Exploration of constitutive and induced markers of disease severity caused by Botrytis cinerea in the primary metabolome of tomato

International audienceConstitutive markers of plant defense are required to provide solutions for plant protection by the control of its environment. While dozens of plant secondary metabolites have been identified as putative markers of defense against necrotrophic fungi, few where robustly related to disease intensity. In this work, we explored the potential of primary metabolites as predictors of disease severity. Plant soluble sugars, as main components of primary metabolism, are thought to be implicated in defense against pathogenic fungi. However, the function of sucrose and hexoses remains unclear. A different role for glucose and fructose in tomato defense against Botrytis cinerea was hypothesized. We examined tissue contents in primary metabolites at the time of inoculation with B. cinerea for tomato plants subjected to a range of abiotic environments created by various nitrogen and water supplies. Sugar contents and defense hormonal markers were then quantified during the infection process. In a first step, we assessed multiple combinations of primary metabolites as possible constitutive markers of disease intensity. In a second step, we checked for the kinetics of the selected markers during the infection process. Ratios of soluble sugars, rather than those of organic or amino-acids where identified during the first step as best correlators of disease intensity. Soluble sugar contents of tissues surrounding infection sites evolved differently after inoculation. The fructose content never decreased after inoculation with B. cinerea, while that of glucose and sucrose showed either positive or negative variation, depending on the abiotic environment. Increase in relative fructose content (defined as the proportion of fructose in the soluble sugar pool), was observed in absence of glucose accumulation, and was associated with lower disease intensity. A lower expression of the salicylic acid marker PR1a, and a lower repression of a jasmonate marker COI1 was associated with higher relative fructose contents. We concluded that fructose relative content was both a constitutive and induced marker of symptoms in a range of environments. Small variations of fructose content among the sugar pool are unlikely to affect pathogen growth by itself. Our data strongly suggest that the adjustment of relative fructose content is required for enhanced defense against B. cinerea

Reduced susceptibility of tomato stem to the necrotrophic fungus Botrytis cinerea is associated with a specific adjustment of fructose content in the host sugar pool

Background and aims Plant soluble sugars, as main components of primary metabolism, are thought to be implicated in defence against pathogenic fungi. However, the function of sucrose and hexoses remains unclear. This study aimed to identify robust patterns in the dynamics of soluble sugars in sink tissues of tomato plants during the course of infection by the necrotrophic fungus Botrytis cinerea. Distinct roles for glucose and fructose in defence against B. cinerea were hypothesized.Methods We examined sugar contents and defence hormonal markers in tomato stem tissues before and after infection by B. cinerea, in a range of abiotic environments created by various nitrogen and water supplies.Key Results Limited nitrogen or water supplies increased tomato stem susceptibility to B. cinerea. Glucose and fructose contents of tissues surrounding infection sites evolved differently after inoculation. The fructose content never decreased after inoculation with B. cinerea, while that of glucose showed either positive or negative variation, depending on the abiotic environment. An increase in the relative fructose content (defined as the proportion of fructose in the soluble sugar pool) was observed in the absence of glucose accumulation and was associated with lower susceptibility. A lower expression of the salicylic acid marker PR1a, and a lower repression of a jasmonate marker COI1 were associated with reduced susceptibility. Accordingly, COI1 expression was positively correlated with the relative fructose contents 7 d after infection.Conclusions Small variations of fructose content among the sugar pool are unlikely to affect intrinsic pathogen growth. Our results highlight distinct use of host glucose and fructose after infection by B. cinerea and suggest strongly that adjustment of the relative fructose content is required for enhanced plant defence