Effect of Propolis on Experimental Cutaneous Wound Healing in Dogs

This study evaluates clinically the effect of propolis paste on healing of cutaneous wound in dogs. Under general anesthesia and complete aseptic conditions, two full thickness skin wounds (3 cm diameter) were created in each side of the chest in five dogs, one dorsal and one ventral, with 10 cm between them. These wounds were randomly allocated into two groups, control group (10 wounds) and propolis group (10 wounds). Both groups were represented in each dog. The wounds were cleaned with normal saline solution and dressed with macrogol ointment in control group and propolis paste in propolis group, twice daily till complete wound healing. Measurement of the wound area (cm2) was monitored planimetrically at 0, 7, 14, 21, 28, and 35 days after injury. The data were analyzed statistically. The results revealed a significant reduction in the wound surface area in the propolis group after 14 and 21 days compared to control group. The wound reepithelization, contraction, and total wound healing were faster in propolis group than in control group during five weeks of study. In conclusion, propolis paste has a positive impact on cutaneous wound healing and it may be suggested for treating various types of wounds in animals

Aloe vera in dentistry: Current status and future prospects

This review declares the chemical composition, pharmacological actions, clinical applications, adverse reactions and future prospects of Aloe vera (AV) in the field of dentistry. Literature pertinent to Aloe vera in dental practice was explored in databases like PubMed, Scopus and Google Scholar. The relevant literature was reviewed and critically appraised in this review. Aloe vera is a miracle plant that has been applied as herbal therapy for thousands of years due to its multiple health benefits. In dental practice, AV has different applications and it attracts the attention of researchers due to its natural origin, low cytotoxicity, and various biological actions like antimicrobial, immunomodulatory, anti-inflammatory, antioxidant, wound healing, and antineoplastic actions. Therefore, it has been used for treatment of oral lesions, endodontic lesions, periodontal disease, oral wounds, and alveolar osteitis as well as in tissue engineering, reduction of plaque formation, decontamination of Gutta percha, around dental implants and others. AV has been used in treatment and prevention of several oral and dental problems. Nevertheless, it has a lot to offer in the dental practice and future studies are recommended to maximize its beneficial role in the field of dentistr

Značajke višestrukih falangealnih ozljeda uočene magnetskom rezonancijom (MR) u konja za vuču - prikaz slučaja

Magnetic resonance imaging (MRI) is fast becoming the gold standard diagnostic tool for lameness in equines. This case report describes the MRI features of multiple phalangeal disorders in a 9-year-old horse with chronic unilateral forelimb lameness due to severe trauma. A complete case history was taken, and a thorough clinical examination, radiography, ultrasonography and MRI were performed. Radiography and ultrasonography showed limited value. MRI revealed edema of the navicular bursa (NB), distal sesamoidean impar desmitis, DDFT injury at navicular and post-navicular levels, cartilage erosion of the pastern and coffin joints, medial collateral desmitis of the fetlock joint, medial collateral sesamoidean desmitis, and lateral collateral desmitis of the coffin joint. In conclusion, severe trauma may induce multiple phalangeal disorders with severe pain in draft horses, and MRI is superior to other diagnostic imaging modalities, such as radiography and ultrasonography, for diagnosing such cases.Magnetska rezonancija (MR) ubrzano postaje zlatni standard u dijagnostici hromosti u konja. U ovom se radu opisuju značajke MR-a u dijagnostici višestrukih falangealnih ozljeda u devetogodišnjeg konja s kroničnom unilateralnom hromošću prednjih ekstremiteta uzrokovanom teškom traumom. Provedena je detaljna anamneza, temeljiti klinički pregled, rendgenska pretraga, ultrazvučna i MR pretraga. Rendgenska i ultrazvučna pretraga pokazale su ograničenu vrijednost. MR je otkrio edem navikularne burze (NB), distalni sezamoidni dezmitis, DDFT ozljedu na navikularnoj i postnavikularnoj razini, eroziju hrskavice putičnih i kopitnih zglobova, medijalni kolateralni dezmitis skočnog zgloba, medijalni kolateralni sezamoidni dezmitis i lateralni kolateralni dezmitis kopitnog zgloba. Zaključeno je da teška trauma može uzrokovati višestruke falangealne ozljede s jakom boli u konja za vuču, a MR je superioran ostalim metodama slikovne dijagnostike kao što su rendgenska i ultrazvučna pretraga u dijagnostici ovakvih slučajeva

