PRODUCTION OF BIODIESEL FROM SOME VEGETABLE OILS

Biodiesel is becoming prominent among the alternatives to conventional petro-diesel due to economic,environmental and social factors. The quality of biodiesel is influenced by the nature of feedstock and theproduction processes employed. High amounts of free fatty acids (FFA) in the feedstock are known to bedetrimental to the quality of biodiesel. In addition, oils with compounds containing hydroxyl groups possesshigh viscosity due to hydrogen bonding. American Standards and Testing Materials, (ASTM D 6751)recommends FFA content of not more than 0.5% in biodiesel and a viscosity of less than 6 mm2/s. Thephysico-chemical properties of palm kernel oil and coconut oil were assessed for their potentials in biodiesel.The properties of palm kernel oil and coconut oil were compared with those of palm from literature whilethat of biodiesel were compared with petro-diesel, ASTM and European Standards (EN14214). Resultsshowed that high amounts of FFA in oils produced low quality biodiesel while neutralized oils with lowamounts of FFA produced high quality biodiesel. The quality of biodiesel from palm kernel oil and coconutoils was improved greatly by neutralising the crude oil

Comparative Effects of Silver Nanoparticles, Sucrose and Sodium Chloride as Osmotic Solutions for Tomato Slices: Antioxidant Activity, Microbial Quality and Modelling with Polynomial Regression Model

This study has reported comparative effects of silver nanoparticles  (AgNPs),  sucrose and sodium chloride as osmotic solutions on antioxidant activity and microbial quality of 10 mm tomato slices. 40 g of tomato slices were dehydrated osmotically (OD) at different temperatures (60, 70 and 80 °C) and time (30, 60, 90, 120 150 and 180 min).Water loss, solid  impregnation, water and solid diffusivities of tomato slices were found to increase with increase in solution temperatures and concentrations with AgNPs having the greatest influence. Antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl increased with increase in solution concentrations but decreased with increase in temperature. Three-wayANOVA(R2=0.998) revealed additive statistically significant effects of osmotic agents,  concentrations and temperatures on antioxidant activity;   F(8,54)=67.854,P=0.00. Polynomial regression analysis with response surface methodology validated experiments such that for each unit increase in concentration and temperature, antioxidant activity increased with good coefficients of determination; sucrose (R2 = 0.87), NaCl, (R2 = 0.89) andAgNPs (R2 = 0.91). Potato dextrose and nutrient agars were used for isolating and identifying microorganisms in OD tomato slices. Tomato slices dehydrated with AgNPs had the highest microbial inhibition of fungi with growth occurring after 7 days, unlike in treatments with sucrose and NaCl where fungal growth appeared after 2 and 5 days, respectively. Aspergillus niger was the most prevalent fungus. It can be concluded that AgNPs may serve as a viable means to dehydrate and preserve tomatoes without loss of antioxidant activity.Keywords: Osmotic dehydration, polynomial regression, response surface, antioxidant activity, three-way ANOVA, silver nanoparticles

Bacteriological pollution indicators in Ogun River flowing through Abeokuta Metropolis

Water resources are significant part of integrated community development policy and good health. Hence, the need to reduce the impact of natural and anthropogenic pollution causes so as to enhance water quality. The bacteriological quality of the Ogun River was investigated to determine the sanitary conditions of the water body between March and August, 2014. Total heterotrophic bacteria counts (THBC), total coliform counts (TCC) and total Escherichia coli counts (TEC) using standard plate count and Most Probable Number (MPN) techniques were determined. The isolates were identified using 16SrRNA gene. Total heterotrophic bacteria counts varied between 1.13 × 106 and 4.1 × 107 CFU/ml, TCC ranged between 2.5 × 105 and 2.33× 107 CFU/ml and TEC was between 5 × 104 and 1.3 × 106 CFU/ml. Most Probable Number of coliforms in all samples varied between 120 and 1600 MPN/100 ml. Isolated microorganisms include Escherichia coli strain SUS9EC, Escherichia coli O157:H7 strain SSI7, Escherichia coli strain BW25113, Escherichia coli strain C-X1B, and Klebsiella oxytoca strain KU-5. One-wayanalysis of variance showed significant difference within the samples at (P<0.05).The results revealed high bacteria counts which is higher than the recommended value of 1.2 × 102 for THBC, a zero E. coli count and not more than 10 coliforms per 100 ml by World Health Organization standards for drinking water.Keywords: Water, bacteria, Escherichia coli, pollutio

Perceptions and beliefs of community gatekeepers about genomic risk information in African cleft research

BACKGROUND: A fundamental ethical issue in African genomics research is how socio-cultural factors impact perspectives, acceptance, and utility of genomic information, especially in stigmatizing conditions like orofacial clefts (OFCs). Previous research has shown that gatekeepers (e.g., religious, political, family or community leaders) wield considerable influence on the decision-making capabilities of their members, including health issues. Thus, their perspectives can inform the design of engagement strategies and increase exposure to the benefits of genomics testing/research. This is especially important for Africans underrepresented in genomic research. Our study aims to investigate the perspectives of gatekeepers concerning genomic risk information (GRI) in the presence of OFCs in a sub-Saharan African cohort.METHODS: Twenty-five focus group discussions (FGDs) consisting of 214 gatekeepers (religious, community, ethnic leaders, and traditional birth attendants) in Lagos, Nigeria, explored the opinions of participants on genomic risk information (GRI), OFC experience, and the possibility of involvement in collaborative decision-making in Lagos, Nigeria. Transcripts generated from audio recordings were coded and analyzed in NVivo using thematic analysis.RESULTS: Three main themes-knowledge, beliefs, and willingness to act-emerged from exploring the perspective of gatekeepers about GRI in this group. We observed mixed opinions regarding the acceptance of GRI. Many participants believed their role is to guide and support members when they receive results; this is based on the level of trust their members have in them. However, participants felt they would need to be trained by medical experts to do this. Also, religious and cultural beliefs were crucial to determining participants' understanding of OFCs and the acceptance and utilization of GRI.CONCLUSIONS: Incorporating cultural sensitivity into public engagement could help develop appropriate strategies to manage conflicting ideologies surrounding genomic information in African communities. This will allow for more widespread access to the advances in genomics research in underrepresented populations. We also recommend a synergistic relationship between community health specialists/scientists, and community leaders, including spiritual providers to better understand and utilize GRI.</p

