The effect of Halcoholic extract of celery leaves on the delivery rate (fertilization and stillbirths), the number, weight and sex ratio of rat off spring

Objectives: Infertility is one of the most common health problems in the world. Different plants are used in traditional medicine to treat infertility. Celery is rich in flavonoids and vitamins E and C. The present study investigated the effect of hydroalcoholic extract of celery leaves on the delivery rate, number, weight and sex ratio of rat offspring. Materials and Methods: In this experimental study, 45 Wistar rats were randomly divided into three groups of fifteen (five male and 10 female rats per group). The control group received 1 ml of distilled water, and experimental groups received respectively doses of 100 and 200 mg/kg/BW celery extract for five weeks by gavage. During the fifth week each male rat was mated with two female rats from the same group in a separate cage. After a week, every female rat was kept in the separate cages. Females received celery extract during pregnancy and until childbirth. Finally, the number of the delivery females, number, weight and sex ratio of offspring were recorded. The data were analyzed by one-way ANOVA test using SPSS15 software. Results: The number of the delivery rats in the experimental groups was not significantly different than the control group (P>0.05). The number of newborns was significantly increased in each experimental group compared to the control group (P0.05). Conclusions: Celery improved fertility due to have flavonoid and antioxidant activity, and its consumption in both sexes can be useful in the treatment of infertility. © 2014 AENSI Publisher All rights reserved

The effect of hydro-alcoholic extract of celery on male rats in fertility control and sex ratio of rat offspring

BACKGROUND AND OBJECTIVE: Celery (Apium graveolens), in traditional medicine is known as appetite and libido stimulant, and also there has been referred to its effect on miscarriage and catamenia. This plant with known plant compounds could be an option for fertility control. This study was conducted to evaluate the effect of hydro-alcoholic extract of celery on male rats in fertility control and sex ratio of rat offspring. METHODS: In this experimental study, 20 male rats were divided randomly into 4 groups, 5 in each group. Control group received no treatment; Sham group received 1 ml normal saline and the treatment groups 1 and 2 received hydro-alcoholic extract of celery in doses of 100 and 200 mg/Kg/BW respectively for 5 weeks. After mating each male rat with two female rats, the number of delivered mice and the number of offspring and their sex were recorded and compared 30 days after the birth. FINDINGS: There was no significance difference between the experimental and control group (p>0.05). The result showed a significant decrease in the number of offspring in doses of 100 and 200 mg/kg (4.25±0.25 and 4.50±0.32 respectively) compared to the control group (7.11±0.26), also a significant increase in the sex ratio in dose of 100 mg/kg (2.93±0.30) compared to the control group (0.98±0.11) was observed (p<0.05). CONCLUSION: It seems that consumption of celery in male decreases the number of offspring and increases the male genesis; because it does not affect on childbirth. It may be useful safely in pregnancy controlling. © 2014, Babol University of Medical Sciences. All rights reserved

Effect of ointment with cabbage, pomegranate peel, and common plantain on wound healing in male rat

زمینه و هدف: تحقیقات بالینی و تجربی متعدد اثربخشی گیاهان دارویی بر روند ترمیم زخم را به اثبات رسانده اند و انار، بارهنگ و کلم از جمله گیاهان مفید در طب سنتی هستند که برای التیام زخم مورد استفاده قرار گرفته اند؛ لذا این مطالعه با هدف بررسی اثر بخشی کرم با اختلاط گیاهان کلم برگ، پیه سفید انار و بخش های هوایی بارهنگ بر زخم پوستی در موش صحرایی طراحی و اجرا شد. روش بررسی: در این مطالعه تجربی 24 راس موش صحرایی بصورت تصادفی به 4 گروه 6 تایی شامل کنترل (سرم فیزیولوژیک)، شاهد (تحت درمان با پتادین)، کنترل مثبت (تحت درمان با فنی توئین) و گروه تجربی تحت درمان با کرم با اختلاط پیه انار، بارهنگ و کلم برگ تقسیم شدند. پس از ایجاد زخم در شرایط غیرعفونی و یکسان، حیوانات بصورت روزانه تحت درمان قرار گرفتند و در روزهای 3، 6، 9 و 14 پس از ایجاد زخم، درصد بهبودی زخم محاسبه گردید. یافته ها: درصد بهبودی زخم در گروه دریافت کننده کرم درمانی در روز های 3، 6، 9 و 14 نسبت به گروه کنترل افزایش معنی داری نشان داد (

