Determination of genetic diversity among Turkish durum wheat landraces by microsatellites

WOS:000290668200036Wheat landraces represent an important source of genetic variation that can be used for future wheat breeding program. The rich wheat landraces from Turkey have not been sufficiently analyzed genetically. For this reason, genetic diversity and relationship of the landraces must be determined. In this study, genetic diversity of 20 durum wheat landraces collected from different regions of Turkey were determined using 12 highly polymorphic microsatellite (SSR) markers. Genomic DNAs were isolated from ten accessions of all durum wheat landraces and specific regions were amplified with the polymerase chain reaction by using microsatellite markers. Polymorphic bands changed 4 to 9 per each SSR locus and the most polymorphic SSR loci were Wms 18, Wms 155, Xgwm 166 and Stm 578. The dendrogram showed that durum wheat landraces can be divided into two major groups. The coefficient of similarity among all germplasm ranged from 0.35 to 0.74. According to the estimated matrices and relationship among landraces accessions, it was determined that the 46847-47341, 31738-52841 and 31634-47923 were genetically the closest. The farthest genotypes were 52835-1680. The results showed that durum wheat landraces have high genetic variability and microsatellite DNA markers could be successfully employed for revealing the variability

Pregabalin treatment for neuropathic pain may damage intervertebral disc tissue

WOS: 000442280500109PubMed ID: 30112057The aim of the present study was to determine whether pharmaceutical preparations with pregabalin (PGB) as an active ingredient, which are widely prescribed by clinicians, exert toxic effects on human primary nucleus pulposus (NP) and annulus fibrosis (AF). Primary human cell cultures were obtained from intact (n=6) and degenerated (n=6) tissues resected from the two groups of patients. Different doses of PGB were applied to these cultures and cells were subjected to molecular analyses at 0, 24 and 48 h. Cell vitality, toxicity and proliferation were assessed using a spectrophotometer. The expression of chondroadherin (CHAD), a (member of the NP-specific protein family), hypoxia-inducible factor-1 alpha (HIF-1 alpha) and type II collagen (COL2A1) was measured using reverse transcription-quantitative polymerase chain reaction. The results revealed that cell intensity increased in a time-dependent manner and cell vitality continued in the cultures without pharmaceuticals. Cell proliferation was suppressed in the PGB-treated cultures independent from the dose and duration of application. PGB was demonstrated to suppress the expression of CHAD and HIF-1 alpha. In contrast, COL2A1 gene expression was not revealed in any experimental group. The present study utilized an in vitro model and the PGB active ingredient used herein may not be representative of clinical applications; however, the results demonstrated that PGB has a toxic effect on NP/AF cell cultures containing primary human intervertebral disc tissue. In summary, the use of pharmacological agents containing PGB may suppress the proliferation and differentiation of NP/AF cells and/or tissues, which should be considered when deciding on an appropriate treatment regime

Oxidative stress and apoptosis in electromagnetic waves exposed Zebrafish embryos and protective effects of conductive nonwoven fabric

The amount of technological products including television, radio transmitters, and mobile phone that have entered our daily life has increased in recent years. But these devices may cause adverse effects on human health. Electromagnetic shielding fabrics may limit and inhibit electromagnetic waves. Aim of our study was to evaluate electromagnetic wave blocking performance of nonwoven textile surfaces on zebratish embryos that were exposed to electromagnetic waves at specific frequencies. Oxidant-antioxidant system parameters were evaluated spectrophotometrically. The expressions of tp53 and casp3a were evaluated by RT-PCR. Results showed that electromagnetic shielding fabrics produced as conductive nonwoven textile surfaces improved oxidant-antioxidant status and 103 expression that were impaired in electromagnetic waves exposed zebrafish embryos. Also, electromagnetic shielding fabrics decreased casp3a expression responsible for the execution phase of apoptosis that increased in electromagnetic waves exposed zebrafish embryos.Marmara Universit