Ultrasonographic Diagnosis of Some Scrotal Swellings in Bulls

Information regarding the use of ultrasonography in the diagnosis of testicular and scrotal affections is scarce in bovine. In the present study, eight bulls suffering with scrotal swellings were examined ultrasonographically. The recorded affections included; ruptured urethra with scrotal swelling, testicular hypoplasia, malignant Sertoli cell neoplasm, hydrocele and scrotal hernia. In case of ruptured urethra, the scrotum appeared as thick hyperechoic skin, the testes showed normal echogenicity and the surrounding tissue showed diffuse anechoic areas separated by hyperechoic threads. The hypoplastic testis was less echogenic, and its rete testis was more echogenic, than the normal one. The neoplastic testis had several anechoic areas, hyperechoic masses and areas of normal testicular echogenicity. In hydrocele, the scrotal skin appeared as clear hyperechoic line with anechoic fluid accumulated in the vaginal cavity of the scrotum and both testes had normal echotexture. In scrotal hernia, a hyperechoic omental loop was seen adjacent to high echogenic testis. In conclusion, ultrasonography is a good tool for differential diagnosis of different scrotal swellings in bulls

Utjecaj osteogeničnih dodataka agregatu mineralnog trioksida na razinu genske ekspresije odontoblastičnih markera nakon zatvaranja pulpe u pasa.

This study investigates the effect of addition of dexamethasone, vitamin D3, or chitosan to mineral trioxide aggregate (MTA) on the gene expression level of dentin sialophosphoprotein (DSPP) and matrix extracellular phosphoglycoprotein (MEPE) after pulp capping in dogs. Pulp exposure was performed in sixty dogs’ teeth. The teeth were classified into 3 equal groups according to the evaluation period. Group 1:7 days, group 2: 21 days and group 3: 60 days. Each group was further subdivided according to the pulp capping material used, into subgroup A: MTA + dexamethasone, subgroup B: MTA + dexamethasone + vitamin D3, subgroup C: MTA + chitosan and subgroup D: MTA. According to the group, the pulps of the capped teeth were removed for analysis of the relative mRNA expression level of DSPP and MEPE using PCR. Statistical analysis of all data was performed. In subgroup A, significant expression was observed of DSPP (P≤0.05) in group 2 up to 18.8 relative fold change while in subgroup B a significant upregulated gene expression of DSPP (P≤0.05) up to 29.4 relative fold change was seen. Significant upregulated DSPP expression (P≤0.05) was recorded in groups 1 and 2 up to 6.9 and 3.6 relative fold change, respectively in subgroup C. In conclusion, dexamethasone, with or without vitamin D3 and chitosan, are synergistic odontogenic inducers with MTA for differentiation of dental pulp cells in dogs. The upregulation of DSPP is a good marker for this differentiation.Istraživan je utjecaj dodatka deksametazona, vitamina D3 ili hitozana agregatu mineralnog trioksida na razinu ekspresije gena za dentin-sijalofosfoprotein (DSPP) i gena za ekstracelularni matriks fosfoglikoprotein nakon zatvaranja pulpe u pasa. Pulpa je bila otvorena na 60 zuba. Ti su zubi bili razvrstani u tri jednake skupine ovisno o trajanju promatranja. Prva skupina bila je promatrana sedam dana, druga skupina 21 dan, a treća skupina 60 dana. Svaka skupina bila je podijeljena u podskupine ovisno o materijalu rabljenom za zatvaranje pulpe. Podskupini A bio je primijenjen agregat mineralnog trioksida + deksametazon, podskupini B bio je primijenjen agregat mineralnog trioksida + deksametazon + vitamin D3, podskupini C agregat mineralnog trioksida + hitozan, a podskupini D samo agregat mineralnog trioksida. Sukladno skupinama, pulpe zatvorenih zuba bile su uzete za analizu relativne ekspresije mRNA za dentin-sijalofosfoprotein i za ekstracelularni matriks fosfoglikoprotein PCR-om. Svi podatci bili su statistički obrađeni. Značajna ekspresija gena za dentinsijalofosfoprotein (P≤0,05) bila je dokazana u podskupini A druge skupine u relativnoj vrijednosti 18,8, dok je u podskupini B značajna ekspresija gena za dentin-sijalofosfoprotein (P≤0,05) bila 29,4 puta veća. Značajno povećana ekspresija DSPP (P≤0,05) u prvoj i drugoj skupini sve do 6,9 odnosno 3,6 puta ustanovljena je u podskupini C. Može se zaključiti da deksametazon (s vitaminom D3 ili bez njega) i hitozan djeluju sinergistički s agregatom mineralnog trioksida kao odontogenični pokretači za diferencijaciju stanica zubne pulpe u pasa. Povećana razina dentin-sijalofosfoproteina dobar je pokazatelj te diferencijacije

