Migration patterns of dendritic cells in the mouse. Traffic from the blood, and T cell-dependent and -independent entry to lymphoid tissues.

Dendritic cells (DC) are critical accessory cells for primary immune responses and they may be important stimulators of transplantation reactions, but little is known of their traffic into the tissues. We have studied the migration of purified splenic DC and T lymphocytes, labeled with 111Indium-tropolone, in syngeneic and allogeneic mice. First we demonstrate that DC can migrate from the blood into some lymphoid and nonlymphoid tissues. Immediately after intravenous administration, radio-labeled DC were sequestered in the lungs, but they actively migrated into the liver and spleen and reached equilibrium levels between 3 and 24 h after transfer. At least half of the radiolabel accumulated in the liver, but the spleen was the principal site of DC localization in terms of specific activity (radiolabel per weight of tissue). DC were unable to enter Peyer's patches, or mesenteric and other peripheral lymph nodes from the bloodstream. This was also true in splenectomized recipients, where the otherwise spleen-seeking DC were quantitatively diverted to the liver. In contrast, T cells homed readily to the spleen and lymph nodes of normal mice and increased numbers were present in these tissues in splenectomized mice. Thus, unlike T cells, DC cannot recirculate from blood to lymph via the nodes. We then show that migration of DC from the blood into the spleen is dependent on the presence of T cells: DC did not enter the spleens of nude mice, but when they were reconstituted with T cells the numbers entering the spleen resembled those in euthymic mice. In nude mice, as in splenectomized recipients, the DC that would normally enter the spleen were quantitatively diverted to the liver. These findings suggest that there is a spleen-liver equilibrium for DC, that may be akin to that existing between spleen and lymph node for T cells. Finally, we followed the traffic of radiolabeled DC via the afferent lymphatics after subcutaneous footpad inoculation. DC accumulated in the popliteal nodes but did not migrate further to the inguinal nodes. There was no difference between euthymic and nude mice, showing that unlike traffic to the spleen, this route probably does not require T cells. These migration patterns were not affected by major histocompatibility barriers, and were only seen with viable, but not glutaraldehyde-fixed, DC.(ABSTRACT TRUNCATED AT 400 WORDS

Migration patterns of dendritic cells in the mouse. Homing to T cell-dependent areas of spleen, and binding within marginal zone.

Using quantitative techniques we have shown elsewhere that dendritic cells (DC) migrate from blood into the spleen, under the control of T cells. Here we traced the localization of DC within the spleen and sought to explain the means by which they entered. DC were labeled with a fluorochrome, Hoescht 33342, and injected intravenously. Spleens were removed 3 or 24 h later and DC were visualized within particular areas that were defined by mAbs and FITC anti-Igs. At 3 h most DC were in the red pulp, whereas by 24 h the majority had homed to T-dependent areas of the white pulp and may have become interdigitating cells. Lymphoid DC, isolated from spleen and perhaps normally present in blood, may thus be a migratory stage distinct from the relatively fixed interdigitating cells. We also developed a frozen section assay to investigate the interaction of DC with various lymphoid elements. When DC were incubated on sections of spleen, at 37 degrees C but not at 4 degrees C they attached specifically within the marginal zone and did not bind to T areas; in contrast, macrophages attached only to red pulp and T cells did not bind specifically. However, DC did not bind to sections of mesenteric lymph node, whereas T cells localized in particular regions at 4 degrees C but not at 37 degrees C, probably the high endothelial venules. DC may thus express "homing receptors," similar to those of T cells, for certain endothelia. We propose that T cells can modify the vascular endothelium in certain areas to allow egress of DC from the bloodstream

Best Practices: Encouraging the Modality Between Melee and Ranged Combat Styles

This thesis aims to determine what gameplay factors encourage and discourage players from switching between melee and ranged playstyles while in combat scenarios. The practices for the artifact were found in action-focused video games and then incorporated into a standalone level in a Dying Light mod

Examining Cultural Influences on Dietary Behaviors among Mexican Americans with Low Acculturation

