NECTAR: Radiography and tomography station using fission neutrons

NECTAR, operated by the Technische Universität München, is a versatile facility for the non-destructive inspection of various objects by means of fission neutron radiography and tomography, respectively

Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains

Cell surface receptors govern a multitude of signalling pathways in multicellular organisms. In plants, prominent examples are the receptor kinases FLS2 and BRI1, which activate immunity and steroid-mediated growth, respectively. Intriguingly, despite inducing distinct signalling outputs, both receptors employ common downstream signalling components, which exist in plasma membrane (PM)-localised protein complexes. An important question is thus how these receptor complexes maintain signalling specificity. Live-cell imaging revealed that FLS2 and BRI1 form PM nanoclusters. Using single-particle tracking we could discriminate both cluster populations and we observed spatiotemporal separation between immune and growth signalling platforms. This finding was confirmed by visualising FLS2 and BRI1 within distinct PM nanodomains marked by specific remorin proteins and differential co-localisation with the cytoskeleton. Our results thus suggest that signalling specificity between these pathways may be explained by the spatial separation of FLS2 and BRI1 with their associated signalling components within dedicated PM nanodomains

The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity

Perception of biotic and abiotic stresses often leads to stomatal closure in plants 1,2. Rapid influx of calcium ions (Ca 2+) across the plasma membrane has an important role in this response, but the identity of the Ca 2+ channels involved has remained elusive 3,4. Here we report that the Arabidopsis thaliana Ca 2+-permeable channel OSCA1.3 controls stomatal closure during immune signalling. OSCA1.3 is rapidly phosphorylated upon perception of pathogen-associated molecular patterns (PAMPs). Biochemical and quantitative phosphoproteomics analyses reveal that the immune receptor-associated cytosolic kinase BIK1 interacts with and phosphorylates the N-terminal cytosolic loop of OSCA1.3 within minutes of treatment with the peptidic PAMP flg22, which is derived from bacterial flagellin. Genetic and electrophysiological data reveal that OSCA1.3 is permeable to Ca 2+, and that BIK1-mediated phosphorylation on its N terminus increases this channel activity. Notably, OSCA1.3 and its phosphorylation by BIK1 are critical for stomatal closure during immune signalling, and OSCA1.3 does not regulate stomatal closure upon perception of abscisic acid—a plant hormone associated with abiotic stresses. This study thus identifies a plant Ca 2+ channel and its activation mechanisms underlying stomatal closure during immune signalling, and suggests specificity in Ca 2+ influx mechanisms in response to different stresses

Performance of the Commercial PP/ZnS:Cu and PP/ZnS:Ag Scintillation Screens for Fast Neutron Imaging

Fast neutron imaging has a great potential as a nondestructive technique for testing large objects. The main factor limiting applications of this technique is detection technology, offering relatively poor spatial resolution of images and low detection efficiency, which results in very long exposure times. Therefore, research on development of scintillators for fast neutron imaging is of high importance. A comparison of the light output, gamma radiation sensitivity and spatial resolution of commercially available scintillator screens composed of PP/ZnS:Cu and PP/ZnS:Ag of different thicknesses are presented. The scintillators were provided by RC Tritec AG company and the test performed at the NECTAR facility located at the FRM II nuclear research reactor. It was shown that light output increases and the spatial resolution decreases with the scintillator thickness. Both compositions of the scintillating material provide similar light output, while the gamma sensitivity of PP/ZnS:Cu is significantly higher as compared to PP/ZnS:Ag-based scintillators. Moreover, we report which factors should be considered when choosing a scintillator and what are the limitations of the investigated types of scintillators