Mismatch distribution of pairwise genetic distances (<i>p</i>) of the three <i>Neocalanus</i> species, including two forms.

<p>Bar colours indicate the compared genetic distances between and within each species or form.</p

Prosome length frequency distribution for <i>Neocalanus flemingeri</i>.

<p>Individuals assigned to the small and large forms based on mitochondrial <i>COI</i> gene sequence are indicated by black and shaded bars, respectively.</p

Aligned sequences of mitochondrial <i>COI</i>, <i>12S</i>, nuclear <i>ITS</i>, and <i>28S</i> regions of the <i>Neocalanus</i> copepods.

<p>Only sites in which variations were observed in two individuals or more were included. Open box sites are the DNA regions that are “purely” diagnostic to each species. Bold letters indicate the “private” sites to each species (terminology described in Sarkar et al., 2002) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010278#pone.0010278-Sarkar1" target="_blank">[32]</a>.</p

Mean genetic distances (<i>p</i>) between the species and forms of <i>Neocalanus</i>.

<p>Distances of four genetic marker regions were estimated for the mitochondrial <i>COI</i>, <i>12S</i>, nuclear <i>ITS</i> and <i>28S</i>.</p

Sequence file for classification

Sequence file for the naϊve Bayesian classifier implemented in mothu

Raw data of morphological analysis

Morphological analysis of field-collected samples. Numbers of individuals (Inds.) and total dry weight (DW) for all species identified from each sampling location. Numbers of individuals and DW are calibrated into unsplit (1/1) samples

MOTU table for artificial community sample

MOTU list for artificial community sample (33 morphologically identified species) at the 97% threshold, with information of number of sequence reads, Blast result and representative sequence

MOTU table for field-collected samples (99% similarity)

MOTU list for field-collected samples at the 99% similarity threshold, with information of number of sequence reads in each station, and representative sequences

MOTU table for field-collected samples

MOTU list for field-collected samples at the 97% similarity threshold, with information of number of sequence reads in each station, Blast result, and representative sequences. The taxonomic order determined by the naϊve Bayesian classifier implemented in mothur is also listed

Taxonomy file for classification

Taxonomy file for the naϊve Bayesian classifier implemented in mothu