Recent Advances in the Management of Foreign Body Syndrome in Cattle and Buffaloes: A Review

Foreign body syndrome (FBS) is a fairly common disease of cattle and buffaloes, especially in the developing countries. This disease is caused by ingestion of indigestible metallic and non-metallic blunt or sharp foreign objects. It is associated with high economic losses and therefore an urgent science-based policy is required to control and manage this syndrome. Indiscriminate feeding habits, feed scarcity, industrialization and mechanization of agriculture are predisposing factors for FBS in bovine and bubaline. The condition is difficult to diagnose solely on the basis of clinical signs and physical examination. However, laboratory diagnosis and imaging techniques like radiography and ultrasonography can be of high diagnostic value in detecting the condition. Anemia, increased packed cell volume, neutrophilia with a left shift, increased total protein, globulin, total bilirubin, Alanine Aminotransferase, Alkaline Phosphatase, Phosphorus and decreased albumin/globulin ratio and Calcium are the common abnormal laboratory findings. Recently, ultrasonography has replaced radiography for diagnosis of FBS in bovine and bubaline due to its availability and accuracy in evaluation of features of the reticulum, detection of penetrating metallic objects, diagnosis and assessment of various sequelae of FBS including; local and diffuse traumatic reticuloperitonitis, reticular, splenic, hepatic, abdominal and thoracic abscesses, diaphragmatic hernia, traumatic pericarditis and pleuropneumonia. Although, FBS is ideally treated with rumenotomy, it can be prevented to a large extent by proper management practices, increasing the awareness among the livestock keepers, oral administration of rumen magnets at the age of one year and reapplication of a new magnet every 4 years in animals at high risk

Utjecaj osteogeničnih dodataka agregatu mineralnog trioksida na razinu genske ekspresije odontoblastičnih markera nakon zatvaranja pulpe u pasa.

