61 research outputs found
Machine Learning-based Linear regression way to deal with making data science model for checking the sufficiency of night curfew in Maharashtra, India
The birthplace of the novel Covid-19 sickness or COVID-19 began its spread around Wuhan city, China. The spread of this novel infection sickness began toward the start of December 2019. The Covid-19 illness spreads from one individual to another through hacking, sniffling, etc. To stop the spreading of the novel Covid-19 infection the distinctive nation has presented diverse strategies. Some regularly utilized methods are lockdown, night curfew, etc. The fundamental intention of the systems was to stop the social events and leaving homes without serious issues. Utilizing a diverse system Covid-19 first stage can address for saving individuals. Presently the second influx of this novel Covid illness has begun its top from the mid of April-May. The second convergence of this novel Covid disorder flooded all through the world and in India too. To stop the spread of this novel Covid sickness India's richest state Maharashtra government constrained the decision of night curfew. In this paper, we are taking as a relevant examination the night curfew on a schedule of Maharashtra. Here, we study that this system may or may not be able to stop the spread of pandemics.
We are using the Machine learning(ML) approach to managing regulate study this case. ML has various systems yet among all of those here we use Linear Regression for the current circumstance. The reproduced insight that readies the plan orchestrated to learn with no other person. Linear Regression is the affirmed strategy for looking over the connection between two sections. Between the two segments, one is astute and another is a seen variable
Variations in the SDN Loop of Class A Beta-Lactamases: A Study of the Molecular Mechanism of BlaC (Mycobacterium tuberculosis) to Alter the Stability and Catalytic Activity Towards Antibiotic Resistance of MBIs
The emergence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis calls for an immediate search for novel treatment strategies. Recently, BlaC, the principal beta-lactamase of Mycobacterium tuberculosis, was recognized as a potential therapeutic target. BlaC belongs to Ambler class A, which is generally susceptible to the beta-lactamase inhibitors currently used in clinics: tazobactam, sulbactam, and clavulanate. Alterations at Ser130 in conserved SDN loop confer resistance to mechanism-based inhibitors (MBIs) commonly observed in various clinical isolates. The absence of clinical evidence of S130G conversion in M. tuberculosis draws our attention to build laboratory mutants of S130G and S130A of BlaC. The study involving steady state, inhibition kinetics, and fluorescence microscopy shows the emergence of resistance against MBIs to the mutants expressing S130G and S130A. To understand the molecular reasoning behind the unavailability of such mutation in real life, we have used circular dichroism (CD) spectroscopy, differential scanning calorimetry (DSC), molecular dynamics (MD) simulation, and stability-based enzyme activity to compare the stability and dynamic behaviors of native and S130G/A mutant form of BlaC. A significant decrease in melting temperature (BlaC T M 60°C, S130A T M 50°C, and S130G T M 45°C), kinetic instability at higher temperature, and comparative dynamic instability correlate the fact that resistance to beta-lactam/beta-lactamase inhibitor combinations will likely not arise from the structural alteration of BlaC, therefore establishing confidence that this therapeutic modality can be potentially applied as a part of a successful treatment regimen against M. tuberculosis
Evidence of a compensated semimetal with electronic correlations at the CNP of twisted double bilayer graphene
Recently, magic-angle twisted bilayer graphene (MATBLG) has shown the
emergence of various interaction-driven novel quantum phases at the
commensurate fillings of the moir'e superlattice, while the charge neutrality
point (CNP) remains mostly a vanilla insulator. Here, we show an emerging phase
of nearly compensated semimetallicity at the CNP of twisted double bilayer
graphene (TDBLG), a close cousin of MATBLG, with signatures of electronic
correlation. Using electrical and thermal transport, we find almost two orders
of magnitude enhancement of the thermopower in magnetic fields much smaller
than the extreme quantum limit, accompanied by a large magnetoresistance() at CNP. This provides indisputable experimental evidence that TDBLG
near CNP is a compensated semimetal. Moreover, at low temperatures, we observe
an unusual sublinear temperature dependence of resistance. A recent theory
predicts the formation of an excitonic metal near CNP, where small electron and
hole pockets coexist. We understand the sublinear temperature dependence in
terms of critical fluctuations in this theory
Probing the dynamics of an optically trapped particle by phase sensitive back focal plane interferometry
The dynamics of an optically trapped particle are often determined by
measuring intensity shifts of the back-scattered light from the particle using
position sensitive detectors. We present a technique which measures the phase
of the back-scattered light using balanced detection in an external Mach-Zender
interferometer scheme where we separate out and beat the scattered light from
the bead and that from the top surface of our trapping chamber. The technique
has improved axial motion resolution over intensity-based detection, and can
also be used to measure lateral motion of the trapped particle. In addition, we
are able to track the Brownian motion of trapped 1 and 3 m diameter beads
from the phase jitter and show that, similar to intensity-based measurements,
phase measurements can also be used to simultaneously determine displacements
of the trapped bead as well as the spring constant of the trap. For lateral
displacements, we have matched our experimental results with a simulation of
the overall phase contour of the back-scattered light for lateral displacements
by using plane wave decomposition in conjunction with Mie scattering theory.
The position resolution is limited by path drifts of the interferometer which
we have presently reduced to obtain a displacement resolution of around 2 nm
for 1.1 m diameter probes by locking the interferometer to a frequency
stabilized diode laser.Comment: 10 pages, 7 figure
Computational Assessment of the Biocompatibility of Two-Dimensional g‑C<sub>3</sub>N<sub>3</sub> Toward Lipid Membranes
One of the most recent additions to the family of two-dimensional
(2D) materials, graphitic C3N3 (g-C3N3), has been considered a viable contender for biomedical
applications, although its potential toxicity remains elusive. We
perform all-atom molecular dynamics simulations to decipher the interactions
between model lipid membranes and g-C3N3 as
a first step toward exploring the cytotoxicity induced at the nanoscale.
