Membranous glomerulonephritis in the mouse

Membranous glomerulonephritis in the mouse. Glomerulonephritis was induced in C57.B110 mice by a single injection of rabbit IgG against homologous, pronase-digested, renal tubular antigens. The heterologous phase was characterized by a transient increase of glomerular permeability with fixation of rabbit IgG to the capillary walls, in a linear or fine-granular pattern, and to the brush borders of the proximal tubuli. The autologous phase was marked by the immune response to the injected protein, during which subepithelial immune deposits, consisting of mouse IgG1, rabbit IgG, and mouse C3 developed. Small amounts were still present at 1 year after the injection of antiserum. The antibody response of the mice correlated with the development and resolution of the deposits. None of the mice developed a nephrotic syndrome. Control mice treated with normal rabbit IgG did not show immune deposits in their kidneys at any stage despite a comparable antibody response to rabbit IgG. Immunoelectronmicroscopy showed that the rabbit antibodies fixed directly to an antigen in the cell membrane of the glomerular visceral epithelium. It seems, therefore, likely that in situ formation of subepithelial immune complexes occurred in the autologous phase by fixation of mouse immunoglobulins to rabbit IgG already present in the glomerular wall.Glomérulonéphrite extra-membraneuse chez la souris. Une glomérulonéphrite a été induite chez des souris C57.B110 par une injection unique d'IgG de lapin contre des antigènes tubulaires rénaux homologues, digérés par de la pronase. La phase hétérologue était caractérisée par une augmentation transitoire de la perméabilité glomérulaire avec fixation d'IgG de lapin aux parois capillaires, d'une façon linéaire ou finement granuleuse, et aux bordures en brosse des tubules proximaux. La phase autologue était marquée par la réponse immune à la protéine injectée, pendant laquelle des dépôts immuns sous-épithéliaux, consistant en de l'IgG1 de souris, de l'IgG de lapin et du C3 de souris, se sont développés. Il en restait encore de faibles quantités 1 an après l'injection de l'antisérum. La réponse anticorps des souris était corrélée avec le développement et la disparition des dépôts. Aucune des souris n'a développé de syndrome néphrotique. Les souris contrôles traitées avec de l'IgG de lapin normal n'ont pas eu de dépôts immuns dans le rein à aucun stade, malgré une réponse anticorps aux IgG de lapin comparable. La microscopie immuno-électronique a montré que les anticorps de lapin se fixaient directement à un antigène situé sur la membrane des cellules de l'épithélium viscéral glomérulaire. Il semble donc probable que la formation in situ de complexes immuns sous-épithéliaux est survenue à la phase autologue par fixation d'immunoglobulines de souris à de l'IgG de lapin déjà présente dans la paroi glomérulaire

Oceanographic observations at the shelf break of the Amundsen Sea, Antarctica

The part of the West Antarctic Ice Sheet that drains into the Amundsen Sea is currently thinning at such a rate that it contributes nearly 10% of the observed rise in global mean sea level. Acceleration of the outlet glaciers appears to be caused by thinning at their downstream ends, where the ice goes afloat, indicating that the changes are probably being forced from the ocean. Observations made since the mid-1990s on the Amundsen Sea continental shelf have revealed that the deep troughs, carved by previous glacial advances, are flooded by almost unmodified Circumpolar Deep Water (CDW) with temperatures around 3-4°C above the freezing point, and that this water mass drives rapid melting of the floating ice. Here we report observations of water properties and currents made in the region where one of those troughs reaches the continental shelf edge. We estimate the absolute circulation within the trough from a combination of detided Acoustic Doppler Current Profiler data and geostrophic shear derived from Conductivity-Temperature-Depth sections. The shelf edge region is characterised by a landward deepening of the pycnocline separating CDW from the overlying colder and fresher surface waters. This feature, the so-called Antarctic Slope Front (ASF), is almost circumpolar in extent, and is typically a full-depth feature, the pycnocline intersecting the seabed over the upper continental slope. However, the ASF is weaker in the Amundsen Sea, where it is rarely a full-depth feature. Geostrophic shear associated with the ASF leads to a weakening of the associated westward current with depth, and an eastward undercurrent of varying strength has been reported at other locations. At the time of our Amundsen Sea observations the westward surface flow was weak, giving rise to a strong eastward undercurrent flowing along the continental shelf edge and upper slope. At the upstream (western) side of the trough the undercurrent turns south, driving a net on-shelf flow of CDW in the western part of the trough, and leaving a weakened shelf edge flow that re-establishes itself on the downstream (eastern) side of the trough. An analogous feature was captured, albeit crudely, in an earlier coarse-resolution model of the circulation on the Amundsen Sea shelf, and variability in its strength, associated with variability in the surface wind stress, was the main cause of variations in the heat content of the waters on the inner continental shelf. Our observations thus lend support to the earlier hypothesis that changes in atmospheric forcing over the continental shelf edge could be the ultimate driver of changes in the West Antarctic Ice Sheet outlet glaciers that drain into the Amundsen Sea

Beam Dynamics and Tolerance Studies of the THz-driven Electron Linac for the AXSIS Experiment

A dielectric-loaded linac powered by THz-pulses is one of the key parts of the "Attosecond X-ray Science: Imaging and Spectroscopy" (AXSIS) project at DESY, Hamburg. As in conventional accelerators, the AXSIS linac is designed to have phase velocity equal to the speed of light which, in this case, is realized by tuning the thickness of the dielectric layer and the radius of the vacuum channel. Therefore, structure fabrication errors will lead to a change in the beam dynamics and beam quality. Additionally, errors in the bunch injection will also affect the acceleration process and can cause beam loss on the linac wall. This paper numerically investigates the process of electron beam acceleration in the AXSIS linac, taking into account the aforementioned errors. Particle tracking simulations were done using the code ECHO, which uses a low-dispersive algorithm for the field calculation and was specially adapted for the dielectric-loaded accelerating structures.Comment: EAAC'17 conference proceeding

Electron trapping and acceleration by the plasma wakefield of a self-modulating proton beam

It is shown that co-linear injection of electrons or positrons into the wakefield of the self-modulating particle beam is possible and ensures high energy gain. The witness beam must co-propagate with the tail part of the driver, since the plasma wave phase velocity there can exceed the light velocity, which is necessary for efficient acceleration. If the witness beam is many wakefield periods long, then the trapped charge is limited by beam loading effects. The initial trapping is better for positrons, but at the acceleration stage a considerable fraction of positrons is lost from the wave. For efficient trapping of electrons, the plasma boundary must be sharp, with the density transition region shorter than several centimeters. Positrons are not susceptible to the initial plasma density gradient.Comment: 9 pages, 9 figures, 1 table, 44 reference