RESPONSE OF STEVIA (Stevia rebaudiana B.) PLANLET ON ADDITION OF ORGANIC MATERIALS IN SOME CONCENTRATIONS OF MS MEDIUM

This study aims to determine the response of the growth of Stevia (Stevia rebaudiana B.) plantlets in vitro on different MS media and to organic material. This study also aims to find media and organic material formulations as substitutes for growth regulators at the culture stage before acclimatization. The method used in this research is Factorial Complete Randomized Design which consists of 2 factors. The first factor is MS media concentration (½ MS and MS Full). The second factor is several types of organic matter such as young corn extract (150 ml/L), bean sprout extract (150 ml/L), and young coconut water (150 ml/  L). The results showed that explants were able to grow shoots or roots on MS Full media as well as ½ MS media. Besides, the types of organic matter also gave different responses, but from this study, it was related to coconut water and young corn extract both to stimulate the growth and root of stevia plantlets.This study aims to determine the response of the growth of Stevia (Stevia rebaudiana B.) plantlets in vitro on different MS media and to organic material. This study also aims to find media and organic material formulations as substitutes for growth regulators at the culture stage before acclimatization. The method used in this research is Factorial Complete Randomized Design which consists of 2 factors. The first factor is MS media concentration (½ MS and MS Full). The second factor is several types of organic matter such as young corn extract (150 ml / L), bean sprout extract (150 ml / L), and young coconut water (150 ml / L). The results showed that explants were able to grow shoots or roots on MS Full media as well as ½ MS media. In addition, the types of organic matter also gave different responses, but from this study it was related to coconut water and young corn extract both to stimulate the growth and root of stevia plantlets.  Keywords:Stevia, MS Media Concentration, Organic MaterialsÂ

Respon Pertumbuhan Bibit Tebu (Saccharum Officinarum L.) Pada Aplikasi Biostimulan Dari Ekstrak Bawang Merah

Sugarcane (Saccharum officinarum L.) is a plantation commodity that has an important role in the economy in Indonesia. The demand for sugar increases with the increase in population. Therefore efforts to increase sugar production by increasing the productivity of sugarcane, one of which is through the use of high quality seeds. Sugarcane Bud Chip seeds have the potential to produce sugarcane with high yields, but efforts to stimulate seedling growth really need to be increased. The use of Biostimulant from shallot extract needs to be tested on sugarcane seedlings because shallots contain the hormone auxin and vitamin B1 which are very necessary to increase the growth of sugarcane seedlings. Therefore this study aims to determine the effect of shallot extract concentration on the initial growth of sugarcane plants using the bud chip method and determine the best concentration. This study was conducted for 2.5 months and was designed using a non-factorial randomized block design with 5 concentrations of shallot extract including B0 (control/no extract); B1 (20%); B2 (40%); B3 (60%); B4 (80%). The parameters include the number of tillers, plant height, number of leaves, and root length. The results of this study indicated that the shallot extract gave a significantly different response to the growth of sugar cane seedlings, as seen in the parameters of the number of tillers, the number of leaves and root length. From the data obtained, shallot extract at a concentration of 40% was the best concentration for all parameters.  Sugarcane (Saccharum officinarum L.) is a plantation commodity that has an important role in the economy in Indonesia. The demand for sugar increases with the increase in population. Therefore efforts to increase sugar production by increasing the productivity of sugarcane, one of which is through the use of high quality seeds. Sugarcane Bud Chip seeds have the potential to produce sugarcane with high yields, but efforts to stimulate seedling growth really need to be increased. The use of natural Growth Regulatory Substances (PGR) from shallot extract needs to be tested on sugarcane seedlings because shallots contain the hormone auxin and vitamin B1 which are very necessary to increase the growth of sugarcane seedlings. Therefore this study aims to determine the effect of shallot extract concentration on the initial growth of sugarcane plants using the bud chip method and determine the best concentration. This study was conducted for 2.5 months and was designed using a non-factorial randomized block design with 5 concentrations of shallot extract including B0 (control/no extract); B1 (20%); B2 (40%); B3 (60%); B4 (80%). The parameters include the number of tillers, plant height, number of leaves, and root length. The results of this study indicated that the shallot extract gave a significantly different response to the growth of sugar cane seedlings, as seen in the parameters of the number of tillers, the number of leaves and root length. From the data obtained, shallot extract at a concentration of 40% was the best concentration for all parameters

