Mice fetus liver tissue spectroscopic alterations following concomitant administration of Metronidazole & Miconazole

Introduction: Metronidazole (MET) and Miconazole (MIC) are antiprotozoal and imidazole antifungal agents, respectively. The aim of this study was to assess the usefulness of Fourier Transform Infrared micro-spectroscopy (FTIR-MSP) for discriminating and detecting spectral changes of mouse fetus liver tissue after mother’s exposure to concomitant use of MET and MIC. Methods and Results: The control group received only normal diet and the exposure groups received MET, MIC, and concomitant MET and MIC. The pregnant mice were anesthetized and their fetuses were surgically removed on gestation day 15. Fixative slides without any staining were put on KBr disc with a 1mm thick for IR micro spectroscopy. All information obtained from the spectra were analyzed using routine FTIR software. Based on our results, MET and MIC, alone and in combination together, have created some changes in the mice fetus liver biomolecules. Conclusions: FTIR biospectroscopy application in teratology is very challenging, but it has been accepted as a reliable technique; however, it is necessary to do more investigations

Processing on recognition of FTIR-MSP alteration of Heart tissue during mice fetal life

Introduction: Understanding of fetus development is one of the most complicated with a great impact on Teratology. FTIR-MSP is among the most useful spectroscopy technique for biological and cellular application. In this project, various statistical calculations were used for recognition and discrimination of heart tissue spectra during 9.5-17.5 days of mice fetal life. Method and Results: The mice fetuses were dissected on day 9.5-17.5 of gestation and then fixed by fixative solution. Tissue sections (10 µm) were used for FTIR-MSP measurement in the wavenumber region of 4000-400 cm-1. Spectra were preceded by baseline correction, smoothing, deconvolution and 2nd derivatisation. PCA, ANN and SVM have been used to find the most relevant modifications in during fetus development. PCA with adjusting data mass and seven selected major PCs have been used to find the most relevant modifications in different steps of mice fetus heart tissue development and also BP-FF ANN and SVM classifications could diagnose different steps of development up to 96.3% and 92.59% respectively. Conclusions: PCA, ANN and SVM methods could classify and discriminate the FTIR spectroscopic data and can be as a new potential tool for the teratogenic investigations

ArticleFTIR Determination of MiconazoleEffects on Mice Fetus Brain Tissue: FTIR determination of Miconazole effects on Mice fetus brain tissue

Miconazole is an imidazole antifungal agent, commonly applied topically to the skin or mucous membranes. The aim of this study was to examine the alternative method for gaining mechanism or the bimolecular changes caused by the possible teratogenic effects of Miconazole on mice fetus brain tissueusing FTIR-Microspectroscopy.The mice were injected with Miconazole(60 mg/Kg) on gestation day 9. Fetuses were dissected on day 15 of gestation and morphological and histological studies on thefetus were carried out. Sections (10 μm) of controland Miconazoletreated fetus brain tissue were used for FTIR measurement in the mid-infraredregion. The results were shown by spectra2ndderivativeand also subtracting from control spectra.A lower intensity in the lipid (2800–3000 cm-1) and amid I (1600–1800 cm-1) regions of Miconazole treated mice fetus brain tissue was observed compared to the control mice fetus brain tissue. No major spectral shifting was observed at amide I band, amide II band and nucleic acid regions.As a conclusion, FTIR-Microspectroscopycan be a useful tool forteratogenic measurement with a unique ability to identify the modified bimolecular structuresin mice fetus tissues