Oxidative stress parameters in serum and low density lipoproteins of Hashimoto's thyroiditis patients with subclinical and overt hypothyroidism

Background: Although prooxidant and antioxidant status were reported to be changed in clinical and experimental hypothyroidism, obtained results are conflicting. In addition, in subclinical hypothyroidism, scarced and controversial data are available about oxidative stress. Therefore, we aimed to investigate prooxidant-antioxidant status only in Hashimoto's thyroiditis (HT) patients with subclinical (sHT) and overt hypothyroidism (oHT)

Voice characteristics associated with polycystic ovary syndrome

Objectives/HypothesisTo test the assumption that voice is changed in polycystic ovary syndrome (PCOS) and identify changes that occur

High circulating levels of sICAM-1 and sVCAM-1 in the patients with Hashimoto's thyroiditis

Objective: To investigate whether soluble intercellular adhesion molecule-1 (sICAM-1) and soluble vascular cell adhesion molecule-1 (sVCAM-1) levels are increased in euthyroid patients with Hashimoto's thyroiditis (HT) and whether they are associated with thyroid autoimmunity and metabolic parameters. Design: Cross-sectional. Subjects and Methods: In total, 80 euthyroid patients with HT and 80 age- and body mass index (BMI)-matched control participants were included. Serum sICAM-1, sVCAM-1, free triiodothyronine (fT3), free thyroxine (fT4), thyroid-stimulating hormone (TSH), thyroid peroxidase antibody (anti-TPO), thyroglobulin antibody (anti-TG), fasting blood glucose (FBG), insulin, and lipid levels and homeostasis model assessment for insulin resistance (HOMA-IR) were assessed in all participants. Results: The patients with HT had significantly higher levels of sICAM-1 and sVCAM-1 than controls (both p < 0.001). The difference was sustained after adjustment for TSH and levothyroxine use. Regression analysis demonstrated that sICAM-1 was related to anti-TPO (p < 0.001), and sVCAM-1 was related to both anti-TPO and-TG (p < 0.001 and p = 0.03, respectively); this relationship was sustained after adjustment for age and BMI. Although FBG and HOMA-IR were higher in the HT group, logistic regression analysis revealed that there was no effect of anti-TPO, anti-TG, sICAM-1, sVCAM-1, and C-reactive protein (CRP) on the occurrence of high FBG and high HOMA-IR. Conclusion: sICAM-1 and sVCAM-1 levels were significantly elevated in the patients with euthyroid HT and correlated closely with thyroid autoimmunity. However, soluble adhesion molecules had no relation with glucose metabolism parameters in the HT patients

Arg25Pro (c.915G > C) polymorphism of transforming growth factor beta 1 gene increases the risk of developing Graves' disease

Background: Graves' disease (GD) arises due to complex interactions between genetic and environmental factors. Transforming growth factor beta 1 (TGF beta 1) is required to maintain immune homeostasis, and is implicated in lymphocyte infiltration, thyroid follicular cell hyperplasia, and production of autoantibody in the thyroid gland of patients with GD

Vascular endothelial growth factor polymorphisms increase the risk of developing Graves' disease

Background: Graves' disease (GD) is a consequence of genetic and environmental factors. Vascular endothelial growth factor (VEGF) is a strong angiogenic and mitogenic factor, which plays a key role in lymphocyte infiltration, and hypervascularization in the thyroid gland of patients with GD

ICAM1 K469E and E-selectin S128R polymorphisms could predispose to increased autoantibody production and TSH suppression in Graves' disease

The etiopathogenesis of Graves' disease (GD) has not been clearly elucidated although the role of chronical inflammation and endothelial dysfunction has been established. Adhesion molecules such as intercellular adhesion molecule 1 (ICAM1), vascular cell adhesion molecule 1 (VCAM1), and E-selectin are secreted from vascular endothelium and promote accummulation of leukocytes in damaged endothelial areas. This study examined the possible association of ICAM1 (G241R and K469E), VCAM1 (T-1591C and T-833C), and E-selectin (S128R) single nucleotide polymorphisms (SNPs) with the occurence of GD. ICAM1 (G241R and K469E), VCAM1 (T-1591C and T-833C), and E-selectin (S128R) SNPs in DNA from peripheral blood leukocytes of 171 patients with GD and 259 healthy controls were investigated by real-time PCR combined with melting curve analysis using fluorescence-labeled hybridization probes. We did not find significant differences in the distributions of studied polymorphisms, nor in the haplotype frequencies between patients with GD and healthy control. However, the anti-TPO levels in E-selectin 128R allele carrying subjects (SR + RR) were higher than S128S genotype (p < 0.05). In addition, the decline of TSH levels was more prominent in ICAM1 469 E carrying subjects (KE + EE) in comparison with wild homozygotes (p < 0.05). Although there is not assosiation between ICAM1 (G241R and K469E), VCAM1 (T-1591C and T-833C), and E-selectin (S128R) SNPs and susceptibility to GD, higher anti-TPO in E-selectin 128 SR + RR, and lower TSH in ICAM1 469 KE + EE subjects suspect that these genotypes are prone to increased antithyroid autoantibody production with more accentuated TSH suppression in GD. Further studies with a larger cohort, analyzing other polymorphisms in ICAM, VCAM1 and E-selectin genes are necessary to support our observations

Arg25Pro (c.915G > C) polymorphism of transforming growth factor beta 1 gene suggests an association with increased risk for Hashimoto's thyroiditis

Background: The etiopathogenesis of Hashimoto's thyroiditis (HT) - has not been clearly elucidated although the role of chronic inflammation, endothelial dysfunction, and imbalance between pro- and anti-inflammatory cytokines has been established. Transforming growth factor beta 1 (TGF beta 1) is required to maintain immune homeostasis, and is implicated in lymphocyte infiltration, production of autoantibodies and thyrocyte destruction seen in patients with HT

