Realization of Strategic Analysis of Manutan s.r.o. Company

Import 22/07/2015V této práci je popsána strategická analýza firmy Manutan s. r. o. Teoretická část obsahuje vymezení základních pojmů a analýz. Praktická část je zaměřená na aplikaci analýz, vymezení závěru a doporučení pro analyzovanou firmu.In this work is described strategic analysis of the company Manutan s.r.o. The theoretical part defines the basic concepts and analysis. The practical part is devoted to the application of analysis, definition of conclusions and recommendations for the analyzed company.115 - Katedra managementuvýborn

Lzts1 controls both neuronal delamination and outer radial glial-like cell generation during mammalian cerebral development

発生期の大脳で分化細胞と未分化細胞の移動開始をもたらす共通の分子を解明 --「大脳のシワ」形成に貢献する外側放射状グリアはどのように誕生するか--. 京都大学プレスリリース. 2019-07-08.In the developing central nervous system, cell departure from the apical surface is the initial and fundamental step to form the 3D, organized architecture. Both delamination of differentiating cells and repositioning of progenitors to generate outer radial glial cells (oRGs) contribute to mammalian neocortical expansion; however, a comprehensive understanding of their mechanisms is lacking. Here, we demonstrate that Lzts1, a molecule associated with microtubule components, promotes both cell departure events. In neuronally committed cells, Lzts1 functions in apical delamination by altering apical junctional organization. In apical RGs (aRGs), Lzts1 expression is variable, depending on Hes1 expression levels. According to its differential levels, Lzts1 induces diverse RG behaviors: planar division, oblique divisions of aRGs that generate oRGs, and their mitotic somal translocation. Loss-of-function of lzts1 impairs all these cell departure processes. Thus, Lzts1 functions as a master modulator of cellular dynamics, contributing to increasing complexity of the cerebral architecture during evolution

Microbicidal effects of weakly acidified chlorous acid water against feline calicivirus and Clostridium difficile spores under protein-rich conditions.

Sanitation of environmental surfaces with chlorine based-disinfectants is a principal measure to control outbreaks of norovirus or Clostridium difficile. The microbicidal activity of chlorine-based disinfectants depends on the free available chlorine (FAC), but their oxidative potential is rapidly eliminated by organic matter. In this study, the microbicidal activities of weakly acidified chlorous acid water (WACAW) and sodium hypochlorite solution (NaClO) against feline calcivirus (FCV) and C. difficile spores were compared in protein-rich conditions. WACAW inactivated FCV and C. difficile spores better than NaClO under all experimental conditions used in this study. WACAW above 100 ppm FAC decreased FCV >4 log10 within 30 sec in the presence of 0.5% each of bovine serum albumin (BSA), polypeptone or meat extract. Even in the presence of 5% BSA, WACAW at 600 ppm FAC reduced FCV >4 log10 within 30 sec. Polypeptone inhibited the virucidal activity of WACAW against FCV more so than BSA or meat extract. WACAW at 200 ppm FAC decreased C. difficile spores >3 log10 within 1 min in the presence of 0.5% polypeptone. The microbicidal activity of NaClO was extensively diminished in the presence of organic matter. WACAW recovered its FAC to the initial level after partial neutralization by sodium thiosulfate, while no restoration of the FAC was observed in NaClO. These results indicate that WACAW is relatively stable under organic matter-rich conditions and therefore may be useful for treating environmental surfaces contaminated by human excretions

Effect of BSA on FCV inactivation by WACAW and NaClO.

<p>The virucidal activity against FCV F9 was examined without BSA (A) or with 0.5% (w/v) BSA (B) or 5.0% (w/v) BSA (C). The remaining FCV infectivity after treatment with the test sanitizers for the indicated times was quantified by plaque assay. Data are expressed as means ± standard deviations obtained from three independent experiments. * Significantly different from the data in NaClO with 25 ppm (A), 200 ppm (B) and 3,000 ppm (C) at each time point (<i>p</i> <0.01).</p

Effect of meat extract on FCV inactivation by WACAW and NaClO.

<p>The virucidal activity against FCV F9 was examined in reaction mixtures containing 0.5% meat extract (w/v). The remaining FCV infectivity after treatment with the test sanitizers for the indicated times was quantified by plaque assay. Data are expressed as means ± standard deviations obtained from three independent experiments. Significant differences from the 1,000 ppm NaClO at each time point were expressed by * (<i>p</i> <0.01).</p

<i>C</i>. <i>difficile s</i>pore inactivation by WACAW and NaClO.

<p>The sporicidal activity against <i>C</i>. <i>diffcile</i> 630 spores was examined in reaction mixtures without (A) or with 0.5% (w/v) polypeptone (B). The remaining viable <i>C</i>. <i>difficile</i> spores after treatment with the test sanitizers for the indicated times were quantified using a standard plate assay with BHIS supplemented with taurocholic acid. Data are expressed as means ± standard deviations obtained from three independent experiments. Significant differences from the 200 ppm NaClO at each time point were expressed by * (<i>p</i> <0.01) or ** (<i>p</i> <0.05).</p

Changes of TC, FAC and combined chlorine levels in the test sanitizers after contact with BSA.

<p>Changes of TC, FAC and combined chlorine levels in the test sanitizers after contact with BSA.</p

女子大学生による子宮頸がん啓発活動プロジェクトの３年間の活動報告

報告Reports　本学看護学部では、2019 年度に学生と教員が協働して聖隷婦人科啓発活動プロジェクトを発足した。2020 年度には本プロジェクトは、「地域実践アクティブラーニングⅠ・Ⅱ・Ⅲ」の科目に位置付けられ、活動内容を子宮頸がん啓発活動とし、科目の到達目標達成に向けて活動を行った。具体的には、子宮頸がん啓発ツールの作成、自治体が行っているがん予防啓発活動への参画、高校生を対象とした子宮頸がんや検診に関する出張授業などである。学生は、能動的に学修に取り組み、教員や学外の多様な人たちと連携・協働して活動を展開し、科目の到達目標を概ね達成することができた。今後は、本プロジェクトを継続させるための方略や更なる活動の展開方法に関して検討していくことが課題である