Pengaruh Perlakuan Sortasi, Natrium Hipoklorit Dan Fungisida Pada Kacang Tanah Untuk Mengeliminasi Kontaminasi Aspergillus Flavus

The Effect of Sorting, Sodium hypochloride (NaClO), and Fungicide Treatment on Peanut in Elimination the Aspergillus flavus Contamination. The humid tropical condition of Indonesia, the post harvest treatment, and the storage practices favour rapid growth and wide spread Aspergillus infestation on peanut. The objectives of this research was to find the best technique in controlling the Aspergillus infection on peanut. To achieve those objectives, complete randomised design in factorial with three factors was applied. The factors were grading and colour sorting (A1 = grading and colour sorting; A2 = without grading and colour sorting), NaClO blanching (B1 = without blanching; B2 = 1.25% NaClO blanching; B3=2.5% NaClO blanching), and Benlate T-20 WP treatment (C1 = 2,5 g/L; C2 = 3 g/L; C3 = 3,5 g/L). The result showed that the best combination treatment was A2B2C3 with the minimum amount of spore on peanut seed. With those combination treatment we concluded that the grading and colour sorting, the 1.25% NaClO blanching, and the application of 3,5 g/L Benlate T-20 WP could reduced the Aspergillus contamination up to 80% compare with A1B1C3 treatment

Regenerasi Embriogenesis Somatik pada Beberapa Klon Kakao Indonesia dari Eksplan Bunga

<!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:IN; mso-no-proof:yes;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> This research was aimed to observe the response of different clones and specifi  c organs due to the somatic embryogenesis regeneration. It was arranged in factorial randomized completely design with three replication. The fi  rst factor was cocoa clones i.e. ICCRI 01, ICCRI 02, ICCRI 03, ICCRI 04, KW 514, RCC72, and Sca 6. The second factor was fl  ower parts i.e. petal, staminode and anther. Every explant was regenerated on initiation, induction, multiplication and rooting media. Almost all treatments showed high response of embryogenic calli which range 89.5 to 100% at initial stage, but different results were found at the following process of somatic embryogenesis. The experiment showed that each clones and each different part of fl  ower had different response to somatic embryogenesis. The highest response of the explant number resulted from Sca 6 clone, which produce 35.8% embryo with average number of embryo per explant (1.34) followed by RCC 72 (28.4%, averaged 0.7) ICCRI 03 (24.7%, averaged 1.3) ICCRI 04 (18.6%, averaged 0.6). While ICCRI 02 showed the lowest responsive clone. Especially for ICCRI 01, 55.8% explant was rooted and  only 1.3% explant producing embryo. The highest response of somatic embryo was resulted form petal

Efektivitas Gen CP PSTV dalam Memproteksi Nicotiana Benthamiana Transgenik TO terhadap Serangan Peanut Stripe Virus

The aims of this research were: (1) to obtain the transgenic Nicotiana benthamiana T0 which have various cp Peanut stripe virus (PStV) genes construct (pBINRCP 1, pBINRCP2, pBINRCP3, and pBINRCP4); (2) to investigate the resistance of transgenic N. benthamianaT0 toward PStV infection; (3) to investigate the effectiveness of those construct to protect N. benthamiana T0 toward PStV infection. To achieve those objectives N. benthamiana T0 (70 plants) were regenerated, PCR tested and infected with PStV using biological analysis methods. The result showed that all of PStV construct gave the resistancy toward PStV infection. The phenotypic respon of those plants were the PBINRCPl plants 35.7% resistance, 0% recovery, and 64.3% susceptible, the PBINRCP2 plants 41.7%, resistance, 0% recovery, and 58,3% susceptible, the PBINRCP3 plants 71.4% resistance, 0% recovery, and 28.6% susceptible, and the PBINRCP4 plants 25.0% resistance, 25.0% recovery, and 50.0% susceptible