Characteristics of Sediment Transport with Alternate-Bar Migrating

Source: ICHE Conference Archive - https://mdi-de.baw.de/icheArchiv

Use of Non-Amplified RNA Samples for Microarray Analysis of Gene Expression

Demand for high quality gene expression data has driven the development of revolutionary microarray technologies. The quality of the data is affected by the performance of the microarray platform as well as how the nucleic acid targets are prepared. The most common method for target nucleic acid preparation includes in vitro transcription amplification of the sample RNA. Although this method requires a small amount of starting material and is reported to have high reproducibility, there are also technical disadvantages such as amplification bias and the long, laborious protocol. Using RNA derived from human brain, breast and colon, we demonstrate that a non-amplification method, which was previously shown to be inferior, could be transformed to a highly quantitative method with a dynamic range of five orders of magnitude. Furthermore, the correlation coefficient calculated by comparing microarray assays using non-amplified samples with qRT-PCR assays was approximately 0.9, a value much higher than when samples were prepared using amplification methods. Our results were also compared with data from various microarray platforms studied in the MicroArray Quality Control (MAQC) project. In combination with micro-columnar 3D-Gene™ microarray, this non-amplification method is applicable to a variety of genetic analyses, including biomarker screening and diagnostic tests for cancer

ブルゴーニュ ダイガク ボルドー ダイガク ニ オケル チョウサ ホウコク オヨビ コクサイ コウリュウ ニ ムケテ ノ テイゲン

本稿では、2010年夏に行ったブルゴーニュ大学およびボルドー大学の調査訪問をもとに、大学の概要や周辺環境、将来的に可能な学術交流と留学プログラムをまとめた。本学がフランスの大学と交流関係をもつことは研究・教育双方の面で必要であり大きなメリットがある。フランス語語学研修の実現および長期留学を視野に入れて、当該大学との提携が推進されるべきである。海外との大学間協力とそれを促進する体制づくりは、真に魅力ある「開かれた大学」づくりの一環として、本学が今後検討していくべき課題であろう。This report, based on a research trip to the University of BurgundyUniversité de Bourgogneand the University of Bordeaux(Université de Bordeaux) in the summer of 2010, summed up the outlines and surrounding environments of the universities, and academic exchanges and overseas study programs available in the future. Establishing a relationship between universities in France and SUAC is necessary and will be a great advantage for both study and education. Collaboration with the above-mentioned universities should be promoted with a view to enable a French language program and a long-term overseas study program. Collaboration with overseas universities and constructing an organization to promote it are the challenges SUAC should address in the future as part of developing truly fascinating "Open University"

MicroRNA-8073: Tumor suppressor and potential therapeutic treatment.

The comprehensive screening of intracellular and extracellular microRNAs was performed to identify novel tumor suppressors. We found that miR-8073 was present in exosome and predominantly exported from colorectal cancer cells. Treatment with a synthetic miR-8073 mimic resulted in a dramatic decrease in the proliferation of various types of cancer cells, which was not observed in similarly treated normal cells. As little is known about the biological functions of miR-8073, its target mRNAs were analyzed by both mRNA expression and in silico sequence analyses, leading to five probable target candidates (FOXM1, MBD3, CCND1, KLK10, and CASP2) that enhance survival during the regulation of the cell cycle, cell proliferation, and apoptosis. We experimentally confirmed that miR-8073 binds the 3'-UTR of each of these mRNA target candidates and that the introduction of a synthetic miR-8073 mimic into cancer cells reduced levels of protein expression. Finally, the antiproliferative effects of miR-8073 were validated in vivo: the subcutaneous injection of a synthetic miR-8073 mimic suppressed colorectal tumor volume to 43% in tumor-bearing xenografted mice. These results suggest that because miR-8073 binds, and thus reduces the levels of, these oncogenic targets, cancer cells must actively downregulate miR-8073 as a survival mechanism. The introduction of miR-8073 into tumors could thus inhibit tumor growth, indicating its great potential for cancer therapeutics

Spearman's rank correlation between the microarray assay and qRT-PCR assay.

<p>UHRR, HBRR, breast, and colon RNA derived from human tissue or cells were processed by the non-amplification (black bar), the 1-round amplification (grey bar), or the 2-round amplification (white bar) method, analyzed by microarray. The obtained PolR2A-normalized signal intensity (log₂) of the 42 genes (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031397#pone.0031397.s001" target="_blank">Table S1</a>) was compared with ΔCt values of the same samples analyzed by qRT-PCR assays.</p