74 research outputs found
Thermoresponsive Micropatterned Substrates for Single Cell Studies
We describe the design of micropatterned surfaces for single cell studies, based on thermoresponsive polymer brushes. We show that brushes made of poly(N-isopropylacrylamide) grafted at high surface density display excellent protein and cell anti-adhesive properties. Such brushes are readily patterned at the micron scale via deep UV photolithography. A proper choice of the adhesive pattern shapes, combined with the temperature-dependent swelling properties of PNIPAM, allow us to use the polymer brush as a microactuator which induces cell detachment when the temperature is reduced below C
SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras
SHANK3, a synaptic scaffold protein and actin regulator, is widely expressed outside of the central nervous system with predominantly unknown function. Solving the structure of the SHANK3 N-terminal region revealed that the SPN domain is an unexpected Ras-association domain with high affinity for GTP-bound Ras and Rap G-proteins. The role of Rap1 in integrin activation is well established but the mechanisms to antagonize it remain largely unknown. Here, we show that SHANK1 and SHANK3 act as integrin activation inhibitors by sequestering active Rap1 and R-Ras via the SPN domain and thus limiting their bioavailability at the plasma membrane. Consistently, SHANK3 silencing triggers increased plasma membrane Rap1 activity, cell spreading, migration and invasion. Autism-related mutations within the SHANK3 SPN domain (R12C and L68P) disrupt G-protein interaction and fail to counteract integrin activation along the Rap1-RIAM-talin axis in cancer cells and neurons. Altogether, we establish SHANKs as critical regulators of G-protein signalling and integrin-dependent processes
Effects of Tailored Surface Chemistry on Desorption Electrospray Ionization Mass Spectrometry: a Surface-Analytical Study by XPS and AFM
ToF-SIMS surface and interface characterization of the immobilized camel antibody (cAb) onto SAMs-COOH/Au substrates
Immobilization of camel antibody (cAb-PSA-N7) onto Au/SAM-COOH substrates:Characterization and quantification by radiolabelling, XPS and ToF-SIMS measurements
Immobilization of camel antibody (cAb-PSA-N7) onto Au/SAM-COOH substrates:Characterization and quantification by radiolabelling, XPS and ToF-SIMS measurements
Preparation, characterisation and biomedical applications of conducting polymer particles:Colloid Polymers: Preparation and Biomedical Applications, H. Elaissari (Ed.), Marcel Dekker Inc. (NY), SURFACTANT SCIENCE SERIES (ISSN: 0081-9603), 2003; ISSU 115; 245-284
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