Elderly and aged asthma have different characteristics: results of a multicenter study

Background/aim: Characteristics of asthma in the elderly population is not well-known. The aim of the present study was to evaluate asthma in the elderly population, to compare disease characteristics between patients diagnosed <60 (aged asthma) and ≥60 (elderly asthma) years of age. Materials and methods: The study was a prospective, multicenter, cross-sectional type. A questionnaire was filled out to patients 60 years of age and over, that have been followed for asthma for at least 3 months. Asthma Control Test (ACT), eight-item Morisky Medication Adherence Scale (MMAS-8) was filled out, inhaler device technique was assessed. Results: A total of 399 patients were included from 17 tertiary care centers across the country. Mean age was 67.11 years and 331 (83%) were female. The age at asthma diagnosis was ≥60 in 146 (36.6%) patients. Patients diagnosed ≥60 years were older (p < 0.001), had higher education level (p < 0.001), more commonly had first-degree relative with asthma (p = 0.038), asthma related comorbidities (p = 0.009) and accompanying rhinitis/rhinosinusitis (p = 0.005), had better asthma control (p = 0.001), were using less controller medications (p = 0.014). Inhaler technique was correct in 37% of the patients with no difference in between the groups. Treatment compliance was better in elderly asthma patients (p < 0.001). In the multivariate logistic regression analysis, having well-controlled asthma (odds ratio = 1.61, CI = 1.04-2.51), and high medication adherence rate (odds ratio = 2.43, CI = 1.48-4.0) were associated with being in the elderly asthma group. Conclusion: The characteristics of asthma are different among patients aged 60 years and over which seems to be related to onset age of asthma. In our cohort, the elderly asthma patients had higher education level, and treatment adherence and asthma control was better. Patients diagnosed ≥60 years of age did not have more severe disease

Çoklu ilaç dirençli salmonella infantislerin plazmidlerinin karakterizasyonu.

The usage and misusage of antibiotics in poultry, food-producing animals and human diseases have led to transmission of conjugative plasmids carrying antibiotic resistance genes from one microorganism to another, especially to the pathogenic bacteria. Multi-drug resistant Salmonella enterica serovar Infantis, an emerging serotype in poultry, has been spreading all around the world in a decade. Moreover, commensal microorganisms such as commensal Escherichia coli in the gut microbiota, functioning as a reservoir of antimicrobial resistance, of the warm-blooded hosts acquire antibiotic resistance by plasmid from Salmonella enterica serovars. The aim of this study was to identify the conjugative transfer of plasmids including multi-drug resistance genes from Salmonella enterica serovar Infantis to commensal susceptible E. coli in both of phenotypic and genotypic level. Salmonella Infantis isolates with different antibiotic resistance profiles involving streptomycin, sulfisoxazole, ampicillin, trimethoprim/sulfamethoxazole, kanamycin, chloramphenicol, ciprofloxacin, cephalotin, nalidixic acid and tetracycline were selected as donor bacteria (n=10), while susceptible non-pathogenic E. coli isolate was used as a recipient. Applying filter paper mating procedure was used for conjugation. Transconjugant E. coli colonies were screened by phenotypic and genotypic methods. First, yellow colonies representing transconjugant E. coli colonies were determined on the selective XLD agar containing 10 μg/ml tetracycline in phenotypic level. After that, conjugation genes (i.e., traA, traE, traL, traJ, traG, traH, traI, traM, traR, traY, rfaG, rfaI, rfaJ, rfaL and rfbP), and the incompatibility group of plasmids were screened by polymerase chain reaction (PCR) in genotypic level. This study contributes to recent and further studies related to the conjugative transmission of antibiotic resistance genes in Salmonella and E. coli, which is an arising serious problem worldwide.M.S. - Master of Scienc

SIĞIRLARIN LENF DÜĞÜMLERİNDEN İZOLE EDİLEN SALMONELLA İZOLATLARININ FENOTİPİK VE GENETİK OLARAK KARAKTERİZASYONU

SIĞIRLARIN LENF DÜĞÜMLERİNDEN İZOLE EDİLEN SALMONELLA İZOLATLARININ FENOTİPİK VE GENETİK OLARAK KARAKTERİZASYON

FEN BİLİMLERİ ENSTİTÜSÜ/LİSANSÜSTÜ TEZ PROJESİ

SIĞIRLARIN LENF DÜĞÜMLERİNDE İZOLE EDİLEN SALMONELLA SUŞLARININ FENOTİPİK VE GENOTİPİK KARAKTERİZASYON