Cardiorespiratory effects of venous lipid micro embolization in an experimental model of mediastinal shed blood reinfusion

<p>Abstract</p> <p>Background</p> <p>Retransfusion of the patient's own blood during surgery is used to reduce the need for allogenic blood transfusion. It has however been found that this blood contains lipid particles, which form emboli in different organs if the blood is retransfused on the arterial side. In this study, we tested whether retransfusion of blood containing lipid micro-particles on the venous side in a porcine model will give hemodynamic effects.</p> <p>Methods</p> <p>Seven adult pigs were used. A shed blood surrogate containing 400 ml diluted blood and 5 ml radioactive triolein was produced to generate a lipid embolic load. The shed blood surrogate was rapidly (<2 minutes) retransfused from a transfusion bag to the right atrium under general anesthesia. The animals' arterial, pulmonary, right and left atrial pressure were monitored, together with cardiac output and deadspace. At the end of the experiment, an increase in cardiac output and pulmonary pressure was pharmacologically induced to try to flush out lipid particles from the lungs.</p> <p>Results</p> <p>A more than 30-fold increase in pulmonary vascular resistance was observed, with subsequent increase in pulmonary artery pressure, and decrease in cardiac output and arterial pressure. This response was transient, but was followed by a smaller, persistent increase in pulmonary vascular resistance. Only a small portion of the infused triolein passed the lungs, and only a small fraction could be recirculated by increasing cardiac output and pulmonary pressure.</p> <p>Conclusion</p> <p>Infusion of blood containing lipid micro-emboli on the venous side leads to acute, severe hemodynamic responses that can be life threatening. Lipid particles will be trapped in the lungs, leading to persistent effects on the pulmonary vascular resistance.</p

Analysis of carotenoids in tomato paste by HPLC and OCC

WOS: 00008838120000

Behavioral correlates of direct current-coupled electrographic activity in premature infants.

To access publisher full text version of this article. Please click on the hyperlink in Additional Links fieldThe co-expression of behavioral and neural events represents a situation conducive to Hebbian-type neuroplasticity and may provide a reasonable explanation for how the amount of movement during the perinatal period contributes to neuromotor development. Direct current-coupled electrographic recordings in premature infants indicate that the majority of the electrographic activity is exhibited in a slow frequency range that is either distorted or not visible using traditional recording methods. Therefore, we provide a description of the behavioral correlates of direct current-coupled electrographic recordings in six premature human infants (3 males and 3 females; 30-34 weeks). We report, in concert with prior data, that electrographic activity and movements occur in tightly coupled discrete bouts. Surprisingly, spontaneous activity transients, which are slow, high amplitude, multiband electrographic events, typically precede startles; thereby revealing a previously unknown coupling of early neural and behavioral events in humans. Taken together, the present findings open novel venues for studying and dissecting mammalian neuromotor development

Determination of growth, bearing, yield and some quality characteristics of pistachio cultivars grafted on different rootstocks under irrigated conditions

This experimental study was carried out between the years of 1997 and 2008 to determine the best pistachio rootstock-cultivar combinations for irrigated conditions in Şanliurfa in Turkey. Four different Pistacia species (Pistacia khinjuk Stocks, Pistacia atlantica Desf., Pistacia vera L. and Pistacia terebinthus L.) were used as rootstocks and five standard pistachio cultivars (Siirt, Ohadi, Kellekoçi, Kerman and Selection 14) were grafted on them. Experimental orchard was established by 4×6 m in spacing in 1997. According to selected criteria, such as, tree vigor, bearing, yield and some quality characteristics, the best highlighted combinations were determined. P. khinjuk × Siirt cultivar and P. atlantica × Siirt combinations gave the best results for irrigated conditions in Şanliurfa

Effects of two different lipid emulsions on antioxidant status, lipid peroxidation and parenteral nutrition- related cholestasis in premature babies, a randomized-controlled study

