Correlations between plasma variables and the deposition process of Si films from chlorosilanes in low pressure RF plasma of argon and hydrogen

The dissociation of chlorosilanes to silicon and its deposition on a solid substrate in a RF plasma of mixtures of argon and hydrogen were investigated as a function of the macrovariables of the plasma. The dissociation mechanism of chlorosilanes and HCl as well as the formation of Si in the plasma state were studied by sampling the plasma with a quadrupole mass spectrometer. Macrovariables such as pressure, net RF power input and locations in the plasma reactor strongly influence the kinetics of dissociation. The deposition process of microcrystalline silicon films and its chlorine contamination were correlated to the dissociation mechanism of chlorosilanes and HCl

Similarity classes of 3x3 matrices over a local principal ideal ring

In this paper similarity classes of three by three matrices over a local principal ideal commutative ring are analyzed. When the residue field is finite, a generating function for the number of similarity classes for all finite quotients of the ring is computed explicitly.Comment: 14 pages, final version, to appear in Communications in Algebr

Development and Validation of Clinical Whole-Exome and Whole-Genome Sequencing for Detection of Germline Variants in Inherited Disease

Context.-With the decrease in the cost of sequencing, the clinical testing paradigm has shifted from single gene to gene panel and now whole-exome and whole-genome sequencing. Clinical laboratories are rapidly implementing next-generation sequencing-based whole-exome and whole-genome sequencing. Because a large number of targets are covered by whole-exome and whole-genome sequencing, it is critical that a laboratory perform appropriate validation studies, develop a quality assurance and quality control program, and participate in proficiency testing. Objective.-To provide recommendations for wholeexome and whole-genome sequencing assay design, validation, and implementation for the detection of germline variants associated in inherited disorders. Data Sources.-An example of trio sequencing, filtration and annotation of variants, and phenotypic consideration to arrive at clinical diagnosis is discussed. Conclusions.-It is critical that clinical laboratories planning to implement whole-exome and whole-genome sequencing design and validate the assay to specifications and ensure adequate performance prior to implementation. Test design specifications, including variant filtering and annotation, phenotypic consideration, guidance on consenting options, and reporting of incidental findings, are provided. These are important steps a laboratory must take to validate and implement whole-exome and whole-genome sequencing in a clinical setting for germline variants in inherited disorders

Development and Validation of Targeted Next-Generation Sequencing Panels for Detection of Germline Variants in Inherited Diseases.

Context.-The number of targeted next-generation sequencing (NGS) panels for genetic diseases offered by clinical laboratories is rapidly increasing. Before an NGS-based test is implemented in a clinical laboratory, appropriate validation studies are needed to determine the performance characteristics of the test. Objective.-To provide examples of assay design and validation of targeted NGS gene panels for the detection of germline variants associated with inherited disorders. Data Sources.-The approaches used by 2 clinical laboratories for the development and validation of targeted NGS gene panels are described. Important design and validation considerations are examined. Conclusions.-Clinical laboratories must validate performance specifications of each test prior to implementation. Test design specifications and validation data are provided, outlining important steps in validation of targeted NGS panels by clinical diagnostic laboratories

Corticosteroids for pneumonia

BackgroundPneumonia is a common and potentially serious illness. Corticosteroids have been suggested for the treatment of different types of infection, however their role in the treatment of pneumonia remains unclear. This is an update of a review published in 2011.ObjectivesTo assess the efficacy and safety of corticosteroids in the treatment of pneumonia.Search methodsWe searched the Cochrane Acute Respiratory Infections Group's Specialised Register, CENTRAL, MEDLINE, Embase, and LILACS on 3March 2017, together with relevant conference proceedings and references of identified trials. We also searched three trials registers for ongoing and unpublished trials.Selection criteriaWe included randomised controlled trials (RCTs) that assessed systemic corticosteroid therapy, given as adjunct to antibiotic treatment, versus placebo or no corticosteroids for adults and children with pneumonia.Data collection and analysisWe used standard methodological procedures expected by Cochrane. Two review authors independently assessed risk of bias and extracted data. We contacted study authors for additional information. We estimated risk ratios (RR) with 95% confidence intervals (CI) and pooled data using the Mantel-Haenszel fixed-effect model when possible.Main resultsWe included 17 RCTs comprising a total of 2264 participants; 13 RCTs included 1954 adult participants, and four RCTs included 310 children. This update included 12 new studies, excluded one previously included study, and excluded five new trials. One trial awaits classification.All trials limited inclusion to inpatients with community-acquired pneumonia (CAP), with or without healthcare-associated pneumonia (HCAP). We assessed the risk of selection bias and attrition bias as low or unclear overall. We assessed performance bias risk as low for nine trials, unclear for one trial, and high for seven trials. We assessed reporting bias risk as low for three trials and high for the remaining 14 trials.Corticosteroids significantly reduced mortality in adults with severe pneumonia (RR 0.58, 95% CI 0.40 to 0.84; moderate-quality evidence), but not in adults with non-severe pneumonia (RR 0.95, 95% CI 0.45 to 2.00). Early clinical failure rates (defined as death from any cause, radiographic progression, or clinical instability at day 5 to 8) were significantly reduced with corticosteroids in people with severe and non-severe pneumonia (RR 0.32, 95% CI 0.15 to 0.7; and RR 0.68, 95% CI 0.56 to 0.83, respectively; high-quality evidence). Corstocosteroids reduced time to clinical cure, length of hospital and intensive care unit stays, development of respiratory failure or shock not present at pneumonia onset, and rates of pneumonia complications.Among children with bacterial pneumonia, corticosteroids reduced early clinical failure rates (defined as for adults, RR 0.41, 95% CI 0.24 to 0.70; high-quality evidence) based on two small, clinically heterogeneous trials, and reduced time to clinical cure.Hyperglycaemia was significantly more common in adults treated with corticosteroids (RR 1.72, 95% CI 1.38 to 2.14). There were no significant differences between corticosteroid-treated people and controls for other adverse events or secondary infections (RR 1.19, 95% CI 0.73 to 1.93).Authors' conclusionsCorticosteroid therapy reduced mortality and morbidity in adults with severe CAP; the number needed to treat for an additional beneficial outcome was 18 patients (95% CI 12 to 49) to prevent one death. Corticosteroid therapy reduced morbidity, but not mortality, for adults and children with non-severe CAP. Corticosteroid therapy was associated with more adverse events, especially hyperglycaemia, but the harms did not seem to outweigh the benefits