Perceptions and beliefs of community gatekeepers about genomic risk information in African cleft research

BACKGROUND: A fundamental ethical issue in African genomics research is how socio-cultural factors impact perspectives, acceptance, and utility of genomic information, especially in stigmatizing conditions like orofacial clefts (OFCs). Previous research has shown that gatekeepers (e.g., religious, political, family or community leaders) wield considerable influence on the decision-making capabilities of their members, including health issues. Thus, their perspectives can inform the design of engagement strategies and increase exposure to the benefits of genomics testing/research. This is especially important for Africans underrepresented in genomic research. Our study aims to investigate the perspectives of gatekeepers concerning genomic risk information (GRI) in the presence of OFCs in a sub-Saharan African cohort.METHODS: Twenty-five focus group discussions (FGDs) consisting of 214 gatekeepers (religious, community, ethnic leaders, and traditional birth attendants) in Lagos, Nigeria, explored the opinions of participants on genomic risk information (GRI), OFC experience, and the possibility of involvement in collaborative decision-making in Lagos, Nigeria. Transcripts generated from audio recordings were coded and analyzed in NVivo using thematic analysis.RESULTS: Three main themes-knowledge, beliefs, and willingness to act-emerged from exploring the perspective of gatekeepers about GRI in this group. We observed mixed opinions regarding the acceptance of GRI. Many participants believed their role is to guide and support members when they receive results; this is based on the level of trust their members have in them. However, participants felt they would need to be trained by medical experts to do this. Also, religious and cultural beliefs were crucial to determining participants' understanding of OFCs and the acceptance and utilization of GRI.CONCLUSIONS: Incorporating cultural sensitivity into public engagement could help develop appropriate strategies to manage conflicting ideologies surrounding genomic information in African communities. This will allow for more widespread access to the advances in genomics research in underrepresented populations. We also recommend a synergistic relationship between community health specialists/scientists, and community leaders, including spiritual providers to better understand and utilize GRI.</p

A case of polydactyly in the hind-limbs of a West African Dwarf goat in South-West Nigeria

This report describes a case of polydactyly in the hind-limb of a West African Dwarf goat kid in South West Africa. Physical examination revealed the presence of four digits in each of the hind limbs. Radiological examination and macerated bones of the animal showed a bifid shape of each metatarsal that was more prominent from the distal half of the diaphysis. This resulted in the presence of four articulating surfaces per limb at the distal extremity. Though this condition is rare in goats, we advise that continuous reporting by researchers can give a better prevalence statistics of these occurrences

Shared genetic risk between major orofacial cleft phenotypes in an African population

Nonsyndromic orofacial clefts (NSOFCs) represent a large proportion (70%–80%) of all OFCs. They can be broadly categorized into nonsyndromic cleft lip with or without cleft palate (NSCL/P) and nonsyndromic cleft palate only (NSCPO). Although NSCL/P and NSCPO are considered etiologically distinct, recent evidence suggests the presence of shared genetic risks. Thus, we investigated the genetic overlap between NSCL/P and NSCPO using African genome-wide association study (GWAS) data on NSOFCs. These data consist of 814 NSCL/P, 205 NSCPO cases, and 2159 unrelated controls. We generated common single-nucleotide variants (SNVs) association summary statistics separately for each phenotype (NSCL/P and NSCPO) under an additive genetic model. Subsequently, we employed the pleiotropic analysis under the composite null (PLACO) method to test for genetic overlap. Our analysis identified two loci with genome-wide significance (rs181737795 [p = 2.58E−08] and rs2221169 [p = 4.5E−08]) and one locus with marginal significance (rs187523265 [p = 5.22E−08]). Using mouse transcriptomics data and information from genetic phenotype databases, we identified MDN1, MAP3k7, KMT2A, ARCN1, and VADC2 as top candidate genes for the associated SNVs. These findings enhance our understanding of genetic variants associated with NSOFCs and identify potential candidate genes for further exploration.</p

GIS based mapping of spatio-temporal distribution pattern of ribbonfish Trichiurus lepturus (Linnaeus, 1758) along Saurashtra coast, India

Identification of spatio-temporal distribution of fish species represents fundamental information for stock assessment which in turn is essential for formulation of fishery management plans as well as for GIS based decision making. This study investigated the spatio-temporal distribution and abundance of different life stages of ribbonfish, Trichiurus lepturus (Linnaeus, 1758) off Saurashtra coast. Geographical coordinates of fishing and allied information on the time of fishing, depth of the fishing area as well as catch details were collected using a structured schedule, from selected trawlers operated from Veraval. Information was mapped using a GIS software to get the spatio-temporal distribution of the species. It was observed that juveniles were more abundant along the south Saurashtra coast whereas subadults and adults showed a discontinuous distribution with abundance in the waters off south Saurashtra as well as north Saurashtra coasts