PubMedID: 30177465Background: Olive oil-soybean oil (OO/SO) based lipid emulsions (LE) lack ?-3 PUFAs eicosapentaenoic acid –EPA and docosahexaenoic acid- DHA, which have clinical benefits on inflammatory processes. Fish oil based LEs are good sources of DHA and EPA. Fish oil, MCT, Olive oil and Soya oil (FMOS) lipid is one of the fish oil containing LEs supplemented with high levels of ?-tocopherol and lower levels of phytosterol compared to OO/SO lipid emulsions. We investigated the effects of OO/SO and FMOS lipid preparations on cholestasis, levels of antioxidant enzymes and lipid peroxidation. Methods: Preterm neonates ?32 gestational weeks age and/or ?1500 g were randomly assigned to receive either FMOS or OO/SO in the first day of life. Catalase, superoxide dismutase (SOD), glutathione peroxidase (GPx) and thiobarbituric acid reactive substances (TBARS) levels in the first day of life, 7th day of lipid use and 28th day of life were measured and cholestasis during parenteral nutrition was recorded. Results: 34 and 33 patients were in FMOS and OO/SO lipid groups respectively. Although the TBARS levels were higher in the first day of life and 7th day of LEs in OO/SO lipid group (p=0.014 and p=0.022), on the 28th day of life TBARS level was similar and SOD level was higher (p=0.014) in OO/SO group. Cholestasis was significantly lower in FMOS lipid group (0% vs. 18.2%), (p=0.011) and neonates regained birth weight earlier (p=0.006). There was no significant difference in other morbidities. Conclusions: FMOS and OO/SO lipid emulsions have similar effects on lipid peroxidation on 28th day of life and on morbidities in short term period except for cholestasis. © 2018Çukurova Üniversitesi: TTU-2015-4910The authors declare that there is no conflict of interest. The study was funded by Çukurova University , Scientific Project Department, Project Code: TTU-2015-4910. Appendix

Determination of growth, fruit set, yield and some nut quality characteristics of local and foreign almond cultivars in the irrigated conditions in GAP Region

This study was carried out to determine the adaptability of native and foreign almond cultivars in the irrigated conditions of GAP Region between the years of 1998 and 2007. Experiments were set up in three locations; twenty cultivars in Gaziantep and twenty-one cultivars in both Kahramanmaras and Şanliurfa provinces. In each experimental orchard, the following almond cultivars were tested: 101-9 (except Gaziantep location), Gülcan I (101-23), 17-4, Gülcan II (101-13), 48-1, Hacialibey (48-5), Akbadem (48-2), Nikitski, Cristomorto, Nonpareil, D. Largueta, Picantili, Dokuzoguz II (120-1), Primorski, Drake, Texas, Ferraduel, Tuono, Ferragnes, Yaltinski, Garrigues. Cultivar evaluation was done using Weighted Ranking Method (WRM) with the criteria of tree vigor, blooming time, yield and fruit characteristics. In Gaziantep, Ferraduel (takes 790 points in WRM) and Ferragnes (740); in Kahramanmaras Ferragnes (870) and Yaltinski (850); in Şanliurfa, Ferragnes (820), Gülcan II (710) and Ferraduel (690) can be recommended for those ecology, because of their relatively higher ranks

Investigation of the relationship of TNFRSF11A gene polymorphisms with breast cancer development and metastasis risk in patients with BRCA1 or BRCA2 pathogenic variants living in the Trakya region of Turkey

Modifying genes play an exclusive role in the genetic regulation of the risk of breast cancer development in women with a pathogenic variation of BRCA1 or BRCA2. Therefore, it has been suggested that TNFRSF11A, which is among those modifying genes present in breast cancer development, may have a significant role in patients with positive BRCA1 or BRCA2 variations. In our study, we investigated the probable effects of single nucleotide polymorphisms (SNPs) in the TNFRSF11A gene, such as rs4485469, rs9646629, rs34739845, rs17069904, rs 884205, rs4941129 on the risk of breast cancer in patients with BRCA1 or BRCA2 variations. A total of 23 breast cancer patients with pathogenic variations in the BRCA1 or BRCA2 genes, 28 patients with no pathogenic variations in the BRCA1 or BRCA2 genes, and 55 healthy women as a control group, were included in this study. The SNPs were determined with allelic discrimination analysis through the real-time polymerase chain reaction (qPCR) method. There was no statistically significant difference between the SNPs of the TNFRSF11A gene rs4485469, rs9646629, rs34739845, rs17069904, rs884205, rs4941129 and metastasis, estrogen receptor, progesterone receptor and CerB2 receptor positivity between patient and control group (p >0.05). However, the rs4485469 SNP was found to be borderline significant between the patient groups with and without BRCA1 or BRCA2 mutations (p = 0.059). In patients with BRCA1 or BRCA2 pathogenic variations living in the Trakya region of Turkey, we could not determine the relationship between TNFRSF11 SNPs with breast cancer risk