Automated multi-day tracking of marked mice for the analysis of social behaviour

A quantitative description of animal social behaviour is informative for behavioural biologists and clinicians developing drugs to treat social disorders. Social interaction in a group of animals has been difficult to measure because behaviour develops over long periods of time and requires tedious manual scoring, which is subjective and often non-reproducible. Computer-vision systems with the ability to measure complex social behaviour automatically would have a transformative impact on biology. Here, we present a method for tracking group-housed mice individually as they freely interact over multiple days. Each mouse is bleach-marked with a unique fur pattern. The patterns are automatically learned by the tracking software and used to infer identities. Trajectories are analysed to measure behaviour as it develops over days, beyond the range of acute experiments. We demonstrate how our system may be used to study the development of place preferences, associations and social relationships by tracking four mice continuously for five days. Our system enables accurate and reproducible characterisation of wild-type mouse social behaviour and paves the way for high-throughput long-term observation of the effects of genetic, pharmacological and environmental manipulations

Compact Nuclei in Galaxies at Moderate Redshift:II. Their Nature and Implications for the AGN Luminosity Function

This study explores the space density and properties of active galaxies to z=0.8. We have investigated the frequency and nature of unresolved nuclei in galaxies at moderate redshift as indicators of nuclear activity such as Active Galactic Nuclei (AGN) or starbursts. Candidates are selected by fitting imaged galaxies with multi-component models using maximum likelihood estimate techniques to determine the best model fit. We select those galaxies requiring an unresolved point-source component in the galaxy nucleus, in addition to a disk and/or bulge component, to adequately model the galaxy light. We have searched 70 WFPC2 images primarily from the Medium Deep Survey for galaxies containing compact nuclei. In our survey of 1033 galaxies, the fraction containing an unresolved nuclear component greater than 5% of the total galaxy light is 9+/-1% corrected for incompleteness. In this second of two papers in this series, we discuss the nature of the compact nuclei and their hosts. We present the upper limit luminosity function (LF) for low-luminosity AGN (LLAGN) in two redshift bins to z=0.8. Mild number density evolution is detected for nuclei at -18 -16 and this flatness, combined with the increase in number density, is inconsistent with pure luminosity evolution. Based on the amount of density evolution observed for these objects, we find that almost all present-day spiral galaxies could have hosted a LLAGN at some point in their lives. We also comment on the likely contribution of these compact nuclei to the soft X-ray background.Comment: 50 pages, 14 figures, to appear in ApJ, April 199

Boson Stars as Gravitational Lenses

We discuss boson stars as possible gravitational lenses and study the lensing effect by these objects made of scalar particles. The mass and the size of a boson star may vary from an individual Newtonian object similar to the Sun to the general relativistic size and mass of a galaxy close to its Schwarzschild radius. We assume boson stars to be transparent which allows the light to pass through them though the light is gravitationally deflected. We assume boson stars of the mass $M = 10^{10}M_\odot$ to be on non-cosmological distance from the observer. We discuss the lens equation for these stars as well as the details of magnification. We find that there are typically three images of a star but the deflection angles may vary from arcseconds to even degrees. There is one tangential critical curve (Einstein ring) and one radial critical curve for tangential and radial magnification, respectively. Moreover, the deflection angles for the light passing in the gravitational field of boson stars can be very large (even of the order of degrees) which reflects the fact they are very strong relativistic objects. We also propose a suitable formula for the lens equation for such large deflection angles, and with the reservation that large deflection angle images are highly demagnified but in the area of the tangential critical curve, their existence may help in observational detection of suitable lenses possessing characteristic features of boson stars which could also serve as a direct evidence for scalar fields in the universe.Comment: accepted by Astrophys. J., 31 pages, AASTeX, 6 figure

