COMPARATIVE STUDY ON HYDROLYTIC ENZYMES PRODUCED BY DIFFERENT MORPHOLOGICAL FORMS OF CANDIDA ALBICANS

In recent years, the incidence of fungal infections has been rising all over the world. The ability of Candida albicans to switch from yeast to hyphal growth is essential for its virulence. The aim of this comparative study was to biotype and characterize phospholipase, proteinase, phosphatase and haemolytic activities of yeast and hyphal forms of Candida albicans. The hyphal form of Candida albicans secrets high quantity of hydrolytic enzymes than yeast form, which helps in its virulence. These results suggest that pathogenic fungi produce larger amount of inducible hydrolytic enzymes than non-pathogenic fungi. In this investigation, plate methods were used to determine the phospholipase, proteinase and haemolytic activities and spectrophotometric method was employed for testing acid phosphatase activity

Photo-Induced Cytotoxicity and Anti-Metastatic Activity of Ruthenium(II)-Polypyridyl Complexes Functionalized with Tyrosine or Tryptophan

The synergistic effect of oxygen, light, and photosensitizer (PS) has found applications in medicine for the treatment of cancer through photodynamic therapy (PDT). Induction of apoptosis to cancerous cells will prevent tumor metastasis that spreads cancer cells to the neighboring organs/tissues. Herein, we report the two apoptotic Ru(II)–polypyridyl complexes that are functionalized with pendant amino acid moieties tyrosine (1) and tryptophan (2), respectively. These two water soluble complexes were found to interact strongly (K1a = (1.18 ± 0.28) × 105 M−1 and K2a = (1.57 ± 0.77) × 105 M−1) with CT-DNA. Isothermal titration calorimetry (ITC) studies revealed that these complexes bind to CT-DNA through an entropically driven process. Both the complexes showed photo-induced cytotoxicity and exhibit apoptotic activity under photo-irradiation conditions. The comet assay indicated that these complexes can damage cellular DNA, which is attributed to the significant build-up of 1O2 level even on irradiation with low intensity light (10 J cm−2, λRange 450–480 nm). This photoinduced DNA damage and apoptosis in A549 cells was induced by reactive oxygen species (ROS) and occurred through up-regulation of apoptotic marker caspase-3. Control experiments under dark conditions revealed an insignificant cytotoxicity towards these cells for two photosensitive molecules

Data for: Comparison of Approximation-assisted Heat Exchanger Models for Steady-state Simulation of Vapor Compression System

This spreadsheet contains the data used to create the plots in the accompanying manuscript. It also provides the detailed algorithm parameters used for Kriging metamodels, to facilitate repeatability of the results presented in the manuscript.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

DEVELOPMENT OF A NUMERICAL TOOL FOR DYNAMIC SIMULATIONS OF TWO-PHASE COOLING SYSTEMS

In this article, the development of a simulation tool for two-phase cooling systems is discussed. The targeted application is modelling of the two-phase pumped loop systems used for Silicon tracking detector cooling at CERN. These systems are similar to vapour compression systems in that the thermal dynamics of such systems are dictated by the two-phase fluid present inside the heat exchangers. To properly account for such dynamics, non-homogenous void fraction based two-phase flow models (used for accurate modelling of vapour compression systems) have been incorporated. The tool has been validated against measurements taken for an R-410A-based residential heat pump unit. Both the heating and cooling mode have been simulated and the results have been compared against measured data. The simulated transients are found to compare well against measured trends. The simulations proceed faster than real-time. The tool shows readiness for use in the design of future detector cooling systems