Soil fertility gradients and production constraints for coffee and banana on volcanic mountain slopes in the east African rift: a case study of Mt. Elgon

Volcanic mountains in the East African Rift (e.g. Mt. Kenya, Mt. Kilimanjaro, Mt. Elgon) are some of the most productive agricultural regions, often dominated by coffee and banana cultivation. Consequently, these regions suffer from a high and increasing population density with a declining soil fertility status imposing pressure on the available land, which in turn results in encroaching into the national forests. This study documents the soil fertility constraints along the slopes of Mt. Elgon and explores its corresponding gradients in plant nutritional status. This research links the topography of Mt. Elgon to the prevailing soil types and their current fertility status. It reveals important relations and gradients between soil fertility parameters and its corresponding environment along the slope. Soil pH, soil available P and exchangeable K, Ca and Mg are significantly decreasing with elevation. Thereby, gradients and constraints in macro- and micro-nutrient uptake by coffee and banana are revealed along the toposequence and different altitude-specific nutrient limitations are determined for both crops. K, Mn and Si uptake in both crops is decreasing with elevation along the slope, while the Mo and Ni uptake in both crops is increasing. With increasing elevation, B uptake is only decreasing in coffee and P uptake is only decreasing in banana. In addition, the antagonistic interaction between K and Mg limits the Mg uptake of both crops in the lower areas, while in the high region the Mg uptake is simply limited by low soil availability. It follows that a general fertilizer recommendation cannot be made in these regions and that the soil fertility problems along these slopes should be specifically addressed and appropriately managed according to the local requirements

Cost-minimisation analysis of a treat-and-extend regimen with anti-VEGFs in patients with neovascular age-related macular degeneration

PURPOSE: Although intraocular anti-vascular endothelial growth factors (anti-VEGFs) are effective as treatment of neovascular age-related macular degeneration (nAMD), the (economic) burden on the healthcare system is considerable. A treat-and-extend (T&E) regimen is associated with a lower number of injections without compromising the effectiveness and can therefore help optimise nAMD treatment. This study investigates the per-patient costs associated with nAMD treatment, when using aflibercept, bevacizumab, or ranibizumab with a T&E regimen. METHODS: In this cost-minimisation model, the per-patient costs in the Netherlands were modelled using a healthcare payers’ perspective over a 3-year time horizon with the assumption that efficacy of treatments is similar. Additionally, the break-even price of the different anti-VEGFs was calculated relative to the cheapest option and injection frequency. RESULTS: The injection frequency varied from 14.2 for aflibercept to 27.4 for bevacizumab in 3 years. Nonetheless, bevacizumab remains the cheapest treatment option (€14,215), followed by aflibercept (€18,202) and ranibizumab (€31,048). The medication covers the majority of the per-patient costs for aflibercept and ranibizumab, while administration covers the majority of the per-patient costs for bevacizumab. The break-even prices of aflibercept and ranibizumab are respectively €507 and €60.58 per injection. Brolucizumab was included in the scenario analysis and was more expensive than aflibercept (€20,446). Brolucizumab should reduce to 13.8 injections over 3 years to be as costly as aflibercept. CONCLUSION: Bevacizumab is the cheapest anti-VEGF treatment. The list prices of all anti-VEGFs should reduce to be as costly as bevacizumab. Aflibercept is the second-choice treatment and so far brolucizumab is not

Short mucin 6 alleles are associated with H pylori infection

Contains fulltext : 49314.pdf (publisher's version ) (Open Access)AIM: To investigate the relationship between mucin 6 (MUC6) VNTR length and H pylori infection. METHODS: Blood samples were collected from patients visiting the Can Tho General Hospital for upper gastrointestinal endoscopy. DNA was isolated from whole blood, the repeated section was cut out using a restriction enzyme (Pvu II) and the length of the allele fragments was determined by Southern blotting. H pylori infection was diagnosed by (14)C urea breath test. For analysis, MUC6 allele fragment length was dichotomized as being either long (> 13.5 kbp) or short (< or = 13.5 kbp) and patients were classified according to genotype [long-long (LL), long-short (LS), short-short (SS)]. RESULTS: 160 patients were studied (mean age 43 years, 36% were males, 58% H pylori positive). MUC6 Pvu II-restricted allele fragment lengths ranged from 7 to 19 kbp. Of the patients with the LL, LS, SS MUC6 genotype, 43% (24/56), 57% (25/58) and 76% (11/46) were infected with H pylori, respectively (P = 0.003). CONCLUSION: Short MUC6 alleles are associated with H pylori infection

