Additional file 1: of SOFIA®RSV: prospective laboratory evaluation and implementation of a rapid diagnostic test in a pediatric emergency ward

Study 2- SOFIA®RSV satisfaction survey. This survey was sent to the 13 pediatric consultants that were working in the ED during the study 2 period, 5 months after the end of the study. It includes 4 questions and one space for comments. (DOCX 11 kb

Global comparison of PPV, Sensitivity, NPV, Specificity and Youden's index values between kits for all tests.

<p>Criteria for the definition of true positives, false positives, true negatives and false negatives are provided in the 'Materials and Methods section', 'Performances of the kits' sub-section. PneumoVir: CLART PneumoVir; xTagRVP-F: xTAG Respiratory Viral Panel Fast; FTD-RP33: Fast Track Diagnostics Respiratory Pathogen 33; RespiFinder: RespiFinder SMART 22</p><p>Global comparison of PPV, Sensitivity, NPV, Specificity and Youden's index values between kits for all tests.</p

Respiratory pathogens detected by each Kit.

<p>Inf A, B, C: Influenza A, B, C; H1N1: Influenza A H1N1; H1N1pdm09: Influenza A H1N1pdm 2009; H3N2: Influenza A H3N2; PIV 1, 2, 3, 4, 4a, 4b: Parainfluenzavirus 1, 2, 3, 4, 4a, 4b; hRSV: human Respiratory Syncytial Virus; hRSV A, B: human Respiratory Syncytial Virus A, B; EV: Enterovirus; hRV: human Rhinovirus; AdV: Adenovirus; hBoV: human Bocavirus; hCoV 229E, OC43, NL63, HKU1: human Coronavirus type 229, OC43, NL63, HKU1; hMPV: human Metapneumovirus; hMPV A, B: human Metapneumovirus A, B; CMV: Cytomegalovirus; EVs: Echovirus; hPeV: human Parechovirus; Pneumo jiro: <i>Pneumocystis jirovecii;</i> Myco pneumo: <i>Mycoplasma pneumoniae;</i> Chlam pneumo: <i>Chlamydia pneumoniae;</i> Strepto pneumo: <i>Streptococcus pneumoniae;</i> Haemo inf sp: <i>Haemophilus influenzae</i> species; Haemo inf B: <i>Haemophilus influenzae</i> type B; Staph aur: <i>Staphylococcus aureus;</i> Morax catarr: <i>Moraxella catarrhalis;</i> Bord pert: <i>Bordetella pertussis;</i> Klebs pneumo: <i>Klebsiella pneumoniae;</i> Legio sp: <i>Legionella</i> species; Salmo sp: <i>Salmonella</i> species; PneumoVir: CLART PneumoVir; FTD-RP33: Fast Track Diagnostics Respiratory Pathogen 33; xTagRVP-F: xTAG Respiratory Viral Panel Fast; RespiFinder: RespiFinder SMART 22</p><p>Respiratory pathogens detected by each Kit.</p

Viral etiologies identified and bacterial results.

<p>Inf A, B: Influenza A, B; H1N1: Influenza A H1N1; H1N1pdm09: Influenza A H1N1pdm 2009; PIV 1, 2: Parainfluenzavirus 1, 2; hRSV A, B: human Respiratory Syncytial Virus A, B; EV: Enterovirus; hRV: human Rhinovirus; AdV: Adenovirus; hBoV: human Bocavirus; hCoV 229, OC43, NL63, HKU1: human Coronavirus type 229, OC43, NL63, HKU1; hMPV A, B: human Metapneumovirus A, B.</p><p>(*) EV/ hRV detected no determined for Enterovirus or human Rhinovirus</p><p>(**) Bacteria etiologies identified are shown in supplementary information (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130378#pone.0130378.s002" target="_blank">S1 Table</a>)</p><p>Viral etiologies identified and bacterial results.</p

Decision tree (algorithm used for Core tests).

<p>(Inf A: Influenza A, Inf B: Influenza B, hRSV: human Respiratory Syncytial Virus, hMPV: human Metapneumovirus, EV + hRV:; Enterovirus + human Rhinovirus, AdV: Adenovirus and hBoV: human Bocavirus).</p

Performances of the four kits for individual Core tests (Inf A, Inf B, hRSV, hMPV, EV + hRV, AdV and hBoV), parainfluenza viruses, Influenza A H1N1pdm 2009 and coronaviruses.

<p>Criteria for the definition of true positives, false positives, true negatives and false negatives are provided in the 'Materials and Methods' section, 'Performances of the kits' sub-section.</p><p>^a Kit having only probe for the detection of Coronavirus E229, and not for HKU1, OC43 and NL63.</p><p>^b Kit having probe for the detection all Influenza A viruses and not for specific detection of H1N1 2009 pdm.</p><p>Performances of the four kits for individual Core tests (Inf A, Inf B, hRSV, hMPV, EV + hRV, AdV and hBoV), parainfluenza viruses, Influenza A H1N1pdm 2009 and coronaviruses.</p

Estimate of detection limit of the different kits.

<p>Inf A H1N1: Influenza A H1N1 (A/Marseille/9410380/2009), Inf A H3N2: Influenza A H3N2 (A/Marseille/4781598/2009), Inf B: Influenza B (B/Marseille/4461097/2008), Inf C: Influenza C (C/Leningrad:232/83), hRSV A and B: human Respiratory Syncytial virus type A (Long) and B (Gb2), hRV: Rhinovirus (MAR2007 3813047), EV Cox B3: Enterovirus (Human Coxsackievirus B3 clinical strain B32679), hPeV: human Parechovirus type 3 (UNK), AdV B: Adenovirus type B (MAR2007 7416184 clinical strain), and hMPV A: human Metapneumovirus type A.</p

List of pathogens that were considered to be present in the panel of the 88 specimens according the combination of duplex PCR tests (Argene/bioMérieux) used as gold standard.

a<p>type 1 (n = 2), type 3 (n = 3) and type 4 (n = 5).</p>b<p>type NL63 (n = 6), type OC43 (n = 8), type 229E (n = 1), type HKU1 (n = 6) and untyped (n = 4).</p

Presentation of the 7 kits used in the study for detecting viruses (see text for correspondence between letters and commercial denominations).

<p>Yes: group of pathogens detected; w diff: with differentiation between types. No: group of pathogens not detected.</p

Practicability of each kit using the NUCLISENS easyMAG as extraction instrument (see text for correspondence between letters and commercial denominations).

<p>Practicability of each kit using the NUCLISENS easyMAG as extraction instrument (see text for correspondence between letters and commercial denominations).</p