Effects of Proportion of Egg Yolk and Preservation Time on Chilled Semen from Indigenous Rams

The study was set out to determine the effects of different percentages of egg yolk on quality of chilled semen in Indigenous Rams. Different percentages of egg yolk were used to preserve semen from indigenous rams in Tris based extender at 4°C. Nine 2 to 3 years old rams, weighing 21.5±1.2 kg, body condition score 3.9±0.1 with scrotal circumference of 22.4 ±0.4 cm were selected for collection of semen once a week using artificial vagina. Each ejaculate was divided into four portions, and extended with Tris based diluents containing 5, 10, 15, and 20% egg yolk and kept chilled at 4 to 5°C for up to 48h. Motility, viability, functional integrity and morphology were evaluated before and 24h and 48h of preservation. The results showed significantly (p<0.05) better motility, viability and functional integrity with 10% egg yolk compared to other concentrations of egg yolk during preservation. However, the proportion of egg yolk did not affect spermatozoa quality before preservation and normal morphology in any time during preservation. Time of preservation decreased (p<0.01) the rate of motility, viability, functional integrity, and normal morphology of spermatozoa. Positive correlation coefficient observed between spermatozoa motility, viability, and functional integrity. Functional integrity of spermatozoa positively correlated to morphologically normal spermatozoa. It is concluded that 10% egg yolk in Ttris based diluents may be best for chilled Indigenous ram semen

Comparison of Estrus Synchronization by PGF2α and Progestagen Sponge with PMSG in Indigenous Ewes in Bangladesh

The objective of this study was to compare the efficacy of two different synchronizing agents with different doses in indigenous ewes in Bangladesh. Indigenous ewes (n=65) were allocated initially into four groups and treated with intravaginal sponges containing two doses of Flurogestone Acetate (FGA 30 and 45 mg) for 12 days and two doses of PGF2α injection (Cloprostenol 100 and 175 μg) at 9 days apart. Ewes in estrus were identified using vasectomized rams. An investigation was made on vaginal smear in indigenous ewes. Fifty days after estrus and natural mating, pregnancy was determined by trans-cutaneous ultrasonography.. All the indigenous ewes used in four treatment groups exhibited signs of estrus during the observation period. Among sexual behaviors, firm standing, head-turning and soliciting were displayed more frequently. Ewes in Group I and Group II started to express receptive behavior between 48 h to70 h and in Group III and Group IV between 18-36 h following sponge removal. The time to estrus was slightly longer (58.6 ±2.64 h) in the groups treated with 175 μg Cloprostenol compared to the other groups (55.8 ±3.12, 32.9±1.60 and 30.8±2.04 h).There was significant differences (PFGA compared with other range of time. Similarly, 52.9 and 62.5% of ewes in Cloprostenol (100 and175 μg, respectively) showed oestrus during 49 to 60 h. There was no significant difference in ewes of all treatedgroups (P˃0.05). The estrus period was shown the predominance of superficial cells that was keratinized, largely anucleate, and had angular, folded cell margins or with pyknotic nuclei (Figures 5-6) in the vaginal smear of ewes in all the treated groups. The higher pregnancy rates observed in the 30 mg FGA based Group III (100%) compared with 100 μg Cloprostenol (88.2 %), 175 μg Cloprostenol (75%) and 45 mg FGA (93.8 %) group. It was concluded that though FGA sponge protocol presented superior results, PGF2α protocol was as efficient in synchronizing estrus as the former in indigenous ewes in Bangladesh

Baseline Study of Reproductive Performances of Indigenous Rams in Bangladesh

9 , respectively. The rate of motility: 89. 0±0.2, 72.4±0.2, 62.0±0.6, viability: 91.8±0.1, 75.6±0.2, 64.8±0.6, functional integrity: 87.3±0.2, 69.1±0.2, 50.2±0.5 and normal spermatozoa were 94.0±0.1, 77.3±0.1 and 75.0±0.2 in fresh, chilled and frozen semen, respectively

Identification of non-cerebral cyst: Zoonotic Taenia multiceps in domestic goat in Bangladesh

Aim: This study was performed to identify the non-cerebral Taenia multiceps cyst through molecular phylogeny of the 12S rRNA gene. Materials and Methods: Eight cyst samples were collected from 385 examined slaughtered goats during October 2015-September 2016 from three slaughterhouses in Chittagong City Corporation. Cysts were removed from the thigh muscle, and scolices were collected for light microscopic examination and molecular identification. The DNA was extracted and analyzed by polymerase chain reaction using 12S rRNA gene primers. Cyst samples were also preserved in 10% buffered formalin for histopathological study. Results: T. multiceps non-cerebral cyst is 2.1% prevalent in goat in this area. Under light microscopic examination, scolex was found with four suckers and a rostellum with the double crown of 32 hooks and hooklets. Molecularly, all the samples were amplified with 12S rRNA gene fragments yielded 270 base pair amplicon. Zenker's necrosis with focal to diffuse infiltration of lymphocytes and eosinophil was also found around the cyst wall in histopathological examination. Conclusion: Although the non-cerebral form of the cysts produced by T. multiceps is genetically identical with the cerebral cyst, previously published data indicated that cerebral T. multiceps cyst is predominant in other parts of the world as well as in Bangladesh. This study showed that non-cerebral cyst is also prevalent in this country which is very important for public health concern. This study depicts an idea of non-cerebral form of zoonotic T. multiceps cyst which will be helpful in taenia cyst control and prevention