MAKING OF NATURAL PLANT GROWTH REGULATOR (PGR) IN THE TELADAN FARMER'S GROUP OF SUMBERJAMBE DISTRICT, JEMBER, EAST JAVA

Some organic ingredients are known to contain nutrients and natural plant growth regulators (PGR), such as coconut water, young corn, sprouts, banana weevil, bamboo shoots. These materials are also a substrate for multiplying local microorganisms that are good for the soil. Therefore, the purpose of  this activities in the Teladan’s Farmers Group, is to introduce, improve skills and implement local microorganism fermentation technologies (bio activators) using organic materials that contain natural plant growth regulators. This activity uses direct methods and practices for making natural PGR. The training participants attended by 100% participants. Besides, during the extraction and fermentation activities of organic materials, it was seen that participants knew the sequence of procedures for making natural PGR from the results of discussions and questions and answers. The results obtained in the pre-test mean the understanding of natural PGR is 53, while the post-test mean score after implementation is 80. There is a significant increase in participants' knowledge about organic growth regulators.. In practice, about 80% of participants have been able to extract and mix ingredients for the fermentation process. The indicator of successful  the  natural PGR well-fermented material  is characterized by a brown colour and smell sour

Pemanfaatan Keong Mas Untuk Pupuk Organik Cair di Kelompok Tani Podo Tentrem Kecamatan Wuluhan Jember

The golden snail is one of the pests that damage rice plants, this pest has the ability to reproduce quickly, causing the golden snail to become one of the main pests in lowland rice. But the golden snail can be used as a basic material for liquid organic fertilizer (LOF) because it contains protein, Azotobacter, Azospirillum, phosphate solubilizing microbes, Pseudomonas, Staphylococcus, enzymes and the hormone auxin. The effectiveness of the golden snail LOF has been tested on several agricultural crops, one of which is rice. In the Podo Tentrem Farmers Group, these pests are simply thrown away and have not been utilized properly. Whereas the potential of this golden snail can be very high if it is processed into organic fertilizer. Therefore, with this community service activity, it is hoped that it can improve competence, skills and increase the production output of the Podo Tentrem farmer group. This can be measured by the enthusiasm of the participants who were attended by 100% of the invitees, besides that the participants also knew the stages of work and succeeded in making Keong Mas LOF well. In addition, other assessment indicators are using quantitative data on pre-test and post-test scores. Before the counseling was carried out, the pretest value was 50 and after the knowledge training, it increased with an average post-test score of 76. These results can indicate that in theory and practice, on average, participants understand and can make golden snail LOF

THE EFFECTS FERMENTED NATURAL PLANT GROWTH REGULATOR ON PRANCAK 95 TOBACCO (NICOTIANA TABACUM L. VAR PRANCAK 95) ACCLIMATIZATION

Prancak 95 tobacco has the potential to be developed in Indonesia because it has a distinctive aroma and a nicotine content of 2.13%. The quality of this tobacco raw material must be improved and started from the use of quality seeds through in vitro culture techniques. However, several things that must be considered are the acclimatization stages. One of the efforts to increase the growth of seedlings during the acclimation can be done by providing natural plant growth regulators (PGR) from extracts of organic materials such as coconut water, young corn, sprouts, and banana weevil that has been fermented in advance using EM-4 bioactivators . Based on this, the purpose of this study was to determine the optimal concentration of natural growth regulators in the acclimatization of Prancak -95 tobacco. This study uses a non factorial randomized block design with four treatments concentrated solution of natural growth regulators, which include: Control (no treatment), 10; 25; and 50 ml/L. The observed variables included the number of leaves, leaf area, plant height, and root length. Data analysis using Analysis of Variance  (ANOVA) Test and continued with Duncan's Multiple Range Test (DMRT) Test at 95% confidence level to find out the best treatment. Based on the results of research that has been carried out for 60 days of observation, it can be concluded that the application of natural PGR is able to spur the growth of seedlings during the acclimatization period. Spraying application at a concentration of 10 ml / L can increase the number of leaves, leaf area, number of roots and height of Prancak 95 tobacco seedlings

