref. SOMM17/6104/UGR and A-FQM-053-UGR18), Generalitat Valenciana: Grisolia (ref. GRISOLIA/2018/119), Spain; Ministry of Higher Education, Scientific Research and Professional Training, Morocco. We also acknowledge the technical support of Ifremer, AIM and Foselev Marine for the sea operation and the CC-IN2P3 for the computing facilities.Albert, A.; Andre, M.; Anghinolfi, M.; Anton, G.; Ardid Ramírez, M.; Aubert, J.; Aublin, J.... (2021). ANTARES upper limits on the multi-TeV neutrino emission from the GRBs detected by IACTs. JOURNAL OF COSMOLOGY AND ASTROPARTICLE PHYSICS. 2021(3):1-16. https://doi.org/10.1088/1475-7516/2021/03/092S1162021