3 research outputs found

    Right Ventricle Free Wall Longitudinal Strain in Cancer Patients Following Chemotherapy

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    Introduction: The survival rate of cancer patients was high due to chemotherapy, but it can cause cardiotoxicity and increase morbidity and mortality. Most studies on cancer therapy-related cardiac dysfunction (CTRCD) focus more on the left ventricle. This study assesses chemotherapy's effect on the right ventricle, namely the right ventricle free wall longitudinal strain (RVFWLS). Methods: This study is a prospective cohort conducted from January to December 2022 on thirty-four cancer patients undergoing chemotherapy at Dr Wahidin Sudirohusodo Hospital Makassar. The RVFWLS was assessed before and after chemotherapy using echocardiographic. Result: The RVFWLS value after one cycle chemotherapy decreased compared pre-chemotherapy (4.40±4.84; p=0.001). Conclusion: The effect of chemotherapy is a decrease the RVFWLS value after the first cycle of chemotherapy. It should be considered for echocardiographic evaluation, especially the RVFWLS value every cycle of chemotherapy

    Global Longitudinal Strain and Global Circumferential Strain on Echo Heart Failure Score as 60-days Readmission Predictor in Congestive Heart Failure Patients with Left Ventricle Systolic Dysfunction in Makassar City

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    Introduction: The readmission rate due to heart failure increases every year in South Sulawesi. A Frequently used method for assessing readmission is an echocardiographic examination by measuring ejection fraction. However, recent studies show that the speckle tracking parameters are more sensitive to predict readmission of heart failure patients. In this study, we assess the role of Global Longitudinal Strain (GLS) and Global Circumferential Strain (GCS) parameters on the Echo Heart Failure Score (EHFS) as a 60-days readmission predictor in heart failure patients. Methods: We included 175 patients admitted through the inpatient installation of Integrated Heart Center Dr. Wahidin Sudirohusodo General Hospital. In addition, we reviewed up to 60 days after outpatient prospectively by collecting data through medical records. The correlation between echocardiographic parameters and readmissions was analyzed using the Spearman Rank Correlation. To determine each echocardiographic parameter's cut-off point, sensitivity, and specificity, we use Receiver Operating Characteristic (ROC) curve. Results:GLS and GCS are very sensitive and specific parameters in predicting 60-days readmission with an area under the curve (AUC) value > 0.7. This study shows that adding GLS and GCS parameters to the EHFS increases the predicted value (AUC 0.850 vs. 0.820) and sensitivity to 79%. Conclusion: GLS and GCS parameters additional on EHFS can help predict 60-days readmission of heart failure patients with sensitivity and specificity of 79% and 83%, respectively. The present study shows that with higher GLS and GCS scores on the EHFS, the risk of readmission in heart failure patients will increase

    PENGARUH HYPOXIC PRECONDITIONING TERHADAP EKSPRESI HEAT SHOCK PROTEIN (HSP) 27, HSP 70, DAN HSP 90 INTRASELULLAR PADA KULTUR ADIPOCYTE-DERIVED MESENCHYMAL STEM CELLS (AMSCs)

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    Harapan terbaru terapi infark miokard yakni memanfaatkan kemampuan multipoten stem cells yang ditransplantasikan untuk reparasi dan regenerasi jaringan miokard. Tantangan terbesar yang dihadapi yakni rendahnya stem cell survival paska ditransplantasikan pada microenvironment pro-apoptotik pada area paska infark. Heat shock protein (HSP) merupakan protein intraselullar yang berfungsi sebagai chaperone serta dapat bersifat sitoproteksi, mengurangi stress oksidatif dan mencegah apoptosis. Teknik hypoxic preconditioning pada Adipocyte-derived Mesenchymal Stem Cells (AMSCs) diharapkan meningkatkan potensi survival cell tersebut dengan cara menginduksi peningkatan Heat Shock Protein (HSP). Oleh karena itu, peneliti ingin meninjau lebih lanjut peranan paparan hypoxic preconditioning (konsentrasi O2 1%) selama 24 jam pada AMSCs untuk meneliti ekspresi HSP 27 sebagai chaperone ATP independent, serta HSP 70 dan HSP 90 sebagai chaperone ATP independent secara in vitro. Tujuan : Untuk menganalisis ekspresi HSP 27, HSP 70 dan HSP 90 intraselullar pada AMSCc yang diberi perlakuan HPC (O2 1%) dibandingkan dengan normoxia (O2 21%) secara in vitro. Metode : Penelitian ini merupakan true experimental randomized post-test design study. Sel AMSCs diisolasi dari jaringan adiposa dan dikultur hingga pasase 4. Sampel kemudian dibagi menjadi 2 kelompok yaitu kelompok hypoxia (O2 1%) dan kelompok nomoxia (O2 21%). Karakteristik AMSCs dinilai secara imunositokimia dan flowcytometry pada CD 90+, CD 105+ dan CD45-. Metode immunocytofluoresence digunakan untuk melihat ekspresi HSP 27, HSP 70 dan HSP 90. Analisis data dilakukan dengan menggunakan uji T 2 sampel bebas dan tes Mann Whitney U. Hasil : Immunocytofluoresence pada ekspresi HSP 27, HSP 70 dan HSP 90 menunjukkan peningkatan yang signifikan kelompok hypoxia dibandingkan dengan normoxia (272,00 ± 92,225 vs 86,19 ± 26,362 p<0.0001; 130,88 ± 45,416 vs 120,75 ± 97,113 p<0.05; 165,75 ± 58,930 vs 96,81 ± 22,578). Ekspresi HSP 27 meningkat lebih tinggi dibandingkan dengan HSP 70 dan HSP 90. Hal ini diassumsikan oleh karena peran HSP 27 sebagai chaperone ATP independent dibandingkan dengan HSP 70 dan 90 yang merupakan chaperone ATP dependent. Pemeriksaan imunositokimia dan flowcytometry menunjukkan ekspresi positif CD 90+, CD 105+ dan ekspresi negtif CD45-. Kesimpulan : Hypoxic preconditioning pada kultur AMSCs secara signifikan meningkatkan ekspresi HSP 27, HSP 70 dan HSP 90
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