123 research outputs found
Effect of macromolecular crowding on the rate of diffusion-limited enzymatic reaction
The cytoplasm of a living cell is crowded with several macromolecules of
different shapes and sizes. Molecular diffusion in such a medium becomes
anomalous due to the presence of macromolecules and diffusivity is expected to
decrease with increase in macromolecular crowding. Moreover, many cellular
processes are dependent on molecular diffusion in the cell cytosol. The
enzymatic reaction rate has been shown to be affected by the presence of such
macromolecules. A simple numerical model is proposed here based on percolation
and diffusion in disordered systems to study the effect of macromolecular
crowding on the enzymatic reaction rates. The model explains qualitatively some
of the experimental observations.Comment: 6 pages, 4 figure
Parallel Evolution of Auditory Genes for Echolocation in Bats and Toothed Whales
The ability of bats and toothed whales to echolocate is a remarkable case of convergent evolution. Previous genetic studies have documented parallel evolution of nucleotide sequences in Prestin and KCNQ4, both of which are associated with voltage motility during the cochlear amplification of signals. Echolocation involves complex mechanisms. The most important factors include cochlear amplification, nerve transmission, and signal re-coding. Herein, we screen three genes that play different roles in this auditory system. Cadherin 23 (Cdh23) and its ligand, protocadherin 15 (Pcdh15), are essential for bundling motility in the sensory hair. Otoferlin (Otof) responds to nerve signal transmission in the auditory inner hair cell. Signals of parallel evolution occur in all three genes in the three groups of echolocators—two groups of bats (Yangochiroptera and Rhinolophoidea) plus the dolphin. Significant signals of positive selection also occur in Cdh23 in the Rhinolophoidea and dolphin, and Pcdh15 in Yangochiroptera. In addition, adult echolocating bats have higher levels of Otof expression in the auditory cortex than do their embryos and non-echolocation bats. Cdh23 and Pcdh15 encode the upper and lower parts of tip-links, and both genes show signals of convergent evolution and positive selection in echolocators, implying that they may co-evolve to optimize cochlear amplification. Convergent evolution and expression patterns of Otof suggest the potential role of nerve and brain in echolocation. Our synthesis of gene sequence and gene expression analyses reveals that positive selection, parallel evolution, and perhaps co-evolution and gene expression affect multiple hearing genes that play different roles in audition, including voltage and bundle motility in cochlear amplification, nerve transmission, and brain function
Environmental metabarcoding reveals contrasting belowground and aboveground fungal communities from poplar at a Hg phytomanagement site
Characterization of microbial communities in stressful conditions at a field level is rather scarce, especially when considering fungal communities from aboveground habitats. We aimed at characterizing fungal communities from different poplar habitats at a Hg-contaminated phytomanagement site by using Illumina-based sequencing, network analysis approach, and direct isolation of Hg-resistant fungal strains. The highest diversity estimated by the Shannon index was found for soil communities, which was negatively affected by soil Hg concentration. Among the significant correlations between soil operational taxonomic units (OTUs) in the co-occurrence network, 80% were negatively correlated revealing dominance of a pattern of mutual exclusion. The fungal communities associated with Populus roots mostly consisted of OTUs from the symbiotic guild, such as members of the Thelephoraceae, thus explaining the lowest diversity found for root communities. Additionally, root communities showed the highest network connectivity index, while rarely detected OTUs from the Glomeromycetes may have a central role in the root network. Unexpectedly high richness and diversity were found for aboveground habitats, compared to the root habitat. The aboveground habitats were dominated by yeasts from the Lalaria, Davidiella, and Bensingtonia genera, not detected in belowground habitats. Leaf and stem habitats were characterized by few dominant OTUs such as those from the Dothideomycete class producing mutual exclusion with other OTUs. Aureobasidium pullulans, one of the dominating OTUs, was further isolated from the leaf habitat, in addition to Nakazawaea populi species, which were found to be Hg resistant. Altogether, these findings will provide an improved point of reference for microbial research on inoculation-based programs of tailings dumps
FRW Cosmologies with Adiabatic Matter Creation
Some properties of cosmological models with matter creation are investigated
in the framework of the Friedman-Robertson-Walker (FRW) line element. For
adiabatic matter creation, as developed by Prigogine and coworkers, we derive a
simple expression relating the particle number density and energy density
which holds regardless of the matter creation rate. The conditions to
generate inflation are discussed and by considering the natural
phenomenological matter creation rate , where is a
pure number of the order of unity and is the Hubble parameter, a minimally
modified hot big-bang model is proposed. The dynamic properties of such models
can be deduced from the standard ones simply by replacing the adiabatic index
of the equation of state by an effective parameter . The thermodynamic behavior is determined and it is also
shown that ages large enough to agree with observations are obtained even given
the high values of suggested by recent measurements.Comment: 27 pages, 2 figures (appended as postscript files), uses LATE
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