Molekularno otkrivanje i genomska svojstva psećeg virusa Torque teno u Turskoj

Torque teno virus (TTV) was first detected in humans, and since then it has been reported in many host species, such as monkeys, cats, pigs, seagulls and dogs. The aim of this study was to investigate the presence of Torque teno canis virus (TTCaV) in shelter dogs housed in Sivas Municipal Animal Shelter, Turkey. Faecal specimens, including diarrheic and non-diarrheic (n=202), were collected from dogs of various age groups. In total, 32.18% (65/202) of samples were found positive for TTCaV. Out of the 65 positive samples, 34.64% (44/127) samples were from adult diarrheic dogs and 26.09% (6/23) belonged to diarrheic puppies. On the other hand, 28.84% (15/52) positive samples were detected from clinically healthy dogs. Eight sequences showed close homology among themselves, however, the sequences of two samples (CANEL130 and CANEL140) were genetically distinct from other published sequences. This is the first report on the detection of TTCaV in adult dogs and puppies in Turkey, and provides evidence that TTCaV cannot be considered as the sole cause of diarrhea.Virus Torque teno (TTV) otkriven je najprije u ljudi, no otada je zabilježen i u mnogim drugim domaćinima kao što su majmuni, mačke, svinje, galebovi i psi. Cilj ovoga rada bio je istražiti prisutnost psećeg virusa Torque teno (TTCaV) u pasa u azilu Sivas Municipal Animal Shelter u Turskoj. Uzorci izmeta (n = 202), uključujući one s proljevom i bez proljeva, prikupljeni su od pasa različitih dobnih skupina. Ukupno je 32,18 % (65/202) uzoraka bilo pozitivno na TTCaV. Od 65 pozitivnih uzoraka njih 34,64 % (44/127) potjecalo je od odraslih pasa s proljevom, a 26,09 % (6/23) od štenadi s proljevom. S druge strane, 28,84 % (15/52) pozitivnih uzoraka utvrđeno je u klinički zdravih pasa. Osam je sekvencija pokazalo međusobnu blisku srodnost, no sekvencije dvaju uzoraka (CANEL130 i CANEL140) genetski su se razlikovale od drugih sekvencija. Ovo je prvi nalaz TTCaV u odraslih pasa i štenadi u Turskoj i dokazuje da se TTCaV ne može smatrati jedinim uzročnikom proljeva

A pediatric recalcitrant case of perioral dermatitis successfully treated with topical 0.03% tacrolimus alone

Dear Editor,Perioral dermatitis is a common acneiform eruption characterized byerythematous to skin-colored papular and pustular lesions locatedaround the eyes, nose, and mouth. It may affect both adults and chil-dren. Perioral dermatitis has been shown in patients as young as3 months. The etiology of the entity is clearly unknown. However,many of the patients have a history of atopic diathesis. The diagnosisis usually made based on clinical features (Kellen & Silverberg, 2017)..

The use of dermatoscopy in the diagnosis of erythema ab igne

We have read with great interest the original article by Ozturk et al, which was recently published in the Journal of Cosmetic Dermatology.1 In their retrospective multicenter study, the authors investigated demographic and clinical features of patients with ery-thema ab igne (EAI). They stated that the diagnosis of EAI was made based on the clinical history along with the appearance, and local-ization of the lesions..

A recalcitrant case of facial verruca plana successfully treated with topical calcipotriol alone

Dear Editor, Verruca plana is a benign epidermal proliferative cutaneous condition mainly caused by human papillomavirus (HPV) types 3 and 10. HPV 27, 28, 38, 41, and 49 may also rarely cause verruca plana.1 Face located plane warts usually cause important aesthetic concerns in patients and lead them to seek treatment. The management of verruca plana poses a therapeutic challenge for clinicians, as there is no single method that has been proven to be efficient for completely treating the disease..

Vitamin B12: An underestimated cause of acneiform drug eruption

Dear Editor,Acneiform drug eruption is a type of adverse drug reaction character-ized by monomorphic papulopustular lesions. There are a limited num-ber of studies focusing on vitamin B12-induced acneiform eruption(BIAE) in the relevant literature.1,2In this study, we retrospectivelyanalyzed the clinical characteristics of BIAE in a consecutive series of32 patients who were admitted to the dermatology departments ofAhi Evran University and Kozan State Hospital over the last 3 years.Collected demographic and clinical data for each patient included age,sex, history of medication, previous experience with vitamin B12,symptoms, morphology, distribution and duration of the eruption, andthe treatment used. Causality assessment between vitamin B12intakeand BIAE was mainly based on the diagnostic criteria for drug reac-tions that have been originally proposed by Naranjo et al3For theselection of the 32 patients, the inclusion criteria were as follows..

Utility of dermatoscopy in the diagnosis of acanthosis nigricans

We have read with great interest the review article by Das et al, which was recently published in the Journal of Cosmetic Dermatology.1The authors provided a comprehensive and updated review of cur-rent knowledge on acanthosis nigricans (AN) with particular empha-sis on diagnostic testing strategies. They pointed out that AN can imitate many different clinical entities, including epidermal nevus, psoriasis, fungal infections, atopic dirty neck, confluent and retic-ulated papillomatosis, Hailey-Hailey disease, and terra firma-forme dermatosis. The authors also underlined the importance of proper history, physical examination, and histopathological evaluation in the differential diagnosis.1 Here, we would like to briefly discuss the role of dermatoscopy as an adjunctive diagnostic tool that will facil-itate the diagnosis of AN..

