Characterization of Stem-Like Cells in Mucoepidermoid Tracheal Paediatric Tumor

Stem cells contribute to regeneration of tissues and organs. Cells with stem cell-like properties have been identified in tumors from a variety of origins, but to our knowledge there are yet no reports on tumor-related stem cells in the human upper respiratory tract. In the present study, we show that a tracheal mucoepidermoid tumor biopsy obtained from a 6 year-old patient contained a subpopulation of cells with morphology, clonogenicity and surface markers that overlapped with bone marrow mesenchymal stromal cells (BM-MSCs). These cells, designated as MEi (mesenchymal stem cell-like mucoepidermoid tumor) cells, could be differentiated towards mesenchymal lineages both with and without induction, and formed spheroids in vitro. The MEi cells shared several multipotent characteristics with BM-MSCs. However, they displayed differences to BM-MSCs in growth kinectics and gene expression profiles relating to cancer pathways and tube development. Despite this, the MEi cells did not possess in vivo tumor-initiating capacity, as proven by the absence of growth in situ after localized injection in immunocompromised mice. Our results provide an initial characterization of benign tracheal cancer-derived niche cells. We believe that this report could be of importance to further understand tracheal cancer initiation and progression as well as therapeutic development

Acute renal failure in a middle-aged woman with 2,8-dihydroxyadeninuria

To access publisher full text version of this article. Please click on the hyperlink in Additional Links fieldNo abstract availabl

Muscleworms, \u3ci\u3eParelaphostrongylus andersoni\u3c/i\u3e (Nematoda: Protostrongylidae), Discovered in Columbia White-Tailed Deer from Oregon and Washington: Implications for Biogeography and Host Associations

Parelaphostrongylus andersoni is considered a characteristic nematode infecting white-tailed deer (Odocoileus virginianus). Host and geographic distribution for this parasite, however, remain poorly defined in the region of western North America. Fecal samples collected from Columbia white-tailed deer (O. v. leucurus) in a restricted range endemic to Oregon and Washington, USA, were examined for dorsal-spined larvae characteristic of many protostrongylid nematodes. Multilocus DNA sequence data (internal transcribed spacer 2 and cytochrome c oxidase subunit 1) established the identity and a new record for P. andersoni in a subspecies of white-tailed deer previously unrecognized as hosts. Populations of P. andersoni are now recognized along the basin of the lower Columbia River in Oregon and Washington and from south-central Oregon on the North Umpqua River. Current data indicate a potentially broad zone of sympatry for P. andersoni and Parelaphostrongylus odocoilei in the western region of North America, although these elaphostrongylines seem to be segregated, respectively, in white-tailed deer or in black-tailed and mule deer (Odocoileus hemionus) at temperate latitudes. The geographic range for P. andersoni in white-tailed deer is extended substantially to the west of the currently defined limit in North America, and we confirm an apparently extensive range for this elpahostrongyline. These observations are explored in the broader context of host and geographic associations for P. andersoni and related elaphostrongylines in North American cervids

\u3ci\u3eMuellerius capillaris\u3c/i\u3e Dominates the Lungworm Community of Bighorn Sheep at the National Bison Range, Montana

Lungworm infections are common among bighorn sheep (Ovis canadensis) in North America, and the predominant species reported are Protostrongylus stilesi and P. rushi. The only records of another lungworm species, Muellerius capillaris, infecting bighorns come from South Dakota, USA. At the National Bison Range (NBR), Montana, USA we found that across six sampling periods, 100% of wild bighorn sheep surveyed were passing first-stage dorsal-spined larvae (DSL) which appeared to be consistent with M. capillaris. By contrast, only 39%or fewer sheep were passing Protostrongylus larvae. Using molecular techniques, we positively identified the DSL from the NBR bighorns as M. capillaris. This is the first definitive record of M. capillaris infection in a free-ranging bighorn sheep population outside of South Dakota

Cutaneous infection caused by Paecilomyces lilacinus in a renal transplant patient: treatment with voriconazole

To access publisher full text version of this article. Please click on the hyperlink in Additional Links fieldMoulds belonging to the genus Paecilomyces are rare opportunistic pathogens. About 100 cases have been reported in immunocompromised hosts or in relation to surgical procedures. We describe here a cutaneous infection due to P. lilacinus in a renal transplant patient, which responded to voriconazole treatment

Cutaneous infection caused by Paecilomyces lilacinus in a renal transplant patient: treatment with voriconazole

To access publisher full text version of this article. Please click on the hyperlink in Additional Links fieldMoulds belonging to the genus Paecilomyces are rare opportunistic pathogens. About 100 cases have been reported in immunocompromised hosts or in relation to surgical procedures. We describe here a cutaneous infection due to P. lilacinus in a renal transplant patient, which responded to voriconazole treatment

Sister Miriam Marshall and Sister Sylvia Darling standing outside the Australian Inland Mission Nursing Home, Birdsville, Queensland [transparency] /

Mould spots.; Part of The Reverend Andrew Leslie McKay collection of photographs relating to Inland Australia, 1950-1976.; Sister Miriam Marshall and Sister Sylvia Darling, identified from PIC/9193/484.; Also available in an electronic version via the internet at: http://nla.gov.au/nla.pic-vn4181681; Collection donated by Mrs Lyn McKay, widow of Reverend Les McKay, through their daughter Dr. Judith McKay

Icelandic families with autosomal dominant polycystic kidney disease: families unlinked to chromosome 16p13.3 revealed by linkage analysis

To access publisher full text version of this article. Please click on the hyperlink in Additional Links fieldWe have mainly used 3 highly polymorphic DNA markers, 3'HVR (D16S85), 16AC2.5 (D16S291) and SM7 (D16S283), flanking the PKD1 region on chromosome 16p13.3 to establish linkage status in seven Icelandic families with autosomal dominant polycystic kidney disease (ADPKD). In four families, the disease locus is in the PKD1 region, and three families are "unlinked" to chromosome 16p13.3. In one of the "unlinked" families, the disease locus is excluded from a part of the long arm of chromosome 2, and we support a theory of more than 2 loci being responsible for ADPKD. Our data confirm the location of the locus YNH24 (D2S44) to chromosome 2q13-q24