Participation without Negotiating: Influence of Stakeholder Power Imbalances and Engagement Models on Agricultural Policy Development in Uganda

Although the political context in Uganda exhibits democratic deficit and patronage, research and development actors have given little attention to the possible negative impact these may have on agricultural policymaking and implementation processes. This article examines the influence of power in perpetuating prevailing narratives around public participation in agricultural policymaking processes. The analysis is based on qualitative data collected between September 2014 and May 2015 using 86 in‐depth interviews, 18 focus group discussions, and recorded observations in stakeholder consultations. Results indicate that while the political setting provides space for uncensored debates, the policymaking process remains under close control of political leaders, technical personnel, and high‐level officers in the government. Policy negotiation remains limited to actors who are knowledgeable about the technical issues and those who have the financial resources and political power to influence decisions, such as international donors. There is limited space for negotiation of competing claims and interests in the processes by public and private actors actively engaged in agricultural development, production, processing, and trade. Thus, efforts to achieve good governance in policy processes fall short due to lack of approaches that promote co‐design and co‐ownership of the policies

National AIDS Control Programme:AIDS Surveillance,Report No.4

This report covers the status of the HIV/AIDS epidemic in Tanzania main land by December 1990, and contains updated figures since the third report of August 1990. Moreover, data were analyzed more in depth, to reveal any trends. Maps on AIDS cases and HIV prevalence have been added as well. Figures from various sources all indicate that the HIV/AIDS epidemic continues to increase at alarming rates throughout Tanzania. Two groups are of particular importance : Antenatal clinic attenders and adolescents: Among pregnant women, attending Antenatal clinics in Mbeya, Mwanza and Bukoba region, the percentage HIV-positive women has increased from 10% to 16% (Mbeya) and from 8% to 14% (Mwanza) in little over a year. In Bukoba the percentage of infected women rose from 20.8 to 23.3. The effect on the infant mortality rate will be considerable : as 30% of children born to these women will die from AIDS within the first few years of their life, up to 5% of newborns (50 per 1,000) in Mwanza and Mbeya towns are expected to die from AIDS. Children escaping infection with HIV(up to 11%) are unlikely to have a mother (or any parent) still alive by the end of the century. Although a similar situation might not prevail throughout the country, data from bloodtransfusion services throughout the country suggest that the problem is virtually nationwide. As previously reported, a second group of great concern are adolesccents (15-19 year old) : data from blood donors show an alarming increase among the 15-19 and 20-24 year agegroups. Among 15-19 year old, the percentage seropositives was 0.0% in 1987,increased rapidly thereafter, and has reached fivefold from 1.6% to 8.2% between 1987 and 1990. Further analysis revealed that the situation among adolescents was more serious for girls than boys. In the light of these facts, there remains an urgent need to review programme strategies, in order to come up with interventions which will bring trends to a halt. Projections of AIDS Cases reported during the 1990’s are presented as well. Even if transmission of HIV would cease as from now, from the estimated number of approx. 800,000 HIV infected persons 450,000 will develop AIDS during the remainder of this decade. If transmission continues up to 1995 at a rate of 1% new HIV infections per year, 750,000 will have developed AIDS by the year 2,000

Induction of antigen-specific antibody response in human pheripheral blood lymphocytes in vitro by a dog kidney cell vaccine against rabies virus (DKCV).

