FILMES BIODEGRADÁVEIS DE AMIDO DE MANDIOCA, PULULANA E CELULOSE BACTERIANA

The largest consumption of plastics in the world is referred to the synthetic polymers, which are not biodegradable and have a non-renewable source, generating a large environmental impact, especially in urban centers. As a result, in the last two decades several polymers obtained from renewable sources (biopolymers) have been studied as potential raw materials for the production of new biodegradable materials with different applications. The objectives of this study were to produce biodegradable films based on cassava starch, pullulan and bacterial cellulose, and also to characterize these films according to their microstructure, barrier, thermal and mechanical properties. The addition of bacterial cellulose and pullulan to the starch films resulted in films with more homogeneous surfaces, and also decreased solubility and water vapor permeability, and increased elongation and thermal stability

Estudo Farmacognóstico de Flores de Tagetes patula L. (Asteraceae)

Made available in DSpace on 2017-06-01T19:19:17Z (GMT). No. of bitstreams: 2 license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) 3.pdf: 155285 bytes, checksum: ba8a282bea5f93f3c1a3db52988af75a (MD5) Previous issue date: 29Universidade Estadual de Maringá. Programa de Pós-Graduação em Ciências Farmacêuticas. Maringá, PR, Brasil.Universidade Estadual de Maringá. Programa de Pós-Graduação em Ciências Farmacêuticas. Maringá, PR, Brasil.Universidade Estadual de Maringá. Programa de Pós-Graduação em Ciências Farmacêuticas. Maringá, PR, Brasil.Universidade Estadual de Maringá. Departamento de Farmácia. Maringá, PR, Brasil.Universidade Estadual de Londrina. Departamento de Ciências Farmacêuticas. Centro de Ciências da Saúde. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento de Agronomia. Centro de Ciências Agrárias. Londrina, PR, Brasil.Universidade Estadual de Maringá. Programa de Pós-Graduação em Ciências Farmacêuticas. Maringá, PR, Brasil.O estudo foi conduzido para o desenvolvimento de parâmetros farmacognósticos de flores de Tagetes patula L. As avaliações foram realizadas de acordo com a Farmacopeia Brasileira e/ou literatura especializada. Testes colorimétricos detectaram a presença de flavonoides, taninos e fenólicos simples. Por meio de técnicas cromatográficas: cromatografia em camada delgada (CCD) e cromatografia líquida de alta eficiência (CLAE) foi possível desenvolver um perfil cromatográfico adequado e estabelecer a presença das substâncias quercetina e rutina. Os resultados para a perda por dessecação, teor de cinzas totais, teor de extrativos, teor de resíduo seco e teor em flavonoides totais foram de: 9,35%, 5,50%, 39,54%, 46,61% e 5,24% respectivamente. No ensaio por hidro-destilação, o rendimento do óleo essencial nas flores foi de 0,15%. Estes dados fornecem informações importantes para a correta identificação e padronização de flores de T. patula.The current study was conducted to develop the pharmacognostic standards for Tagetes patula flowers. These evaluations were performed according to the Brazilian Pharmacopoeia and/or literature specialized. Colorimetric tests detected the presence flavonoids, tannins and simple phenolics. By thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) it was possible to develop a fingerprint suitable and correlating the presence of chemical substances, quercetin and rutin. The results for loss on drying, total ash content, extractives content, total solids content and total flavonoid content were: 9.35%, 5.50%, 39.54%, 46.61%, and 5.81%, respectively. The yield of essential oil in flowers was 0.15%. This study provides important information for correct identification and standardization of flowers of T. patula

Sulfonated (1 → 6)-β‑d‑Glucan (Lasiodiplodan): A Promising Candidate against the Acyclovir-Resistant Herpes Simplex Virus Type 1 (HSV-1) Strain

Herpes simplex virus type 1 (HSV-1) is a persistent human pathogen, and the emergence of strains resistant to Acyclovir (ACV, reference drug) shows the urgency to develop new treatments. We report the antiherpetic mechanism of the action of lasiodiplodan (LAS-N, (1 → 6)-β-d-glucan) and its sulfonated derivative (LAS-S3) in vitro and in vivo. LAS-S3 showed anti-HSV-1 action with high selectivity indices for HSV-1 KOS (88.1) and AR (189.2), sensitive and resistant to ACV, respectively. LAS-S3 inhibited >80% of HSV-1 infection in different treatment protocols (virucidal, adsorption inhibition, and post-adsorption effects), even at low doses, and showed a preventive effect and DNA and protein synthesis inhibition. The antiherpetic effect was confirmed in vivo by the cosmetic LAS-S3-CRÈME decreasing cutaneous lesions of HSV-1, including the AR strain. LAS-S3 possessed a broad-spectrum mechanism of action acting in the early and post-adsorption stages of HSV-1 infection, and LAS-S3-CRÈME is a potential antiherpetic candidate for patients infected by HSV-1-resistant strains