We show that g-C3N3 can easily insert into the
cellular membranes following a multistage mechanism consisting of
simultaneous desolvation of the 2D material along with enrichment
of nanomaterial–lipid interactions. Free energy calculations
indicate that g-C3N3 is more stable in a membrane-bound
state compared to an aqueous solution; however, the insertion of the
material does not disturb the structural integrity of lipid membranes.
After being inserted into a membrane, g-C3N3 is unlikely to be released into the cellular environment and is
incapable of extracting lipid molecules from the membrane. The nature
of interaction between the 2D material and membranes is found to be
independent of the nanomaterial size. Also, the performance of g-C3N3 toward biomolecular delivery is shown to be
significantly improved compared to the state-of-the-art 2D materials
graphene and hexagonal boron nitride (h-BN). It is
revealed that, the affinity of g-C3N3 toward
lipid membranes is weaker compared to the nanotoxic graphene and h-BN, while being marginally higher than h2D–C2N, which in turn, increases the biocompatibility
of the material, thereby brightening its future as a noncytotoxic
material for forthcoming biomedical applications
New symmetrical dinucleating ligand based assembly of bridged dicopper(II) and dizinc(II) centers: Synthesis, structure, spectroscopy, magnetic properties and glycoside hydrolysis
Two dinuclear copper(II) complexes Li(H2O)(3)(CH3OH)](4)Cu2Br4]Cu-2(cpdp)(mu-O2CCH3)](4)(OH)(2) (1), Cu (H2O)(4)]Cu-2(cpdp)(mu-O2CC6H5)](2)Cl-2 center dot 5H(2)O (2), and a dinuclear zinc(II) complex Zn-2(cpdp)(mu-O2CCH3)] (3) have been synthesized using pyridine and benzoate functionality based new symmetrical dinucleating ligand, N, N'-Bis2-carboxybenzomethyl]-N, N'-Bis2-pyridylmethyl]-1,3-diaminopropan-2-ol (H(3)cpdp). Complexes 1, 2 and 3 have been synthesized by carrying out reaction of the ligand H3cpdp with stoichiometric amounts of Cu-2(O2CCH3)(4)(H2O)(2)], CuCl2 center dot 2H(2)O/C6H5COONa, and Zn(CH3COO)(2)center dot 2H(2)O, respectively, in methanol in the presence of NaOH at ambient temperature. Characterizations of the complexes have been done using various analytical techniques including single crystal X-ray structure determination. The X-ray crystal structure analyses reveal that the copper(II) ions in complexes 1 and 2 are in a distorted square pyramidal geometry with Cu-Cu separation of 3.455(8) angstrom and 3.492(1)angstrom, respectively. The DFT optimized structure of complex 3 indicates that two zinc(II) ions are in a distorted square pyramidal geometry with Zn-Zn separation of 3.492(8)angstrom. UV-Vis and mass spectrometric analyses of the complexes confirm their dimeric nature in solution. Furthermore, H-1 and C-13 NMR spectroscopic investigations authenticate the integrity of complex 3 in solution. Variable-temperature (2-300 K) magnetic susceptibility measurements show the presence of antiferromagnetic interactions between the copper centers, with J = -26.0 cm(-1) and -23.9 cm(-1) ((H) over cap = -2JS(1)S(2)) in complexes 1 and 2, respectively. In addition, glycosidase-like activity of the complexes has been investigated in aqueous solution at pH similar to 10.5 by UV-Vis spectrophotometric technique using p-nitrophenyl-alpha-D-glucopyranoside (4) and p-nitrophenyl-beta-D-glucopyranoside (5) as model substrates. (C) 2015 Elsevier B.V. All rights reserved
Leishmania donovani Inhibitor of Serine Peptidases 2 Mediated Inhibition of Lectin Pathway and Upregulation of C5aR Signaling Promote Parasite Survival inside Host
Leishmania donovani, the causative agent of Indian visceral leishmaniasis has to face several barriers of the immune system inside the mammalian host for its survival. The complement system is one of the first barriers and consists of a well-balanced network of proteases including S1A family serine proteases (SPs). Inhibitor of serine peptidases (ISPs) is considered as inhibitor of S1A family serine peptidases and is reported to be present in trypanosomes, including Leishmania. In our previous study, we have deciphered the role of ISPs [LdISP1 and L. donovani inhibitor of serine peptidases 2 (LdISP2)] in the survival of L. donovani inside the sandfly midgut. However, the role of theses ISPs in the survival of L. donovani inside mammalian host still remains elusive. In the present study, we have deciphered the inhibitory effect of LdISPs on the host complement S1A serine peptidases, such as C1r/C1s and MASP1/MASP2. Our study suggested that although both rLdISP1 and rLdISP2 inferred strong interaction with C1complex and MBL-associated serine proteases (MASPs) but rLdISP2 showed the stronger inhibitory effect on MASP2 than rLdISP1. Moreover, we found that rLdISP2 significantly reduces the formation of C3, C5 convertase, and membrane attacking complex (MAC) by lectin pathway (LP) resulting in significant reduction in serum mediated lysis of the parasites. The role of LdISP2 on neutrophil elastase-mediated C5aR signaling was also evaluated. Notably, our results showed that infection of macrophages with ISP2-overexpressed Leishmania parasites significantly induces the expression of C5aR both at the transcript and translational level. Simultaneously, infection with ISP2KD parasites results in downregulation of host PI3K/AKT phosphorylation and increased in IL-12 production. Taken together, our findings clearly suggest that LdISP2 promotes parasite survival inside host by inhibiting MAC formation and complement-mediated lysis via LP and by upregulation of C5aR signaling
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