Application of biomacromolecules encapsulation systems for the long-term storage of Lactobacillus plantarum F1 and Lactobacillus reuteri 182

The aim of this study was to improve the viability of lactic acid bacteria (LAB) during extended storage of 1 year and mechanical characteristics of the calcium alginate beads with co-encapsulation of prebiotics and chitosan coating and subsequent freeze drying. The results revealed that the addition of trehalose to alginate matrix effectively protects the LAB cells during freeze drying, i.e., the survival rate has increased up to more than 92.5 %. Chitosan coating reinforced Ca-alginate beads, therefore the sphericity and mechanical strength of the beads improved. The findings also showed that bacteria encapsulation with the prebiotics resulted in more cells stability during the prolonged storage of 1 year and were 4.82 ± 0.06 log CFU g−1 in the lyophilized alginate-trehalose beads for Lactobacillus plantarum and 5.64 ± 0.08 log CFU g−1 in the lyophilized alginate-trehalose-inulin beads for Lactobacillus reuteri. No survival, however, was noted for the LAB cells in wet capsules after the same period. This study demonstrated that prebiotics had a significant impact on the viability of cells during freeze drying and storage. What is more, physical properties of the alginate beads were enhanced by coating beads with the chitosan

Bigel Matrix Loaded with Probiotic Bacteria and Prebiotic Dietary Fibers from Berry Pomace Suitable for the Development of Probiotic Butter Spread Product

This study presents a novel approach to developing a probiotic butter spread product. We evaluated the prebiotic activity of soluble dietary fibers extracted from cranberry and sea buckthorn berry pomace with different probiotic strains (Limosilactobacillus reuteri, Lacticaseibacillus paracasei, and Lactiplantibacillus plantarum), uploaded selected compatible combination in the bigel matrix, and applied it in the probiotic butter spread formulation. Bigels and products were characterized by physical stability, rheological, textural properties, and viability of probiotics during storage at different conditions. The highest prebiotic activity score was observed in soluble cranberry (1.214 ± 0.029) and sea buckthorn (1.035 ± 0.009) fibers when cultivated with L. reuteri. The bigels loaded with probiotics and prebiotic fiber exhibited a significant increase in viscosity (higher consistency coefficient 40–45 Pa·sn) and better probiotic viability (>6 log CFU/g) during long-term storage at +4 °C temperature, surpassing the bigels loaded with probiotics alone. Bigels stored at a lower temperature (−18 °C) maintained high bacterial viability (above 8.5 log CFU/g). The butter spread enriched with the bigel matrix was softer (7.6–14.2 N), indicating improved spreadability. The butter spread product consistently met the required 6 log CFU/g for a functional probiotic food product until 60 days of storage at +4 °C temperature. The butter stored at −18 °C remained probiotic throughout the entire storage period, confirming the protective effect of the bigel matrix. The study’s results showed the potential of the bigel to co-encapsulate, protect, and deliver probiotics during prolonged storage under different conditions

Fiber-Rich Cranberry Pomace as Food Ingredient with Functional Activity for Yogurt Production

In this study, different amounts (from 2% to 4.5%) of dietary fiber-rich cranberry pomace (CP) were added to yogurt before or after fermentation to increase dietary fiber content without changing the textural properties of the product. The addition of CP reduced whey loss, improved the firmness and viscosity, increased the total phenol compound content and the antioxidant capacity values (DPPH•, ABTS, and ORAC) of the yogurt in a dose-dependent manner, and had no significant effect on the viability of the yogurt culture bacteria. For all CP-supplemented yogurt samples, the bioaccessibility index of the polyphenols after in vitro intestinal phase digestion was approximately 90%. However, yogurt with CP added before fermentation exhibited a significantly (p < 0.05) lower degree of protein hydrolysis post-gastric and post-intestinal than the yogurt with CP added after fermentation. Yogurt supplemented with 4.5% CP could be considered a good antioxidant dairy product and a good source of dietary fiber