Prevalence, isolation and identification of bacterial canker pathogens on sweet cherry trees in Tekirdag

2nd International Workshop on Bacterial Diseases of Stone Fruits and Nuts -- APR 21-24, 2015 -- Izmir, TURKEYSweet cherry (Prunus avium L.) is one of the most important fruit trees grown in Tekirdag. Bacterial canker which reduces the yield and quality of sweet cherry fruit and causes death of trees was investigated. For this purpose, a survey study was conducted in 2012-2013, and 129 plant tissue samples were collected from symptomatic trees. As a result of survey studies, bacterial canker was determined in all orchards, and the disease prevalence rate was determined as 17 to 57%. Severity of disease was measured as 20 to 85% in Tekirdag province depending on orchards. Totally, 41 bacterial strains of Pseudomonas syringae were determined by using classical and molecular identification tests, 23 out of 41 yielding. The identity of the 23 strains Pseudomonas syringae pv. morsprunorum and 18 strain Pseudomonas syringae pv. syringae were confirmed by applicans of the expected size 650 bp and 752 bp, respectively.Int Soc Horticultural Sc

Kiraz Dal Yanıklığı Etmenlerinin Tanısı, Fenotipik Ve Genotipik Karakterizasyonu

Kiraz (Prunus avium L.) Tekirdağ’ da yetiştirilen önemli bir meyve türüdür. Kirazda verim ve kaliteyi düşüren, ağaçları kurutan kiraz dal kanseri hastalığı etmen(ler)i bu çalışma ile araştırılmıştır. Tekirdağ ili kiraz bahçelerinde 2012-2013 yıllarında gerçekleştirilen sörvey araştırmaları sonucu 135 hasta bitki örneği belirti gösteren ağaçlardan toplanmıştır. Tekirdağ ilinde sörveylenen bahçelerin tümünde hastalık %20-57 oranında yaygın olduğu, hasta ağaçların %20-85 oranında hastalık şiddetine sahip olduğu tespit edilmiştir. Laboratuarda yapılan izolasyon çalışmalarsonucu bu örneklerden 60 adet Pseudomonas syringae izolatı elde edilmiştir. Yapılan LOPAT testleri sonucunda izolatlar oksidaz, pektolitik aktivite, arginine dehidrolaz negatif, tütünde aşırı duyarlılık ve levan pozitif olarak değerlendirilmiştir. Ayrıca izolatların GATTa özellikleri ise 29 adet izolat (+,+,-,-) özellik gösterirken, 20 adet izolat ise (-,-,+,+) özellik göstermiştir. Tesadüfen seçilen izolat %62-90 oranında benzerlik göstermiştir. Yapılan fenotipik karakterizasyonda izolatlar 2 farklı kümede toplanmaktadır. Moleküler testlerde kullanılan spesifik primerler ile 29 adet izolat 650 bp tekrarlanabilir bant oluşturarak Pseudomonas syringae pv. morsprunorum ve 20 adet izolat 752 bp tekrarlanabilir bant oluşturması nedeniyle Pseudomonas syringae pv. syringae olarak tanılanmıştır. Genotipik olarak izolatlar birbirinden farklı iki kümeye ayrılmıştırSweet cherry (Prunus avium L.) is one of the most important fruit trees grown in Tekirdağ. Causal disease agent(s) of bacterial canker which reduces yield and quality of sweet cherry fruit and cause death of trees were investigated. For this purpose a survey study was conducted in 2012-2013 and 135 infected plant samples were collected from symptomatic trees. As a result of survey studies, bacterial canker was determined in all orchards, while diseases prevalence rate of bacterial canker was determined as 20-57%, severity of disease was measured as 20-85% in surveyed orchards in Tekirdağ Province. As a result of isolation studies, 60 bacterial isolates of Pseudomonas syringae were obtained. Results obtained from LOPAT, oxidase, pectolytic activity and arginine dihydrolase revealed that all isolates were recorded as negative for oxidase, pectolytic activity and arginine dihydrolase, but were positive on tobacco and levan production. Further GATTa characters of tested isolates were found as(+,+,- ,-) for29 isolates, while 20 isolates were recorded as (-,-,+,+). Coincidentally 62-90% of selected isolates showed similarity. All isolates were separated into two clusters based on their phenotypic characterization. By using specific primers, 29 isolates formed 650 bp repeatable band so identified as Pseudomonas syringae pv. morsprunorum, 20 isolates formed 752 bp repeatable band so identified as Pseudomonas syringae pv. syringae. All isolates were separated into two clusters based on their genotypic characterization