Induksi Kalus Dari Beberapa Kultivar Tanaman Kentang (Solanum tuberosum L.) Dataran Medium Secara In Vitro menggunakan Variasi Konsentrasi 2,4-D

This study aims to determine the concentration of 2,4-D to grow a callus on several potato medium cultivars such as DTO-28 and Desiree. This study uses a completely randomized design factorial. Factors to be tested is the concentration of 2,4-D: 4.53; 9.05;13.58; 18.10 μM and Potato Cultivar: DTO-28 and Desiree. There are a total of 8 treatments with 5 replications. Parameters measured include the time appeared callus, callus fresh weight, percentage of callus formation, as well as the morphology of callus kualitif parameters, such as texture, color, type of callus, the number of shoots and roots. Data observation of callus morphology presented descriptively, while other data were analyzed by analysis of variance (ANOVA). To know the difference between the treatment performed DMRT at 5% level. The results of the study, demonstrate that all explants of cultivar Desiree and DTO-28 capable to forming callus, with watery crumb texture and average white and yellowish green. DTO-28 cultivars are more responsive to 2,4-D because emerge faster callus which 5 DAP. Both cultivars tested were able to produce callus heaviest at 18.10 μM concentrations of 2,4-D. From these results it can be concluded that the concentration of 18.10 μM concentrations of 2,4-D is the best in spurring the growth of callus potatoes DTO-28 and Desiree

Respons Pertumbuhan Bibit Budset Tebu (Saccharum officinarum L.) Varietas VMC 86-550 pada Beberapa Konsentrasi dan Frekuensi Aplikasi Pupuk Organik Cair (POC) Urine Sapi

Sugarcane (Saccharum officinarum L.) is an important plantation commodity as the main raw material for producing sugar consumed in Indonesia. One of the varieties cultivated is VMC 86-550, but one of the technical obstacles to its cultivation is the slow and non-uniform growth of the seeds. Therefore, one effort to maximize the growth of budset seed varieties requires proper nutrition and contains growth-promoting hormones. Therefore, this study aims to determine the response of giving liquid organic fertilizer (LOF) from cow urine to the growth of VMC 86-550 sugarcane seedlings using the budset method. To find out the purpose, this study used a factorial randomized block design with a concentration factor of LOF of cow urine consisting of five levels (0%, 10%, 15%, 20%, and 25%). The second factor is the application frequency which consists of once every 2 weeks and once every 4 weeks, so there were 10 treatments and repeated three times. This study used a completely randomized factorial design and was analyzed using ANOVA if there was a significant influence followed by Duncan Multiple Range Test (DMRT) at a 5% level using SPSS 20 software. Observation variables included plant height, stem diameter, number of leaves, number of tillers, and root length of sugarcane seedlings up to 90 HST. The results showed that the addition of cow urine LOF had an effect on the growth of sugarcane seedlings, both from the concentration and frequency of application, but the combination of the two did not have a significant effect on all observation variables.Sugarcane (Saccharum officinarum L.) is an important plantation commodity as the main raw material for producing sugar consumed in Indonesia. One of the varieties cultivated is VMC 86-550, but one of the technical obstacles to its cultivation is the slow and non-uniform growth of the seeds. Therefore, one effort to maximize the growth of budset seed varieties requires proper nutrition and contains growth-promoting hormones. Therefore, this study aims to determine the response of giving liquid organic fertilizer (LOF) from cow urine to the growth of VMC 86-550 sugarcane seedlings using the budset method. To find out the purpose, this study used a factorial randomized block design with a concentration factor of LOF of cow urine consisting of five levels (0%, 10%, 15%, 20%, and 25%). The second factor is the application frequency which consists of once every 2 weeks and once every 4 weeks, so there were 10 treatments and repeated three times. This study used a completely randomized factorial design and was analyzed using ANOVA if there was a significant influence followed by Duncan Multiple Range Test (DMRT) at a 5% level using SPSS 20 software. Observation variables included plant height, stem diameter, number of leaves, number of tillers, and root length of sugarcane seedlings up to 90 HST. The results showed that the addition of cow urine LOF had an effect on the growth of sugarcane seedlings, both from the concentration and frequency of application, but the combination of the two did not have a significant effect on all observation variables