Evaluation of the Relationship between Insulin Resistance and Selenoprotein P in Patients with Polycystic Ovary Syndrome

Polikistik Over Sendromlu Hastalarda İnsülin Direnci ve Selenoprotein P İlişkisinin DeğerlendirilmesiAyşenur Özderya1, İbrahim Yılmaz2, Şevin Demir3, Şule Temizkan1, Mehmet Sargın4, Mehmet Ali Ustaoğlu2, Kadriye Aydın11Sağlık Bilimleri Üniversitesi, Kartal Dr. Lütfi Kırdar Eğitim Ve Araştırma Hastanesi, Endokrinoloji Ve Metabolizma Hastalıkları Bölümü,i̇stanbul, Türkiye2Sağlık Bilimleri Üniversitesi, Kartal Dr. Lütfi Kırdar Eğitim Ve Araştırma Hastanesi, İç Hastalıkları Kliniği, İstanbul, Türkiye3Sağlık Bilimleri Üniversitesi, Kartal Dr Lütfi Kırdar Eğitim ve Araştırma Hastanesi, Aile Hekimliği Kliniği, İstanbul, Türkiye4Medeniyet Üniversitesi Tıp Fakültesi, Aile Hekimliği Kliniği, İstanbul, TürkiyeGİRİŞ ve AMAÇ: Polikistik over sendromu (PKOS) doğurganlık çağındaki kadınlarda en sık görülen ve insülin direnci (IR) ile karakterize bir bozukluktur. Selenoprotein P(SeP) de, insülin direnciyle ilişkili bir hepatokindir. Bu çalışmada PKOS’da SeP düzeylerini belirlemeyi ve IR ile ilişkisini araştırmayı amaçladık.&nbsp;YÖNTEM ve GEREÇLER: Çalışmada 27 hastayla yaş ve vücut kitle indeksi (VKI) eşleştirilmiş 27 sağlıklı kontrolün demografik özellikleri, antropometrik ölçümleri ve biyokimyasal parametreleri değerlendirildi. IR ve serbest androjen indeksi (FAI) hesaplandı. SeP ile biyokimyasal ve antropometrik parametrelerin korelasyonu yapıldı.BULGULAR: Hasta ve kontroller arasında açlık insülini ve HOMA-IR anlamlı farklıyken (her iki p&lt;0.05), SeP düzeyleri benzerdi (sırasıyla, 1.05±0.7ng/mL ve 1.61±1.9ng/mL, p=0.7). Her iki grupta da SeP ile HOMA-IR arasında korelasyon saptanmadı. PCOS grubunda SeP ile bel çevresi arasında negatif korelasyon mevcutken (p=0.03, r=-0.485), kontrol grubunda izlenmedi. Kontrol grubunda ise SeP ile VKI ve yağ yüzdesi arasında negatif korelasyon mevcutken (sırasıyla r=-0.506, p=0.007 ve r=-0.643, p=0.024), PCOS grubunda izlenmedi. Ayrıca hastalarda testosteron ile SeP arasında anlamlı pozitif korelasyon saptandı (r=0,456, p=0,017).&nbsp;TARTIŞMA ve SONUÇ: Hasta ve kontroller arasında SeP düzeyleri benzer bulundu ve PKOS’da SeP ile IR arasında bir ilişki saptanmadı. Ancak PKOS'da SeP’nin bel çevresi ve testosteron ile korelasyonu olası bir metabolik ilişkiyi akla getirmektedir.&nbsp;Anahtar Kelimeler:&nbsp;İnsülin direnci, polikistik over sendromu, selenoprotein P, vücut kitle indeksi, vücut yağ yüzdesi.</p

Measurment of Ankle-Brachial Pressure Index, Homocystein Level, and Evaluation of Coronary Artery Disease Association with Other Macrovascular Diseases in Patients with Myocardial Infarction

We aimed to measure ankle-brachial pressure index (ABPI) and homocysteine levels, and to evaluate frequency of cerebrovascular disease (CVD) and/or peripheral arterial disease (PAD) combination of patients with myocardial infarction (MI). 39 patients (26 males, 13 females) with acute or subacute MI and 36 control cases (9 male, 27 female) were included in the study. ABPI and homocysteine levels were measured, and bilateral carotid-vertebral and bilateral lower extremity arterial Doppler ultrasonography, and coronary angiography were examined. Homocysteine was significantly higher in patient group than control group (p=0.0001). The ABPI was not significantly different in two groups (p=0.428). However the frequency of patients with lower ABPI (&#8804;0.9) was significantly higher compared to the frequency of control patients with lower ABPI (25.6% and 3%, respectively; p=0.02). The combination of the atherosclerotic findings in the carotid artery and coronary artery disease (CAD) were found significantly higher compared to that of the bilateral lower extremity (59%, 25.6%). Screening of CVD should be done in patients with MI history. Determining carotid arterial lesions may be useful for the early diagnosis and treatment of any possible CVD in cases with CAD. Studies with larger numbers of cases are needed. [Med-Science 2013; 2(4.000): 896-906

The combination of nterleukin-10-1082 and tumor necrosis factor alpha-308 or interleukin-6-174 genes polymorphisms suggests an association with susceptibility to Hashimoto's thyroiditis

Background: The etiopathogenesis of Hashimoto's thyroiditis (HT) has not been clearly elucidated although the role of chronical inflammation and endothelial dysfunction has been established. The imbalance between pro- and anti-inflammatory cytokines may play a role in the etiology. The aim of the present study was to investigate whether cytokine gene polymorphisms are associated with HT, and to evaluate the relationship between genotypes and clinical/laboratory manifestation of HT