Gastrointestinal quality of life in patients with asymptomatic cholelithiasis after laparoscopic cholecystectomy

To assess the outcome of laparoscopic cholecystectomy for asymptomatic cholelithiasis before and after laparoscopic cholecystectomy using a specific quality of life instrument for gastrointestinal disorders in adults : The Gastrointestinal Quality of Life Index (GIQLI) was used to study the quality of life in patients before and after laparoscopic cholecystectomy : Seventy one patients completed the GIQLI questionnaire both preoperatively and after a minimum postoperative follow-up of three months. Mean preoperative score was 126.8±14.07 out of a theoretical maximum score of 144.After three months, the score had significantly improved to 136.6±9.31, close to the range for the normal population. Not only items assessing gastrointestinal symptoms but also the domains of physical, social, and emotional function improved significantly. The most marked improvements were achieved in patients with the lowest preoperative scores. Laparoscopic cholecystectomy significantly improves the quality of life in patients with cholelithiasis who are asymptomatic or have nonspecific gastrointestinal symptoms that cannot be explained by another gastrointestinal pathology.Bu çalısmada asemptomatik kolelitiyazis olgularının ameliyat öncesi ve ameliyat sonrası hayat kalitesi degerlendirilmistir. Hastaların ameliyat öncesi ve sonrası hayat kalitelerinin ölçümü gastrointestinal hayat kalitesi indeksi parametreleri kullanılarak belirlenmistir. Çalısmaya alınan 71 hasta ameliyat öncesi ve ameliyattan en az 3 ay sonra gastrointestinal hayat kalitesi indeksine göre sorgulanmıstır. Preoperatif dönemde ortalama skor 126.8±14.07, 3 ay sonra yapılan sorgulamada ise ortalama 136.6±9.31 olup normal populasyona yakın bir oranda saptanmıstır. (toplam skor 144). Gastrointestinal semptomlarının yanı sıra fiziksel, sosyal ve duygusal durumlarında da anlamlı düzelme gözlenmistir.Düsük skorlu hastalarda bu iyilesmedaha belirgindir. Asemptomatik kolelitiyazisli hastalarda baska gastrointestinal patolojilerle açıklanamayan nonspesifik semptomların laparoskopik kolesistektomiyle iyilesme gösterdigi, hastaların hayat kalitelerinde anlamlı bir artıs oldugu görülmektedir

Circulating miRNAs as potential molecular biomarker of early pregnancy in ewes

22nd Annual Conference of the European-Society-for-Domestic-Animal-Reproduction (ESDAR) -- SEP 27-29, 2018 -- Cordoba, SPAINWOS: 000445201100232European Soc Domest Anim ReprodTUBITAK [214O643]Supported by TUBITAK 214O643

Expression pattern of microRNAs in ovine endometrium during the peri-implantation

MicroRNA (miRNA), acting as the transcriptional regulator of gene expression, has been widely demonstrated to be involved in many biological functions, including embryo implantation and development. The objective of the current study was to illuminate the expression pattern of microRNAs (miRNAs) in the endometrium during the peri-implantation in ewes. Intercaruncular endometrial samples was obtained from a total of 24 ewes on days of 12 (pre-implantation, n = 4), 16 (implantation, n = 4) and 22 (post-implantation, n = 4) of pregnancy following mating, and on their corresponding days of 12 (n = 4), 16 (n = 4) and 22 (n = 4) of the estrous cycle. The miRNA profiles were examined in the endometrium by microarray technology. We detected 116 ovine specifics miRNAs in the endometrium. Of these, nineteen were differentially expressed in early pregnancy. Four miRNAs (oar-miR-370-3p, oar-miR-411b-5p, oar-miR-379-3p and oar-miR-411a-3p) that had the most differential fold change were confirmed by RT-qPCR in ovine endometrium. The differentially expressed miRNAs targeted a total of 315 genes, resulting in 39 GO terms in molecular function, 353 in biological process, and 17 in the cellular component. The construction of the PPI network of target genes established two functional modules mostly enriched in the innate immune system, toll receptor cascades in module 1, whereas genes in module 2 were associated with GMCSF-mediated signaling events, insulin pathway, and mTOR signaling pathway. Based on the results, we may imply that miRNAs modulate ovine endometrium during the peri-implantation

Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep

MicroRNA (miRNA) plays an important role in the control of gene expression and is implied in many biological functions, including embryo implantation and development. The aim was to assess plasma miRNA profiles during the peri-implantation and ascertain potential candidate miRNA markers for early pregnancy diagnosis in ovine plasma. The plasma samples were obtained from a total of 24 ewes on days 12 (pre-implantation; P12, n = 4), 16 (implantation; P16, n = 4) and 22 (post-implantation; P22, n = 4) after mating, and on their corresponding days of 12 (Pre-C; C12, n = 4), 16 (Imp-C; C16, n = 4) and 22 (Post-C; C22, n = 4) of the estrous cycle. The miRNA profiles in plasma were assessed by microarray technology. We detected the presence of 60 ovine-specific miRNAs in plasma samples. Of these miRNAs, 22 demonstrated a differential expression pattern, especially between the estrous cycle and early pregnancy, and targeted 521 genes. Two miRNAs (oar-miR-218a and oar-miR-1185-3p) were confirmed using RT-qPCR in the ovine plasma samples. Protein-protein interaction (PPI) network of target genes established six functional modules, of which modules 1 and 3 were enriched in the common GO terms, such as inflammatory response, defense response, and regulation of immune response. In contrast, module 2 was enriched in the developmental process involved in reproduction, embryo development, embryonic morphogenesis, and regulation of the developmental process. The results indicate that miRNAs profiles of plasma seemed to be modulated during the peri-implantation stage of pregnancy in ewes. Circulating miRNAs could be promising candidates for diagnosis in early ovine pregnancy

miRNAs expression during the peri-attachment period of pregnancy in the ovine endometrium

