Clasificación de pimentones por índices de calidad espectrofotométricos. (Técnicas espectrofotométricas para la determinación de la calidad del pimentón).

Existe la necesidad de disponer de sistemas objetivos y rápidos para la determinación de la calidad de los productos agrícolas. Los sistemas ópticos y espectrofotométricos se han presentado como muy válidos para estos fines en muchos productos (Mohsenin, 1984) (tomates, peras, manzanas, patatas, carnes, huevos...) esto justifica el uso de estos métodos sobre nuevos materiales. Las restricciones impuestas a los colorantes artificiales por las nuevas legislaciones favorecen el consumo de colorantes de origen natural, como el pimentón. Es necesario encontrar un sistema rápido y eficaz que determine la calidad de este producto de creciente interés, (Mínguez, 1992; Conrad, 1987; Nagle 1979; Navarro y Costa, 1993 ). Además existen graves problemas en la comercialización de este producto por la poca transparencia del mercado. Se han producido, en los últimos años, importaciones de pimentón de Marruecos de muy baja calidad y precio, que se mezclaban con pequeñas proporciones de pimentón de humo, comercializándose como pimentón de alta calidad y a precios similares al pimentón producido en la Vera por el sistema tradicional, lo que supone un fraude. Es por tanto necesario encontrar un sistema que discrimine producto de alta calidad ( con color atractivo, alto nivel de carotenoides y alta estabilidad ) de pimentones de baja calidad o dañados ( por exceso de calor durante el secado, enranciamiento, etc.). El objetivo de este trabajo ha sido segregar diversas calidades de pimentón, para esto se disponía de pimentones procedentes de Extremadura, de líneas seleccionadas obtenidas por el SIA, secados por el método tradicional (humo) y con estufa, incluyendo muestras que presentan daños por quemado. Sin ningún conocimiento previo se ha trabajado sobre espectros de reflexión en VIS y NIR a los que se han aplicado ciertas transformaciones para la obtención de variables que fueran capaces de discriminar pimentones secados con humo de los secados con estufa y de los quemados. Realizando reiterados estudios estadísticos se ha llegado a establecer algunas variables útiles en la separación de estas tres clases, para ambos rangos del espectro

Aumento de la fertilidad mediante técnicas de procesamiento avanzado de semen: caso clínico

Current equine reproductive clinic aims to improve subfertile stallions semen quality, (which is furthermore affected by the processing protocol) and maintain this quality during storage. The aim of this paper is to present a case referred to the Hospital Clínico Veterinario Complutense, of a stallion with low sperm quality, and 0% fertility rate in the first batch of mares served. Due to these results it was decided to perform a special semen processing protocol, developed by our laboratory, in order to increase its quality throughout a colloidal centrifugation technique with EquiPure® This system is able to increase semen quality and maintain itduring cooling preservation, obtaining results of around 57,1% of total fertility, which validates this technique as an effective method for subfertile stallions semen processing.La clínica reproductiva equina actual persigue mejorar la calidad seminal de sementales subfértiles, (la cual, además, se ve afectada por el procesamiento del semen) y que ésta se conserve durante el almacenamiento. El objetivo de este trabajo es presentar un caso clínico remitido al Hospital Clínico Veterinario Complutense, sobre un semental que presenta una baja calidad seminal, siendo la fertilidad del primer lote de yeguas del 0%. Ante estos resultados se decide realizar un procesado del semen, desarrollado por nuestro laboratorio, enfocado a aumentar su calidad mediante un protocolo de centrifugación coloidal con EquiPure® (Nidacon International AB). La aplicación de este protocolo consigue aumentar la calidad seminal y mantenerla durante la conservación en refrigeración, obteniéndose unos resultados de fertilidad total del 57,1%, lo que lo valida como método efectivo para el procesado del semen en casos de sementales subfértiles

Towards a clinical staging for bipolar disorder: defining patient subtypes based on functional outcome.