This study investigates the effect of addition of dexamethasone, vitamin D3, or chitosan to mineral trioxide aggregate (MTA) on the gene expression level of dentin sialophosphoprotein (DSPP) and matrix extracellular phosphoglycoprotein (MEPE) after pulp capping in dogs. Pulp exposure was performed in sixty dogs’ teeth. The teeth were classified into 3 equal groups according to the evaluation period. Group 1:7 days, group 2: 21 days and group 3: 60 days. Each group was further subdivided according to the pulp capping material used, into subgroup A: MTA + dexamethasone, subgroup B: MTA + dexamethasone + vitamin D3, subgroup C: MTA + chitosan and subgroup D: MTA. According to the group, the pulps of the capped teeth were removed for analysis of the relative mRNA expression level of DSPP and MEPE using PCR. Statistical analysis of all data was performed. In subgroup A, significant expression was observed of DSPP (P≤0.05) in group 2 up to 18.8 relative fold change while in subgroup B a significant upregulated gene expression of DSPP (P≤0.05) up to 29.4 relative fold change was seen. Significant upregulated DSPP expression (P≤0.05) was recorded in groups 1 and 2 up to 6.9 and 3.6 relative fold change, respectively in subgroup C. In conclusion, dexamethasone, with or without vitamin D3 and chitosan, are synergistic odontogenic inducers with MTA for differentiation of dental pulp cells in dogs. The upregulation of DSPP is a good marker for this differentiation.Istraživan je utjecaj dodatka deksametazona, vitamina D3 ili hitozana agregatu mineralnog trioksida na razinu ekspresije gena za dentin-sijalofosfoprotein (DSPP) i gena za ekstracelularni matriks fosfoglikoprotein nakon zatvaranja pulpe u pasa. Pulpa je bila otvorena na 60 zuba. Ti su zubi bili razvrstani u tri jednake skupine ovisno o trajanju promatranja. Prva skupina bila je promatrana sedam dana, druga skupina 21 dan, a treća skupina 60 dana. Svaka skupina bila je podijeljena u podskupine ovisno o materijalu rabljenom za zatvaranje pulpe. Podskupini A bio je primijenjen agregat mineralnog trioksida + deksametazon, podskupini B bio je primijenjen agregat mineralnog trioksida + deksametazon + vitamin D3, podskupini C agregat mineralnog trioksida + hitozan, a podskupini D samo agregat mineralnog trioksida. Sukladno skupinama, pulpe zatvorenih zuba bile su uzete za analizu relativne ekspresije mRNA za dentin-sijalofosfoprotein i za ekstracelularni matriks fosfoglikoprotein PCR-om. Svi podatci bili su statistički obrađeni. Značajna ekspresija gena za dentinsijalofosfoprotein (P≤0,05) bila je dokazana u podskupini A druge skupine u relativnoj vrijednosti 18,8, dok je u podskupini B značajna ekspresija gena za dentin-sijalofosfoprotein (P≤0,05) bila 29,4 puta veća. Značajno povećana ekspresija DSPP (P≤0,05) u prvoj i drugoj skupini sve do 6,9 odnosno 3,6 puta ustanovljena je u podskupini C. Može se zaključiti da deksametazon (s vitaminom D3 ili bez njega) i hitozan djeluju sinergistički s agregatom mineralnog trioksida kao odontogenični pokretači za diferencijaciju stanica zubne pulpe u pasa. Povećana razina dentin-sijalofosfoproteina dobar je pokazatelj te diferencijacije

Bioactivity and pH of Nano-White MTA versus NeoMTATM Plus® and MTA Angelus® as root repair materials: An in vitro study

Objective: This study compared the bioactivity and pH of Nano-white mineral trioxide aggregate (NWMTA), Neo-mineral trioxide aggregate plus (NeoMTA Plus), and Mineral trioxide aggregate angelus (MTA-A) as root repair materials. Methods: A total of 60 discs made from the three materials (20 discs each) were prepared according to the manufacturer’s instructions. These discs were packed into plastic molds and allowed to set before testing. For bioactivity study, ten discs of each material were immersed in Hanks\u27 Balanced Salt Solution (HBSS) for 28 days, and analyzed with scanning electron microscope with energy dispersive X-ray (SEM/EDX). Ten discs of each material were used to assess the pH changes by the pH meter at 3 h, 24 h, 72 h, and 168 h. All data were statistically analyzed. Results: After 28 days of immersing in HBSS, the crystals of Nano WMTA, NeoMTA Plus and MTA were covered with calcium phosphate precipitates with no statistically significant difference (P=0.908). The three tested materials induced alkalization of the deionized water after 3 h of immersion and started to decrease at 3 days continuing until the last test at 7 days. Conclusion: The Nano WMTA, NeoMTA Plus and MTA have similar bioactivity and strong alkalizing activity

Feline aortic thromboembolism: Presentation, diagnosis and treatment outcomes of 15 cats