Mexican Americans represent approximately 63 percent of the total Hispanic population in the US and are disproportionately affected by health disparities related to poor nutrition status. With this community among the fastest growing populations in the US, it is critical to address the health disparities and the relationship between culture and diet to provide evidence-based nutrition interventions that are culturally sensitive to specific communities. The purpose of this cross-sectional exploratory study is to examine the relationship between cultural values and dietary behaviors among a low-income, urban, clinical sample of Mexican Americans using the Mexican American Cultural Values Scale, dietary assessment, anthropometric data, and medical records. The study findings showed a significant positive association between religion cultural values and nopal use for health reasons, as well as a significant positive association between mainstream cultural values and vegetable use for health reasons. Implications for future research include integration of these health beliefs into nutrition interventions and further examination of how health beliefs moderate or mediate the relationship between cultural values and dietary behaviors

The perfection can be a non-coherent GCD domain

We show that there exists a complete local Noetherian normal domain of prime characteristic whose perfection is a non-coherent GCD domain, answering a question of Patankar in the negative concerning characterizations of $F$-coherent rings. This recovers and extends a result of Glaz using tight closure methods.Comment: 6 pages, comments welcome. To appear in J. Commut. Algebr

Bio Roll-Up: Self-Assembling Scaffolds For Tissue Engineering

There is an unmet need for controlled, dynamic cell scaffolds that will generate 3-D monolayers of epithelial cells for medical research and tissue engineering. Here we describe a system that uses photoimageable and biocompatible HEMA-based hydrogel bilayers that self-assemble from 2-D substrates into 3-D tube-shaped structures. These structures are patterned on top of another layer that demonstrates an ability to anchor the assembled structure and control the direction and extent of selfassembly. Cell viability was determined by MTT assay which shows material components comparable with the positive control (80-110%). Finally, the masks used in the experiments herein were redesigned to improve ease of use, alignment and testability

PREFERRED, NOT ASSURED: ANTICIPATING U.S.–JAPAN COOPERATION IN A TAIWAN CONTINGENCY

With the dramatic rise in the PRC's economic and military power, concerns are mounting over the possibility of conflict over Taiwan, which could bring the United States and PRC into direct combat. The ability of the U.S. to effectively provide support to Taiwan in the event of conflict will depend on the degree of support provided by Japan to the U.S.–Japan alliance. Commensurate with the PRC's more assertive behavior since 2012, Japan has committed to efforts to modernize and expand the Japan Self-Defense Forces while, for the first time, vocally expressing its interest in peaceful resolution of the Taiwan issue. This thesis seeks to answer a key question: How has Japan’s defense policy adapted to the greater threat towards Taiwan and to what degree could Japan aid a U.S. military response to defend Taiwan? This thesis reviews national policy documents plus economic and survey data to trace Japan’s policy towards Taiwan and the use of military force over the previous decade. It finds that Japan has become more vocal in expressing its desire to maintain the Taiwan status quo, but that Japan is stymied in its ability to respond to a Taiwan contingency by its constitution, public preferences, and realized military capabilities. By better understanding the existing restrictions on the use of the Japan Self-Defense Force, the U.S. can more accurately include Japan in contingency planning for a Taiwan conflict.Approved for public release. Distribution is unlimited.Lieutenant, United States Nav

First year Experience: Create an Ideal College Campus

First Year Experience 101 Assignment: Working in partners, design your ideal college campusbased on six areas of college life discussed this term

Advocating for English Language Learners: The Role of the Literacy Educator

The authors explore how place-based advocacy effects and pedagogies have provided a way to address the needs of students. Insights on the ways authors have confronted challenges in their elementary and secondary settings reveal strategies that can be carefully integrated by literacy educators into other “places” of learning. TRANSLATE with x English Arabic Hebrew Polish Bulgarian Hindi Portuguese Catalan Hmong Daw Romanian Chinese Simplified Hungarian Russian Chinese Traditional Indonesian Slovak Czech Italian Slovenian Danish Japanese Spanish Dutch Klingon Swedish English Korean Thai Estonian Latvian Turkish Finnish Lithuanian Ukrainian French Malay Urdu German Maltese Vietnamese Greek Norwegian Welsh Haitian Creole Persian TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster Portal Bac