Respons Pertumbuhan Bibit Budset Tebu (Saccharum officinarum L.) Varietas VMC 86-550 pada Beberapa Konsentrasi dan Frekuensi Aplikasi Pupuk Organik Cair (POC) Urine Sapi

Sugarcane (Saccharum officinarum L.) is an important plantation commodity as the main raw material for producing sugar consumed in Indonesia. One of the varieties cultivated is VMC 86-550, but one of the technical obstacles to its cultivation is the slow and non-uniform growth of the seeds. Therefore, one effort to maximize the growth of budset seed varieties requires proper nutrition and contains growth-promoting hormones. Therefore, this study aims to determine the response of giving liquid organic fertilizer (LOF) from cow urine to the growth of VMC 86-550 sugarcane seedlings using the budset method. To find out the purpose, this study used a factorial randomized block design with a concentration factor of LOF of cow urine consisting of five levels (0%, 10%, 15%, 20%, and 25%). The second factor is the application frequency which consists of once every 2 weeks and once every 4 weeks, so there were 10 treatments and repeated three times. This study used a completely randomized factorial design and was analyzed using ANOVA if there was a significant influence followed by Duncan Multiple Range Test (DMRT) at a 5% level using SPSS 20 software. Observation variables included plant height, stem diameter, number of leaves, number of tillers, and root length of sugarcane seedlings up to 90 HST. The results showed that the addition of cow urine LOF had an effect on the growth of sugarcane seedlings, both from the concentration and frequency of application, but the combination of the two did not have a significant effect on all observation variables.Sugarcane (Saccharum officinarum L.) is an important plantation commodity as the main raw material for producing sugar consumed in Indonesia. One of the varieties cultivated is VMC 86-550, but one of the technical obstacles to its cultivation is the slow and non-uniform growth of the seeds. Therefore, one effort to maximize the growth of budset seed varieties requires proper nutrition and contains growth-promoting hormones. Therefore, this study aims to determine the response of giving liquid organic fertilizer (LOF) from cow urine to the growth of VMC 86-550 sugarcane seedlings using the budset method. To find out the purpose, this study used a factorial randomized block design with a concentration factor of LOF of cow urine consisting of five levels (0%, 10%, 15%, 20%, and 25%). The second factor is the application frequency which consists of once every 2 weeks and once every 4 weeks, so there were 10 treatments and repeated three times. This study used a completely randomized factorial design and was analyzed using ANOVA if there was a significant influence followed by Duncan Multiple Range Test (DMRT) at a 5% level using SPSS 20 software. Observation variables included plant height, stem diameter, number of leaves, number of tillers, and root length of sugarcane seedlings up to 90 HST. The results showed that the addition of cow urine LOF had an effect on the growth of sugarcane seedlings, both from the concentration and frequency of application, but the combination of the two did not have a significant effect on all observation variables

Pelatihan Aklimatisasi Bibit Anggrek Botolan dan Pemanfaatan Limbah Cair Dapur Sebagai Alternatif Nutrisi Tanaman