LNCS

In resource allocation games, selfish players share resources that are needed in order to fulfill their objectives. The cost of using a resource depends on the load on it. In the traditional setting, the players make their choices concurrently and in one-shot. That is, a strategy for a player is a subset of the resources. We introduce and study dynamic resource allocation games. In this setting, the game proceeds in phases. In each phase each player chooses one resource. A scheduler dictates the order in which the players proceed in a phase, possibly scheduling several players to proceed concurrently. The game ends when each player has collected a set of resources that fulfills his objective. The cost for each player then depends on this set as well as on the load on the resources in it – we consider both congestion and cost-sharing games. We argue that the dynamic setting is the suitable setting for many applications in practice. We study the stability of dynamic resource allocation games, where the appropriate notion of stability is that of subgame perfect equilibrium, study the inefficiency incurred due to selfish behavior, and also study problems that are particular to the dynamic setting, like constraints on the order in which resources can be chosen or the problem of finding a scheduler that achieves stability

Corneal Healing after Uncomplicated LASIK and Its Relationship to Refractive Changes: A Six-Month Prospective Confocal Study

PURPOSE. To investigate corneal healing and the factor(s) possibly responsible for refractive changes after laser in situ keratomileusis (LASIK). METHODS. Twenty eyes of 10 patients who underwent LASIK for myopia were examined clinically and by real-time confocal microscopy for 6 months. Epithelial and posterior stromal thicknesses and the thickness of the keratocyte activation zone were measured, and refractive changes were compared with these values. Keratocyte morphology, flap thickness, and subbasal nerve fiber bundle morphology after LASIK were also investigated. RESULTS. No significant change was detected over time in epithelial thickness after LASIK treatment; however, the posterior stromal thickness was found to be significantly higher 1 month after surgery. A slight but statistically significant negative correlation was detected between the thickness of the keratocyte activation zone and the spheroequivalent refraction after LASIK. The subbasal nerve fiber bundle's morphology returned to its preoperative appearance 6 months after LASIK, but in the flap stroma the nerve fiber bundle morphology remained abnormal at 6 months after LASIK surgery. CONCLUSIONS. A weak but significant negative correlation between the thickness of the keratocyte activation zone and spheroequivalent refraction was found after LASIK. The different refractive properties of activated keratocytes may be responsible for the myopic shift after LASIK. Further studies are needed to clarify this hypothesis. (Invest Ophthalmol Vis Sci. 2004;45:1334 -1339) DOI:10.1167/iovs.03-1025 L aser in situ keratomileusis (LASIK) is a relatively new technique for correction of myopia. A hinged flap (consisting of epithelium, Bowman's layer, and anterior stroma) is created first, and the exposed stroma is photoablated after the flap is folded back. Although many studies have been published on the clinical outcome after LASIK, 1-4 relatively few reports address the biological changes associated with the procedure. 10,11 Keratocyte activation was strongest at 1 to 2 weeks and persisted until 3 months after LASIK surgery. 10,13 Neither LASIK nor PRK has been shown superior in efficacy outcomes 14 -16 A recent confocal microscopic study revealed that keratocyte-mediated regrowth of the photoablated stroma was a key biological factor responsible for post-PRK refractive instability in humans treated with PRK. The purpose of this study was to investigate the factor(s) responsible for the refractive changes after LASIK. For this purpose, epithelial thickness, posterior stromal thickness, and the thickness of the keratocyte activation zone were measured by confocal microscopy, and we sought to establish a correlation between refractive changes and these measurements. We also investigated keratocyte morphology, flap thickness, and subbasal nerve fiber bundle morphology after LASIK. METHODS Design This prospective, interventional cohort study was begun after approval was obtained from the LSU Health Sciences Center institutional review board. Each patient gave written informed consent, and the research followed the tenets of the Declaration of Helsinki. Patients Twenty eyes of 10 patients who underwent LASIK for myopia were included in the study. All eyes had normal anterior ocular segments, intraocular pressure (Ͻ20 mm Hg), and fundi. Contact lens wear was discontinued 2 weeks (soft lenses) or 3 weeks (hard lenses) before the LASIK operation. There were six women and four men (mean age, 35.4 Ϯ 8.7 years). All patients were 21years of age or older and had stable refractive errors at least 1 year before the laser procedure. Patients who had undergone reoperation, those with diabetes mellitus or glaucoma, or those using any topical ophthalmic medication were excluded. Patients with corneas thinner than 500 m centrally and/or with a severe systemic disorder that could cause them to miss examinations were also excluded. The average preoperative spheroequivalent refraction was Ϫ5.87 Ϯ 3.45 D (range, Ϫ1.75-11.00 D) and the planned ablation depth was 59.8 Ϯ 27.1 m (range, 16 -110 m). Each patient was examined in the pre-and postoperative period. Preoperative examinaFrom th