BACKGROUND: The functional outcome of Bipolar Disorder (BD) is highly variable. This variability has been attributed to multiple demographic, clinical and cognitive factors. The critical next step is to identify combinations of predictors that can be used to specify prognostic subtypes, thus providing a basis for a staging classification in BD. METHODS: Latent Class Analysis was applied to multiple predictors of functional outcome in a sample of 106 remitted adults with BD. RESULTS: We identified two subtypes of patients presenting "good" (n=50; 47.6%) and "poor" (n=56; 52.4%) outcome. Episode density, level of residual depressive symptoms, estimated verbal intelligence and inhibitory control emerged as the most significant predictors of subtype membership at the p<0.05 level. Their odds ratio (OR) and confidence interval (CI) with reference to the "good" outcome group were: episode density (OR=4.622, CI 1.592-13.418), level of residual depressive symptoms (OR=1.543, CI 1.210-1.969), estimated verbal intelligence (OR=0.969; CI 0.945-0.995), and inhibitory control (OR=0.771, CI 0.656-0.907). Age, age of onset and duration of illness were comparable between prognostic groups. LIMITATIONS: The longitudinal stability or evolution of the subtypes was not tested. CONCLUSIONS: Our findings provide the first empirically derived staging classification of BD based on two underlying dimensions, one for illness severity and another for cognitive function. This approach can be further developed by expanding the dimensions included and testing the reproducibility and prospective prognostic value of the emerging classes. Developing a disease staging system for BD will allow individualised treatment planning for patients and selection of more homogeneous patient groups for research purposes

Perfil antropométrico, composición corporal y somatotipo de remeros tradicionales de élite: Estudio transversal

Introduction: Given the importance of body composition in sports performance, it is vital to have references of elite athletes which serve as a guide when it comes to overseeing diet and training.The aim of this study was to describe the anthropometric values of an elite team of traditional rowers in order to build an anthropometric profile in this sport. Material and methods: A cross-sectional design with twenty elite, male traditional rowers aged at 29.3 (3.6) years reported to the laboratory on a single day at the start of the competitive season. Height, wingspan, body mass, 8 skinfolds, 2 bone diameters and 6 perimeters were measured by the same internationally certified anthropometrist. Anthropometric measurements were taken following the International Society of Advancement of Kinanthropometry (ISAK) protocol. Fat mass was calculated using different equations for athletes and muscle mass using the Lee equation. For the somatotype components, the Carter and Heath equation was applied.Results: elite traditional rowers had a wingspan of 189 (5.8) cm, body fat percentage of 8.0 (1.2)% (Carter), 8.0 (1.8) (Withers), 7.0 (1.2)% (Yuhasz), and 10.9 (1.1)% (Faulkner). Muscle mass was 43.3 (2.4)% (Lee). The somatotype was endo-mesomorphic with endomorphy values of 3.5 (0.4), mesomorphy 4.7 (0.6) and ectomorphy 2.4 (3.5).Conclusion: These results suggest that wingspan seems to be of great importance for elite traditional rowers; while average height may not be as important for performance as wingspan. Meanwhile, reducing body fat percentage is likely to be beneficial in order to achieve elite rowing status.Introducción: Debido a la importancia que la composición corporal tiene en el rendimiento deportivo es necesario disponer de referencias de deportistas élite que sirvan de guía a la hora de orientar la dieta y el entrenamiento. Material y métodos: El estudio fue diseñado como un estudio transversal que incluyó a veinte remeros tradicionales de élite de 29,3(3,6) años de edad que acudieron al laboratorio un sólo día al comienzo del período competitivo. La altura, envergadura, masa corporal, 8 pliegues cutáneos, 2 diámetros óseos y 6 perímetros fueron determinados por el mismo antropometrista internacionalmente certificado. Las medidas fueron recogidas siguiendo el protocolo de la Sociedad Internacional para el avance de la Cineantropometría (ISAK). La masa grasa se calculó utilizando diferentes ecuaciones para deportistas, y la masa muscular mediante la ecuación de Lee. Los componentes del somatotipo fueron estimados mediante la ecuación Carter y Heath.Resultados: Se observó que los remeros de elite tenían una envergadura de 189 (5,8) cm, un porcentaje de grasa corporal de 8,0 (1,2)% según las ecuaciones de Carter, 8,0 (1,8) de Withers; 7,0 (1,2)% de Yuhasz, y 10,9 (1,1)% de Faulkner. La masa muscular fue de 43,3 (2,4)% según la ecuación de Lee. El somatotipo fue endo-mesomorfo con valores de endomorfia de 3,5(0,4), mesomorfia de 4,7(0,6) y ectomorfia de 2,4 (3,5). Conclusiones: Estos resultados sugieren que la envergadura parece ser de gran importancia para los remeros de élite, mientras que la altura promedio puede no ser tan importante para el rendimiento. Por su parte, reducir el porcentaje de grasa corporal es probablemente beneficioso para lograr un buen rendimiento en este deporte