Bibit anggrek biasanya dijumpai dalam bentuk bibit botolan yang merupakan hasil perkecambahan melalui teknik kultur jaringan tanaman. Agar bisa hidup dan tumbuh di luar botol, maka bibit anggrek harus dikeluarkan. Tetapi tidak hanya sekedar dikeluarkan begitu saja, ada perlakuan khusus agar bibit mampu menjadi bibit yang mampu tumbuh berkembang di luar botol. Teknik tersebut merupakan teknik aklimatisasi. Bagi para kaum hawa yang suka dan hobi tanaman anggrek, tentunya ketrampilan untuk melakukan aklimatisasi anggrek sangat bermanfaat. Selain itu, pengetahuan terhadap limbah cair dapur untuk dimanfaatkan menjadi nutrisi tambahan akan sangat menunjang ketrampilan dalam pemeliharaan bibit anggrek. Limbah dapur rumah tangga diantaranya yaitu air cucian beras, air rebusan kentang, dan air kelapa. Limbah cair ini biasanya dibuang percuma, padahal kandungan senyawa organik dan mineral yang dimiliki dapat dimanfaatkan sebagai alternatif nutrisi tanaman. Pelatihan ini bertujuan untuk meningkatkan kesadaran para wanita/ibu rumah tangga untuk dapat memanfaatkan limbah dapur untuk diaplikasikan sebagai pupuk cair alternatif dan menambah terampilnya ibu-ibu dalam berbudidaya anggrek, harapannya akan berdampak positif bagi kelestarian anggrek, keindahan lingkungan, dan add value bagi ibu-ibu. Dari hasil kegiatan pelatihan ini, dapat disimpulkan bahwa teknis pelaksanaan pelatihan meliputi pemaparan teori, pemberian modul dan praktik langsung sudah cukup efektif memberikan pemahaman dan wawasan baru bagi peserta. Dalam praktiknya, peserta sudah mampu melaksanakan praktik aklimatisasi langsung mulai dari pemilihan bibit, menyiapkan media tanam, transplanting bibit dari botol ke pot, perawatan bibit dengan pupuk cair dan limbah cair dapur

INDUKSI KALUS DARI BEBERAPA KULTIVAR TANAMAN KENTANG (Solanum tuberosum L.) DATARAN MEDIUM SECARA IN VITRO MENGGUNAKAN VARIASI KONSENTRASI 2,4-D

Penelitian ini bertujuan untuk mengetahui konsentrasi 2,4-D yang tepat untuk menumbuhkan kalus pada beberapa kultivar tanaman kentang dataran medium seperti DTO-28 dan Desiree. Penelitian ini menggunakan Rancangan Acak Lengkap faktorial. Faktor yang dicoba adalah konsentrasi 2,4-D: 4,53; 9,05; 13,58; 18,10 μM dan Kultivar Kentang: DTO-28 dan Desiree. Terdapat total 8 perlakuan dengan 5 kali ulangan. Parameter yang diukur meliputi saat muncul kalus, berat segar kalus, persentase terbentuknya kalus, serta parameter kualitif terhadap morfologi kalus, seperti tekstur, warna, jenis kalus, jumlah tunas dan akar. Data hasil pengamatan terhadap morfologi kalus disajikan secara deskriptif, sedangkan data yang lain dianalisis dengan analisis ragam (ANOVA). Untuk mengetahui perbedaan antara perlakuan dilakukan uji DMRT pada taraf 5%.Hasil penelitian (33 HST) menunjukkan bahwa semua semua eksplan dari Kultivar DTO-28 dan Desiree mampu membentuk kalus, dengan tekstur remah berair dan rata-rata berwarna putih dan hijau kekuningan. Kultivar DTO-28 lebih responsif terhadap ZPT 2,4-D dan mampu memunculkan kalus lebih cepat yaitu pada 5 HST. Kedua kultivar yang diujikan mampu menghasilkan kalus terberat pada konsentrasi 18,10 μM 2,4-D.Dari hasil tersebut dapat disimpulkan bahwa konsentrasi 18,10 μM 2,4-D merupakan konsentrasi terbaik dalam memacu pertumbuhan kalus kentang DTO-28 dan Desiree