Background: Feline aortic thromboembolism (FATE) is a fatal disease where a blood clot gets lodged into the aortic trifurcation. Aim: This study describes the diagnosis and treatment outcome of FATE in 15 clinical cases. Methods: Fifteen cats with sudden onset of hindlimb paresis/paralysis, vocalization and pain were admitted to the surgery clinic. A full case history was obtained and clinical, orthopedic, neurologic, radiographic, electrocardiographic and echocardiographic examinations were performed for each cat. Treatment protocol included; daily administration of multiple anticoagulant drugs with different mode of actions and meloxicam for 7 successive days. Prophylactic anticoagulant therapy (Clopidogrel and Acetyle salicylic acid) was continued for six months. All data were statistically analyzed and the correlation between time of admission and treatment outcome was tested using Pearson’s correlation coefficient. Results: The case history and clinical, orthopedic, and neurologic examinations revealed sudden onset of hindlimb paralysis (n=12) or paresis (n=3) associated with vocalization and pain, absence of trauma, cold and pale foot paws of hindlimbs (n=13, 86.7%) or cyanosed hind paws (n=2, 13.3 %), absence of femoral pulsation, shallow and rapid open-mouth respiration (61±8 breaths/min), hypothermia (37.9±0.6°C) and tachycardia (155±12 beats/min) with muffled heart sound in four cats (26.7%). Radiography revealed no abnormalities in the hindlimbs, pelvis and spines, cardiomegaly in 5 cats (33.3%), mild pleural effusion and vascular pattern of the lung in six cats (40%) and Valentine’s heart shape in 4 cats (26.7%). Electrocardiography revealed R-wave< 0.9 mV, prolongation of QRS interval in 5 cats (33.3%) and conduction disturbance in 4 cats (26.7%). Echocardiography was consistent with hypertrophic cardiomyopathy (HCM) in 5 cats (33.3%). A statistically significant (P=0.023) strong negative correlation (r=-0.6) was reported between time of admission with subsequent early treatment and recovery from the clinical signs. The treatment was successful in nine cats (60%) while four cats (26.7) were euthanized and two cats (13.3%) were subjected to hindlimb amputation, at the owners' requests. Conclusion: Clinical signs, radiography, electrocardiography and echocardiography are valuable for diagnosis of FATE. The outcome of multiple anticoagulants therapy depends mainly upon early diagnosis and treatment within the first 6 h from the onset of clinical signs

Evaluation of chromocystoscopy in the diagnosis of cystitis in female donkeys.

Early detection of cystitis in equine is essential to improve the prognosis and outcome of therapy. However, the conventional white light endoscopy is not sufficiently accurate for this purpose. Hence, this study evaluated chromoendoscopy as a recent diagnostic tool for cystitis in female donkeys. For this purpose, 5 apparently normal donkeys (control group) and 5 female donkeys with cystitis (diseased group) were used. Physical and rectal examinations, urine analysis, white light cystoscopy, methylene blue-based chromoendoscopy and histopathology were performed in all animals. Turbid urine exhibiting an alkaline pH and a significant (P = .02) increase in the numbers of RBCs and WBCs was observed in the diseased group compared to the control one. In the control group, white light cystoscopy showed a smooth pale pink glistening mucosa with two openings of the ureters and visible submucosal blood vessels. During chromocystoscopy, faint bluish discoloration of the mucosal surface with clearly visible submucosal blood vessels was detectable. These findings were correlated with the histopathological findings of the biopsies collected from the urinary bladder. In the diseased group, white-light cystoscopy showed clearly visible blood vessels, mildly hyperaemic mucosa in focal or diffuse forms and small vesicle formation. Chromocystoscopy revealed dark bluish oedematous and irregular mucosa either in a focal form or a diffuse form (marbled appearance) with deeply stained submucosal blood vessels. Histopathologically, the urothelium was hyperplastic with squamous metaplasia and the lamina propria was infiltrated with few leukocytes and congested blood vessels. Small bluish dots representing the absorbed methylene blue dye were seen in the inflamed areas against the lightly stained mucosa of the bladder. Severe diffuse necrotic cystitis was also seen with bacterial aggregations on the surface. Gram's staining revealed both gram positive bacilli and Gram positive coccobacilli. In conclusion, chromoendoscopy is a helpful tool for early diagnosis of cystitis in female donkeys and enables targeted biopsies, which improves the prognosis and outcome of therapy