Pemphigus and COVID-19: Critical overview of management with a focus on treatment choice

COVID-19 is a serious multisystem disease caused by severe acute respiratory syndrome coronavirus 2. Due to the COVID-19 crisis, that still keeps its impacts worldwide, numerous scheduled medical activities have been postponed and this interruption has a potential to modify the management of many cutaneous conditions including pemphigus. This narrative review aims to discuss the management of pemphigus in the era of COVID-19, considering the necessity to balance suitable pemphigus treatment with minimal risk of COVID-19-related mortality and morbidity. The data on the effect of treatments used for pemphigus on COVID-19 are limited. However, the evidence to manage patients properly is evolving and our knowledge is updated. Current expert recommendations include that patients with pemphigus should be informed clearly to avoid mismanagement and they should be monitored regularly for symptoms of COVID-19. Patients with mild disease can be managed with topical or intralesional corticosteroids, dapsone, or doxycycline. Systemic corticosteroids should be tapered to the lowest effective dose during the pandemic. Prednis(ol)one ≤10 mg/d can be continued in patients with COVID-19 while prednis(ol)one >10 mg/d may be reduced considering the activity of the disease. Conventional immunosuppressive therapies should only be discontinued in confirmed cases of COVID-19. Postponing rituximab treatment should be considered on a case by case basis. Intravenous immunoglobulin is not likely to increase the risk of infection and may be considered a safe option in patients with COVID-19. Given the psychological burden brought by COVID 19, online or face-to-face psychological support programs should be considered. © 2020 Wiley Periodicals LLC

An association of malignant eccrine poroma and venous insufficiency of lower extremity

Ekrin poroma nadir görülen ve ekrin ter bezlerinden kaynaklanan bir deri tümörüdür. Bu tip tümörler oldukça yavas büyürler ve diger deri kanserleriyle karısabilirler. Malign ekrin poroma ve venöz yetmezlik beraberligi literatürde simdiye kadar bildirilmemistir. Olgumuzda, sol bacak orta-arka kısmında variköz zeminde yerlesmis ekrin poroma kitlesi mevcuttu. Tanı histopatolojik degerlendirme ile konuldu. Lenf nodu ya da uzak organ metastazı saptanmadı. Bizler bu makalede 72 yasındaki bir bayan olguda, sol bacakta venöz yetmezlikle beraber görülen bir ekrin poroma kitlesinin basarılı cerrahi tedavisini sunmaya çalıstık. Bu birlikteligin tesadüfî olması veya venöz yetmezlik zemininde ekrin poroma gelismis olması olasılıgı aydınlatılamamıstır. Ancak bu makale ile bu çok nadir birliktelik tartısılmıstır.Eccrine poroma is a malignant tumour of eccrine sweat glands. It is a very rare, slow growing tumour and clinically resembles other skin cancers.We report a malignant eccrine poroma located on varicose surface of the posterior left leg of a 72 year-old woman. It was diagnosed by histopathological findings. Lymph nodes or distant metastases were not found. Malignant eccrine poroma with venous insufficiency has not been reported in the literature.Although we do not know how venous insufficiency effected the the development of eccrine poroma in our case we discuss this very rare association of eccrine poroma and venous insufficiency

Simultaneous Deletion of Virulence Factors and Insertion of Antigens into the Infectious Laryngotracheitis Virus Using NHEJ-CRISPR/Cas9 and Cre–Lox System for Construction of a Stable Vaccine Vector

Infectious laryngotracheitis virus (ILTV) is a promising vaccine vector due to its heterologous gene accommodation capabilities, low pathogenicity, and potential to induce cellular and humoral arms of immunity. Owing to these characteristics, different gene-deletion versions of ILTVs have been successfully deployed as a vector platform for the development of recombinant vaccines against multiple avian viruses using conventional recombination methods, which are tedious, time-demanding, and error-prone. Here, we applied a versatile, and customisable clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 accompanied with Cre–Lox system to simultaneously delete virulence factors and to insert foreign genes in the ILTV genome. Using this pipeline, we successfully deleted thymidine kinase (TK) and unique short 4 (US4) genes and inserted fusion (F) gene of the Newcastle disease virus without adversely affecting ILTV replication and expression of the F protein. Taken together, the proposed approach offers novel tools to attenuate (by deletion of virulence factor) and to generate multivalent (by insertion of heterologous genes) vaccine vectors to protect chickens against pathogens of poultry and public health importance

Melanoma and COVID-19: A narrative review focused on treatment

Melanoma is the most severe form of skin cancer and its incidence has increased over the past few decades. COVID-19 pandemic affected the diagnosis and management of many diseases including melanoma. In this study, we aimed to provide a review focused on the diagnosis and management of melanoma in the era of COVID-19. A comprehensive search was conducted on PubMed, Web of Science, and Google Scholar databases using the keywords “melanoma,” “coronavirus,” “COVID 19,” and “SARS-CoV-2.” The relevant guidelines published by the European Society for Medical Oncology and the National Comprehensive Cancer Network were also included. The current guidelines recommend that surgical interventions for new diagnosis of invasive primary melanoma, patients with postoperative complications, wide resection and sentinel lymph node biopsy for newly diagnosed T3-T4 melanoma, and planned surgical procedures for patients in neo-adjuvant trials should be prioritized. Surgical treatment of T3/T4 melanomas should be prioritized over T1/T2 melanomas except for any melanoma in which large clinical residual lesion is visible. Adjuvant therapies can be postponed for up to 12 weeks depending on the local center circumstances. PD-1 inhibitor monotherapy is recommended for patients starting immunologic therapy. Combination immunotherapy is still considered suitable for patients with higher-risk disease. Encorafenib and binimetinib should be prioritized for patients requiring BRAF-targeted therapy due to the lower chance of symptoms mimicking COVID-19 infection. © 2020 Wiley Periodicals LLC