In the present report an in vitro method for obtaining a secondary human antibody response to a dog kidney cell vaccine against rabies virus (DKCV) is described. Cultures of peripheral blood mononuclear cells from normal rabies-immune and nonimmune donors were stimulated in vitro by DKCV. The production of virus-specific antibody in supernatant fluids was monitored by ELISA. Antibody was produced by lymphocytes from rabies-immune individuals, whereas those of nonimmune subjects consistently failed to produce anti-rabies antibodies after in vitro stimulation with DKCV. The generation of the anti-rabies virus antibody response of lymphocytes stimulated with DKCV was shown to be an antigen-dependent, as well as an antigen-specific process. Optimal antigen-specific response

Characterization of fastidious adenovirus types 40 and 41 by DNA restriction enzyme analysis and by neutralizing monoclonal antibodies.

The DNA of 48 strains of adenovirus type 40 (Ad40) and of 128 strains of adenovirus type 41 (Ad41), isolated between 1971 and 1986 from various countries, was characterized by restriction enzyme analysis using nine and ten restriction endonucleases respectively. Five new DNA variants of Ad40 and 18 new DNA variants of Ad41 were detected. Most of the restriction sites which differed among the various DNA variants appeared to be distributed at random over the entire length of the viral genomes of the two serotypes. The number of restriction sites by which two DNA variants differed from each other was used as a measure of their relatedness. Several clusters of closely related DNA variants were observed for each of the two serotypes. The 35 DNA variants of Ad40 and Ad41 were used to test monoclonal antibody preparations for their range of reactivity in a neutralization assay. One monoclonal antibody (5-8), raised against Ad40 strain Dugan, showed type-specific neutralization of all 11 Ad40 DNA variants tested. Six monoclonal antibodies, raised against Ad41 strain Tak, neutralized different proportions of the variants of Ad41. Two of these preparations (1-21 and 3-19) neutralized all 24 Ad41 DNA variants, while a third (1-23) reacted with only 12 Ad41 variants. Three other monoclonal antibody preparations (3-10, 3-18, 7-14) reacted specifically with only 6 of these 12 variants. The patterns of reactivity with the monoclonal antibody preparations correlated with the presence or absence of a HindIII restriction site at 56 map units and of an EcoRI restriction site at 52 map units on the Ad41 DNA. This region of the adenovirus DNA codes for the hexon protein, which is known to contain the type-specific neutralizing antigenic determinants

Characterization of fastidious adenovirus types 40 and 41 by DNA restriction enzyme analysis and by neutralizing monoclonal antibodies.

The DNA of 48 strains of adenovirus type 40 (Ad40) and of 128 strains of adenovirus type 41 (Ad41), isolated between 1971 and 1986 from various countries, was characterized by restriction enzyme analysis using nine and ten restriction endonucleases respectively. Five new DNA variants of Ad40 and 18 new DNA variants of Ad41 were detected. Most of the restriction sites which differed among the various DNA variants appeared to be distributed at random over the entire length of the viral genomes of the two serotypes. The number of restriction sites by which two DNA variants differed from each other was used as a measure of their relatedness. Several clusters of closely related DNA variants were observed for each of the two serotypes. The 35 DNA variants of Ad40 and Ad41 were used to test monoclonal antibody preparations for their range of reactivity in a neutralization assay. One monoclonal antibody (5-8), raised against Ad40 strain Dugan, showed type-specific neutralization of all 11 Ad40 DNA variants tested. Six monoclonal antibodies, raised against Ad41 strain Tak, neutralized different proportions of the variants of Ad41. Two of these preparations (1-21 and 3-19) neutralized all 24 Ad41 DNA variants, while a third (1-23) reacted with only 12 Ad41 variants. Three other monoclonal antibody preparations (3-10, 3-18, 7-14) reacted specifically with only 6 of these 12 variants. The patterns of reactivity with the monoclonal antibody preparations correlated with the presence or absence of a HindIII restriction site at 56 map units and of an EcoRI restriction site at 52 map units on the Ad41 DNA. This region of the adenovirus DNA codes for the hexon protein, which is known to contain the type-specific neutralizing antigenic determinants