Effects of acute L-carnitine supplementation on nitric oxide production and oxidative stress after exhaustive exercise in young soccer players

Erikoğlu Örer, G. (Aksaray, Yazar)Aim. The aim of this study was to investigate the effect of acute L-carnitine supplementation at two different doses on nitric oxide (NO) production and oxidative stress after exhaustive exercise. Methods. The subjects were 26 healthy males aged 17-19 years. Subjects were randomly divided into two groups of 13 each and received 3 or 4 g L-carnitine via a glass of fruit juice. After 1 h, the treadmill test was started at a speed of 8 km/h, afterwards increasing the speed 1 km/h every 3 min with a 1 min rest before every speed increase until exhaustion. Venous blood samples were collected again within 5 min following the exercise test. One week later, the test was repeated on the same athletes with placebo fluids. All subjects received a placebo via a glass of fruit juice. 1 h after the same exercise protocol mentioned above was performed and blood samples were drawn immediately. These plasma samples were used for measurement of nitrate-nitrite (NOx), which are known to be the stable end products of NO, TBARs as a lipid peroxidation indicator and an antioxidant glutathione (GSH) levels. Results and conclusion. The results of this study have shown that L-carnitine applied at a 3 g dose provides strong antioxidant action by increasing the GSH and NOx level and decreasing the TBARs level

The effects of thyme tea supplement on free radicals formation and antioxidant system of elite wrestlers

The intensive acute exercises results in too much oxygen consumption and hence produce excessive free radicals and damage of tissues. This study was conducted to investigate the effects of thyme tea on free radical formation and antioxidant system. Eighteen elite volunteer wrestlers joined the study. The wrestlers were randomly divided into two groups to from experiment and control groups. The subjects wrested five times during the study period according to F.I.L.A rules. The measurement are performed before and after the thyme tea loading. Study groups drunk thyme tea three times a day in 35 day period before loading after first measurement. The subjects participated in the study pre-contest (Comp. Before), immediately after the competition (Comp. After), 24 hours after the competition (Comp. after 24 h), 48 hours after the competition (Comp. After 48 h), blood samples were collected from forearm vein by 5cc total of 2 tubes. Tubes, centrifuge immediately after serums and transferred to tubes Eppendorf Total of Antioxidant o Capacity (TAC), Malondialdehyde (MDA) and total sulfhydryl group (RSH) stored at the 75 C until determination of analysis. The results showed that no difference was noted between the values of the groups before the thyme loading process, a meaningful increase (p0.05). Whereas significant differences were found in the levels of MDA and TAC, after thyme loading, for the experimental group when compared to the control group (p<0.05), no meaningful difference was observed for the level of RSH. it is determined in the study that wrestling competition causes significant oxidant stress in wrestlers and increase total antioxidant capacity. However, it is detected that it makes nonenzymatic antioxidant capacity fall. © Asian Network for Scientific Information, 2013