Pan-cancer Alterations of the MYC Oncogene and Its Proximal Network across the Cancer Genome Atlas

Although the MYC oncogene has been implicated in cancer, a systematic assessment of alterations of MYC, related transcription factors, and co-regulatory proteins, forming the proximal MYC network (PMN), across human cancers is lacking. Using computational approaches, we define genomic and proteomic features associated with MYC and the PMN across the 33 cancers of The Cancer Genome Atlas. Pan-cancer, 28% of all samples had at least one of the MYC paralogs amplified. In contrast, the MYC antagonists MGA and MNT were the most frequently mutated or deleted members, proposing a role as tumor suppressors. MYC alterations were mutually exclusive with PIK3CA, PTEN, APC, or BRAF alterations, suggesting that MYC is a distinct oncogenic driver. Expression analysis revealed MYC-associated pathways in tumor subtypes, such as immune response and growth factor signaling; chromatin, translation, and DNA replication/repair were conserved pan-cancer. This analysis reveals insights into MYC biology and is a reference for biomarkers and therapeutics for cancers with alterations of MYC or the PMN. We present a computational study determining the frequency and extent of alterations of the MYC network across the 33 human cancers of TCGA. These data, together with MYC, positively correlated pathways as well as mutually exclusive cancer genes, will be a resource for understanding MYC-driven cancers and designing of therapeutics

Whatever happened to the third paradigm? Exploring mixed methods research designs in sport and exercise psychology

In the past, quantitative and qualitative approaches to research were portrayed as being incompatible, if not mutually exclusive. More recently, however, researchers have explored the possible complementarity of these approaches through mixed methods research (MMR) the so-called third research paradigm. The purpose of this paper is to explore the nature and implications of mixed methods designs for research in sport and exercise psychology. Having sketched the nature and origins of MMR, we highlight some advantages it offers to researchers in sport and exercise psychology. After that, we conclude by identifying some barriers to progress in using mixed methods research in this latter field.Author has checked copyrightkpw13/5/1