Joint Meeting of the 20th Annual Conference of the European-Society-for-Domestic-Animal-Reproduction (ESDAR) / 13th Conference of the Spanish-Association-for-Animal-Reproduction (AERA) -- OCT 27-29, 2016 -- Lisbon, PORTUGALWOS: 000392391400052European Soc Domest Anim Reprod, Spanish Assoc Anim ReprodTUBITAK [214O643]This study was funded by TUBITAK grant 214O643 to M. Kose

Suitability of two different commercial ısolation kits and phenol-chloroform ısolation method for fetal sex determination from maternal plasma in sheep

Dursun, Şükrü (Aksaray, Yazar)Koyunlarda fötal cinsiyetin belirlenmesinde kullanılan yöntemlerden biri maternal plazmadaki fötal DNA’ların Polimeraz Zincir Reaksiyonu (PZR) ile çoğaltılmasına dayanır. Ancak, maternal plazmadan izole edilen DNA miktarı ve kalitesi, kullanılan DNA izolasyon yöntemine göre önemli ölçüde değişebilmektedir. Bu çalışmada, üç farklı DNA izolasyon metoduyla koyun maternal plazmasından izole edilen DNA’ların PZR ile çoğaltılarak fötüs cinsiyetlerinin belirlenmesi amaçlandı. Bu amaçla, gebeliğin 20. haftasındaki 13 adet koyundan plazma örnekleri alındı ve doğum sonrası yavruların cinsiyetleri doğrulandı. Pozitif kontrol amacıyla iki adet koç plazması, negatif kontrol amacıyla ise iki adet gebe olmayan koyun plazması kullanıldı. Maternal plazmadan fenol-kloroform izolasyon metodu ve iki farklı ticari nükleik asit izolasyon kiti (QIAamp ve FitAmp) kullanılarak DNA izole edildi. İzole edilen DNA örneklerinden X ve Y kromozomları üzerindeki Amelogenin ve SRY (Sex-determining Region Y) genlerinin hedef bölgeleri PZR yöntemi ile çoğaltıldı. Ticari nükleik asit izolasyon kitleriyle elde edilen DNA’lardan SRY ve Amelogenin primerleri ile yapılan farklı PZR işlemlerinde fötüs cinsiyetinin belirlenmesine yönelik spesifik PZR ürünleri elde edilemedi. Diğer taraftan, fenol-kloroform izolasyon metoduyla izole edilen DNA örneklerinden 10’unda (7 erkek ve 3 dişi fötal DNA içeren örnekte) fötüs cinsiyeti doğru bir şekilde teşhis edildi (10/13). Ancak erkek fötüs taşıyan üç koyundan elde edilen DNA örneklerinden erkek cinsiyet teşhisine özgü PZR ürünleri elde edilemedi (3/13). Çalışmanın sonuçları koyunlarda maternal plazmadan DNA izolasyonunda iki farklı ticari nükleik asit izolasyon kiti kullanıldığında fötal cinsiyetin tespit edilemediğini, diğer taraftan fenol-kloroform izolasyon metodu ile fötal cinsiyet teşhisi yapılabildiğini, ancak bu metodun da hatalı sonuçlara neden olabildiğini göstermiştir.One of the methods used for determining fetal sex in sheep depends on amplification of fetal DNA in maternal plasma by polymerase chain reaction (PCR). However, quantity and quality of the DNA isolated from the maternal blood plasma can vary significantly depending on the DNA isolation method used. In this study aimed to determine fetal sex in maternal blood plasma by using three different DNA isolation methods. Plasma samples were collected from 13 ewes at 20th week of pregnancy and the genders of the fetuses were confirmed after birth. Plasma samples were collected from two rams as positive control and from two non-pregnant ewes as negative control. Free circulating DNA in maternal plasma samples was isolated by using phenol-chloroform isolation method and two different commercial DNA isolation kits (QIAamp and FitAmp). The obtained free circulating DNA was used for amplifying the target regions of X- and Ychromosome specific Amelogenin and SRY (Sex-determining Region Y) genes, respectively. Commercial isolation kits did not yield a PCR product of SRY or Amelogenin genes. On the other hand, the fetal sex correctly identified in 10 DNA samples (in three female and seven male fetal DNA including samples) isolated by using phenol-chloroform isolation method (10/13). However, in three samples including male fetal DNA fetal sex were incorrectly identified (3/13), because male sex specific PCR products were not obtained. The results of the study suggested that detection of fetal sex in sheep could not be achieved when two different commercial DNA isolation kits were used for DNA isolation from maternal plasma while phenol-chloroform isolation method was successful for this purpose, although incorrect results could also be obtained when this method was used