Distribution of melanopsin positive neurons in pigmented and albino mice: evidence for melanopsin interneurons in the mouse retina.

Here we have studied the population of intrinsically photosensitive retinal ganglion cells (ipRGCs) in adult pigmented and albino mice. Our data show that although pigmented (C57Bl/6) and albino (Swiss) mice have a similar total number of ipRGCs, their distribution is slightly different: while in pigmented mice ipRGCs are more abundant in the temporal retina, in albinos the ipRGCs are more abundant in superior retina. In both strains, ipRGCs are located in the retinal periphery, in the areas of lower Brn3a(+)RGC density. Both strains also contain displaced ipRGCs (d-ipRGCs) in the inner nuclear layer (INL) that account for 14% of total ipRGCs in pigmented mice and 5% in albinos. Tracing from both superior colliculli shows that 98% (pigmented) and 97% (albino) of the total ipRGCs, become retrogradely labeled, while double immunodetection of melanopsin and Brn3a confirms that few ipRGCs express this transcription factor in mice. Rather surprisingly, application of a retrograde tracer to the optic nerve (ON) labels all ipRGCs, except for a sub-population of the d-ipRGCs (14% in pigmented and 28% in albino, respectively) and melanopsin positive cells residing in the ciliary marginal zone (CMZ) of the retina. In the CMZ, between 20% (pigmented) and 24% (albino) of the melanopsin positive cells are unlabeled by the tracer and we suggest that this may be because they fail to send an axon into the ON. As such, this study provides the first evidence for a population of melanopsin interneurons in the mammalian retina

The efficacy of psychotherapy, pharmacotherapy and their combination on functioning and quality of life in depression:a meta-analysis

BACKGROUND: There is growing recognition of the importance of both functioning and quality of life (QoL) outcomes in the treatment of depressive disorders, but the meta-analytic evidence is scarce. The objective of this meta-analysis of randomized controlled trials (RCTs) was to determine the absolute and relative effects of psychotherapy, pharmacotherapy and their combination on functioning and QoL in patients with depression. METHOD: One hundred and fifty-three outcome trials involving 29 879 participants with depressive disorders were identified through database searches in Pubmed, PsycINFO and the Cochrane Central Register of Controlled Trials. RESULTS: Compared to control conditions, psychotherapy and pharmacotherapy yielded small to moderate effect sizes for functioning and QoL, ranging from g = 0.31 to g = 0.43. When compared directly, initial analysis yielded no evidence that one of them was superior. After adjusting for publication bias, psychotherapy was more efficacious than pharmacotherapy (g = 0.21) for QoL. The combination of psychotherapy and medication performed significantly better for both outcomes compared to each treatment alone yielding small effect sizes (g = 0.32 to g = 0.39). Both interventions improved depression symptom severity more than functioning and QoL. CONCLUSION: Despite the small number of comparative trials for some of the analyses, this study reveals that combined treatment is superior, but psychotherapy and pharmacotherapy alone are also efficacious for improving functioning and QoL. The overall relatively modest effects suggest that future tailoring of therapies could be warranted to better meet the needs of individuals with functioning and QoL problems