Acute Cold / Restraint Stress in Castrated Rats

Objective: The present study aimed to determine whether castration altered osmotically stimulated vasopressin (VP) release and urinary volume and what is the role of endocrine-stress axis in this process. Materials and methods: Totally 108 mice were studied in two main groups of castrated (n=78) and control (n=30). Each group was extracted by acute cold stress (4◦C for 2h/day), restraint stress (by syringes 60cc 2h/day) and cold/restraint stress. The castrated group was treated in sub groups of testosterone, control (sesame oil as vehicle of testosterone). Propranolol as blocker of sympathetic nervous system was given to both groups of castrated mice and main control. Results: Our results showed that, there is interactions between testosterone and sympathetic nervous system on vasopressin, because urine volume was decreased only in testoctomized mice with cold/restraint and cold stress (P<0.001); propranolol as the antagonist of sympathetic nervous system could block and increase urine volume in castrated mice. This increased volume of urine was due to acute cold stress, not restraint stress (p<0.001). The role of testosterone, noradrenalin (NA) and Vasopressin (VP) in the acute cold stress is confirmed, because testosterone could return the effect of decreased urine volume in control group (P<0.001). Conclusion: Considering the effect of cold/restraint stress on urinary volume in castrated mice shows that there is interaction between sex hormone (testosterone), vasopressin and adrenergic systems

Antispasmodic effects of Citrus aurantium flowers aqueous extract on uterus of non-pregnant rats

Background: Citrus aurantium is a small citrus tree, with scented white flowers. The C. aurantium is used in Asian herbal medicine primarily to treat digestive problems. Objective: The goal of this study is to investigate the effect of C. aurantium flower's aqueous extract on uterine contraction in presence of some known uterus stimulants. Materials and Methods: In experimental study 30 virgin Wistar rats 200-300gr were obtained. After laparatomy, a piece of Uterus was dissected out and mounted in an organ bath (10ml) containing De Jalon (29°C) and contracted by KCl (60mM), oxytocin (10mU/ml) and barium chloride (4mM) then the effect of C. aurantium flower's aqueous extract (1-8 mg/ml) on the uterine contractions was investigated. Uterus was separately incubated with propranolol (1?M), naloxone (1?M) and the role of ß-adrenoceptors, opioid receptors were evaluated. Results: Cumulative concentrations of the extract (1-8 mg/ml) decreased KCl, oxytocin and barium chloride induced uterine contractions, dose-dependently (p<0.001). C. aurantium flower's aqueous extract was unaffected on incubation the tissue with propranolol and naloxone. Conclusion: It seems that the extract induced antispasmodic effect mainly via calcium influx blockade. However, neither ß-adrenoceptors nor opioid receptors were involved. Since the extract has antispasmodic effect on uterus contraction therefore we can suggest that more study will be necessary to relief dysmenorrheal

Effects of aqueous extract of celery (Apium graveolens L.) leaves on spermatogenesis in healthy male rats

Objectives: Nowadays, a lot of attention has been paid to the therapeutic properties of herbs, including evaluation of the effects of these plants on fertility in laboratory animals. Apium graveolens L. (celery) has been widely used in traditional medicine for treatment of various disorders including impotency. Therefore, this study was designed to investigate the effects of aqueous extract of A. graveolens on testicular tissue and spermatogenesis in healthy male rats. Materials and Methods: In this research, 24 apparently healthy male rats were divided into three groups, including eight rats in each. The first group as control received only distilled water 1 ml/animal/day. The second and third groups orally received 100 and 200 mg/kg b.w. of the extract, respectively, for 30 days. The day after the last administration of the extract, the rats were sacrificed, the testes were removed entirely, and the morphometric studies were carried out. Epididymal sperm count and histological studies of testicular tissue were conducted. Results: The comparison between the treated and control groups revealed a remarkable increase in the seminiferous tubules diameter, testes volume (p≤0.001), and the number of spermatogonia, primary spermatocytes and spermatozoa. Furthermore, the increase in the number of spermatids and epididymal weight were only significant at high doses of the extract (p≤ 0.05). Conclusions: The results from this study indicated that administration of celery leaf extract may improve spermatogenesis process and also be useful for some sperm fertility parameters