Ineffectiveness of Methylation in Rgulation of VHL, ECAD, and RUNX3 Genes in Erythroid Cells Differentiated by Erythropoietin

Background: Vast variety of intermediate factors including cell cycle regulators, growth factors, transcription factors, and signaling pathways are involved in hematopoietic stem cell (HSC) commitment and differentiation into distinct lineages. VHL, Ecad, and RUNX3 are among these. Epigenetics is currently introduced as a potential mechanism to control the gene regulation. The aim of this study is to reveal the correlation between the expression level and methylation pattern of mentioned genes after in vitro differentiation of cord blood HSCs into erythroid lineage mediated by erythropoietin.Materials and Methods: After isolation and expansion, the CD34+ cord blood stem cells were divided into two parts. The first part was used to extract the DNA and RNA and the second to differentiate into erythroid lineage. Methylation specific PCR (MSP) and Real-time PCR were used to determine the methylation status and expression levels of the genes, respectively.Results: Although the significant upregulation observed for VHL and Ecad genes and a down-regulation for RUNX3 gene after differentiation, no remarkable changes were seen in methylation pattern compared with cord blood HSCs by MSP technique.Conclusion: It is appearing that methylation pattern in promoter region has not an effective role in expression of VHL, Ecad, and RUNX3. Moreover, considering the inability of MSP method to detect subtle differences in methylation level a more sensitive method is needed to distinguish the methylation levels of these genes before and after erythroid differentiation

Government and SMEs in the Maldives and Mauritius

The purpose of this paper is to compare and contrast the role of government in the development of SMEs in the Maldives and Mauritius. Using tourism SMEs, it seeks to identify, analyse and compare strategies deployed by SMEs operating in an “enabling” and a “constrained” business environment.  An inductive approach to qualitative research is undertaken using seventeen semi-structured interviews with key stakeholders identified though a stakeholder analysis. Using multiple sources (six government officials, eight SME owner/managers, one private bank owner, one academic and one resort owner), variations and consensus in the data were identified through thematic analysis.  The Maldivian Government is less proactive in supporting its SMEs compared to the Mauritian Government. Its failure to facilitate access to finance and provide business support services has led the Maldivian SMEs to use multiple methods of bootstrapping to sustain existing businesses and/or start new ones. In contrast, despite operating in a more enabling business environment, Mauritian SMEs were found to engage in similar strategies due to lack of trust in government-led initiatives. Practical implications – Policy-makers in island economies can use the findings to inform decision making in SME development planning.  While this research adds to the sparse literature on government and SME development in island economies, it also highlights the relevance of bootstrapping for SMEs operating in economically constrained environments

Survey of clinical features, pathogenesis and therapeutic options for Ebola haemorrhagic fever

      The genus Ebola virus first was recognized in 1976, when two outbreaks occurred in Zaire and Sudan. Ebola virus disease (EVD) is a highly contagious disease that can affect both human and nonhuman primates: Zaire ebolavirus (ZEBOV), Sudan ebolavirus (SEBOV), Côte d’Ivoire ebolavirus (CEBOV), Bundibugyo ebolavirus (BEBOV) and Reston ebolavirus (REBOV) are five members of the Filoviridae family that can cause haemorrhagic fever. EVD is transmitted by direct contact with contaminated blood or other biological fluids of the infected animals such as chimpanzees, gorillas, fruit bats, monkeys, forest antelope and porcupines found ill or dead or in the rainforest. Ebola is responsible for different clinical futures that can be ranged from fever, headache, arthralgia, myalgia, abdominal pain, anorexia and vomiting to severe respiratory disorders, viral hemorrhagic fever, cardio-vascular disorders and hypovolaemic shock.  Although there is no specific treatment for EVD, considerable advances like use of monoclonal antibody, intefron and Favipiravir/T-705 as effective chemotherapeutic agent in treatment of EBV have been made. To date, 25 outbreaks of EVD have been reported. Hence, EVD as a zoonotic disease should be more focused not only in endemic area but also in throughout the world. Awareness of the disease and routes of transmission and also continuous surveillance to combat disease and outbreaks is necessary

Autonomous and Connected Vehicles Canadian Market Comprehensive Readiness Assessment

The arrival of the automobile changed the shape of 20th-century cities around the world, now in 21st-century, autonomous vehicles (AVs) and connected vehicles (CVs) will change the shape and culture of the world’s cities once more. AVs and CVs are being deployed all around the world; the reason for this rapid development is that AVs and CVs enrich societies and industries with substantial benefits such as environmental effects, reducing Urbain’s noise and air pollution, safety, mobility for everyone, transportation as-a-service, smart transportation, etc. A market readiness assessment is a way to evaluate and identify that the market is prepared for launching technology and also whether the market will respond positively to that technology or not. The objectives of this research are to enhance Canadian society and policymakers’ knowledge and understanding of AVs and CVs technologies and examine the maturity of the Canadian market for obtaining the most benefits from these technologies. The four main pillars include policy and legislation, technology and innovation, infrastructure, and customer acceptance. A readiness score model was provided to calculate the four main pillars readiness score regarding AVs and CVs technologies state in Canada. The results of this study show that the Canadian government has not taken specific actions to enhance public awareness about AVs and CVs technologies, road quality and EVs charging stations should be considered the main challenges in the infrastructure pillar, the absence of OEMs headquarters, low investment of companies, and absence of R&D cores were obstacles and challenges for the technology and innovation sector regarding AVs and CVs technologies. Keywords: Autonomous Vehicles, Connected Vehicles, Market readiness assessment

Study of flagellin profiling in multidrug resistant Pseudomonas aeruginosa (MDRPA) isolated from burn wound infections, Tehran, Iran

     Nosocomial infections of multidrug-resistant Pseudomonas aeruginosa (MDRPA) are a growing concern in hospitalized patients in burn centers. The aim of this study was to investigate the flagellin profiling and antibiotic susceptibility of P. aeruginosa isolated from burn wound infections. During 8 month study, 73 clinically P. aeruginosa isolates collected from patients hospitalized in burn ward. P. aeruginosa isolates were identified using standard laboratory procedures. In vitro susceptibility of clinical isolates of P. aeruginosa to 6 antimicrobial agents were investigated by Clinical and Laboratory Standards Institute (CLSI 2012) Kirby-Bauer disk diffusion assay. The frequency of different type of flagellin was investigated by using specific primers and by PCR method. The resistance rates of our isolates to 6 tested antimicrobial agents were relatively high. Imipenem has good activity while tobramycin and ciprofloxacin do not have any effect on P. aeruginosa isolates. Of 73 isolates 59 (80.8%) were multidrug resistant. Twenty eight of 73 isolates were resistant to all antibiotics. Agarose gel electrophoresis of chromosomal DNA exhibited that 59 isolates (80.8%) of P. aeruginosa had type A flagellin while only 14 isolates (19.2%) had type b flagellin. Given the antibiotic failure treatment, it appears that alternative ways such as immunity to prevent of these infections could be informative. Our survey of flagellin profiling of multidrug-resistant P. aeruginosa isolates exhibited high frequency of type a flagellin as a major virulence factor has important role of immunity against infections caused by MDRPA. Functional surveillance of multidrug-resistant P. aeruginosa in order to prevention of resistance dissemination is necessary.