MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease

Introduction: Diabetic kidney disease (DKD) is a common microvascular complication that affects 40% of patients with diabetes mellitus (DM). Emerging evidence suggests a role for several microRNAs (miRNAs) in the development of DKD. In this context, miR- 15a-5p and miR-30e-5p have been shown to regulate the expression of the uncoupling protein 2 (UCP2), a mitochondrial protein that decreases reactive oxygen species (ROS) formation by the mitochondria. Since ROS overproduction is a key contributor to the pathogenesis of DKD, dysregulation of these two miRNAs could be involved in DKD pathogenesis. Thus, the aim of this study was to compare the expressions of miR- 15a-5p and miR-30e-5p in type 1 DM (T1DM) patients with DKD (cases) and without this complication (controls), and to perform bioinformatics analyses to investigate their putative targets and biological pathways under their regulation. Methods: MiR-15a-5p and miR-30e-5p expressions were analyzed in plasma and urine of 17 T1DM controls and 23 DKD cases (12 with moderate DKD and 11 with severe DKD) using qPCR. Bioinformatics analyses were performed in Cytoscape software. Results: MiR-30e-5p expression was downregulated in plasma of patients with moderate and severe DKD compared to T1DM controls. Moreover, this miRNA was also downregulated in urine of patients with severe DKD compared to the other groups. No difference was found in miR-15a-5p expression between groups. Bioinformatics analyses indicated that miR-30e-5p and miR-15a-5p regulate various genes that participate in pathways related to angiogenesis, apoptosis, cell differentiation, oxidative stress, and hypoxia. Conclusion: MiR-30e-5p seems to be downregulated in plasma and urine of patients with DKD

TIREOIDITE AGUDA

A tireoidite aguda (TA) é uma entidade rara entre as enfermidades da tireoide, porém sua incidência tem se elevado devido ao aumento do número de pacientes imunodeprimidos, bem como devido a anormalidades congênitas como fendas branquiais ou fístulas do seio piriforme, localizado entre a glândula tireoide e a hipofaringe. As infecções tireoidianas são patologias graves que merecem especial atenção em função de oferecer grande risco de complicação. Quando baseada em uma avaliação clínica rigorosa e capacitada, além de amparada por métodos diagnósticos por imagem e laboratoriais, seu desfecho é positivo. O objetivo deste trabalho é realizar uma revisão acerca da etiopatogenia, dos diagnósticos clínico e laboratorial e do tratamento da Tireoidite Supurativa Aguda.Palavras-chave: Tireoidite Supurativa Aguda. Abscesso Tireoidiano. Fístula do Seio Piriforme