Additional file 1: Figure S1. of Effect of the synthetic cannabinoid HU-210 on quorum sensing and on the production of quorum sensing-mediated virulence factors by Vibrio harveyi

Comparison of relative bioluminescence production by V. harveyi MM77 (AI-1-, AI-2-, sensor-1+, sensor-2+) with different HU-210 concentrations when simultaneously supplemented with exogenous AI-1 and AI-2 from V. harveyi mutant strains MM30 (AI-1+, AI-2-) and BB152 (AI-1-, AI-2+) respectively, presented as area under the curve. Presented data are means and SD of three independent experiments, each performed in triplicate. *P < 0.05. Figure S2. Biofilm biomass quantification of V. harveyi wild type BB120, mutant strain MM30 and BB152 biofilms using CV staining. The staining strength is an indication of the amount of biofilm mass formed in AB –media with/without different concentrations of HU-210. Graph represents calculation of biofilm biomass relatively with control. Presented data are means and SD of three independent experiments, each performed in triplicate. *P < 0.05 (DOC 827 kb

Additional file 1: Figure S1. of Effect of the synthetic cannabinoid HU-210 on quorum sensing and on the production of quorum sensing-mediated virulence factors by Vibrio harveyi

Comparison of relative bioluminescence production by V. harveyi MM77 (AI-1-, AI-2-, sensor-1+, sensor-2+) with different HU-210 concentrations when simultaneously supplemented with exogenous AI-1 and AI-2 from V. harveyi mutant strains MM30 (AI-1+, AI-2-) and BB152 (AI-1-, AI-2+) respectively, presented as area under the curve. Presented data are means and SD of three independent experiments, each performed in triplicate. *P < 0.05. Figure S2. Biofilm biomass quantification of V. harveyi wild type BB120, mutant strain MM30 and BB152 biofilms using CV staining. The staining strength is an indication of the amount of biofilm mass formed in AB –media with/without different concentrations of HU-210. Graph represents calculation of biofilm biomass relatively with control. Presented data are means and SD of three independent experiments, each performed in triplicate. *P < 0.05 (DOC 827 kb

Reagents and conditions: (a) SeO₂, t-BuOOH, CH₂Cl₂, r.t; (b) DAST, CH₂Cl₂, 0°C.

<p>Reagents and conditions: (a) SeO₂, t-BuOOH, CH₂Cl₂, r.t; (b) DAST, CH₂Cl₂, 0°C.</p

Lack of effect of 3 (10 and 30 mg/kg, n = 7–8 animals/group) in Swiss male mice tested in the pre-pulse inhibition model.

<p>Animals received a first injection of vehicle (V) or <b>3</b> followed, 30 min later, by vehicle (n = 7) or amphetamine 10 mg/kg (V+amphetamine group = 7 animals). Data represents the means+SEM. *indicates difference from V+V group (ANOVA followed by the Duncan test, p<0.05).</p

Effect of 1 (1, 3 and 10 mg/kg, n = 7, 6 and 15 animals, respectively) in Swiss male mice tested in the pre-pulse inhibition model.

<p>Animals received a first injection of vehicle (V) or <b>1</b> followed, 30 min later, by vehicle (n = 9) or amphetamine 10mg/kg (V+amphetamine group = 11 animals). *indicates difference from V+V group. + indicates difference from V+amphetamine group (ANOVA followed by the Duncan test, p<0.05).</p

Reagents and conditions: (a) 1-fluoropyridinium triflate, CH₂Cl₂, r.t.

<p>Reagents and conditions: (a) 1-fluoropyridinium triflate, CH₂Cl₂, r.t.</p

Effect of 3 (1, 3 and 10 mg/kg, n = 5, 5 and 6 animals, respectively) in Swiss male mice tested in the forced swim test (FST, upper panel) and elevated plus maze (EPM).

<p>*indicates difference from V group (ANOVA followed by the Duncan test, p<0.05). Further specification as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158779#pone.0158779.g004" target="_blank">Fig 4</a>.</p

Lack of effect of 5a (3, 10 and 30 mg/kg, n = 8 animals/group) in Swiss male mice tested in the pre-pulse inhibition model.

<p>Animals received a first injection of vehicle (V) or <b>5a</b> followed, 30 min later, by vehicle or amphetamine 10 mg/kg (V+amphetamine). *indicates difference from V+V group (ANOVA followed by the Duncan test, p<0.05). Further specifications as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158779#pone.0158779.g007" target="_blank">Fig 7</a>.</p

Lack of effect of 2 (10, 30 and 60 mg/kg, n = 7–8 animals/group) in Swiss male mice tested in the pre-pulse inhibition model.

<p>Animals received a first injection of vehicle (V) or 2 followed, 30 min later, by vehicle (n = 12) or amphetamine 10 mg/kg (V+amphetamine group = 11 animals). *indicates difference from V+V group (ANOVA followed by the Duncan test, p<0.05).</p

CBD (30–60 mg/kg, n = 10–11 animals/group), HUF-101 (10 mg/kg, n = 13–14 animals/group) and fluoxetine (10 mg/kg, n = 10 animals) decreased the number of buried marbles in the MBT test.

<p>*indicates difference from V+V group (ANOVA followed by the Duncan test, p<0.05).</p