ER Stress-Induced eIF2-alpha Phosphorylation Underlies Sensitivity of Striatal Neurons to Pathogenic Huntingtin

A hallmark of Huntington's disease is the pronounced sensitivity of striatal neurons to polyglutamine-expanded huntingtin expression. Here we show that cultured striatal cells and murine brain striatum have remarkably low levels of phosphorylation of translation initiation factor eIF2 alpha, a stress-induced process that interferes with general protein synthesis and also induces differential translation of pro-apoptotic factors. EIF2 alpha phosphorylation was elevated in a striatal cell line stably expressing pathogenic huntingtin, as well as in brain sections of Huntington's disease model mice. Pathogenic huntingtin caused endoplasmic reticulum (ER) stress and increased eIF2 alpha phosphorylation by increasing the activity of PKR-like ER-localized eIF2 alpha kinase (PERK). Importantly, striatal neurons exhibited special sensitivity to ER stress-inducing agents, which was potentiated by pathogenic huntingtin. We could strongly reduce huntingtin toxicity by inhibiting PERK. Therefore, alteration of protein homeostasis and eIF2 alpha phosphorylation status by pathogenic huntingtin appears to be an important cause of striatal cell death. A dephosphorylated state of eIF2 alpha has been linked to cognition, which suggests that the effect of pathogenic huntingtin might also be a source of the early cognitive impairment seen in patients

The spin structure of the Lambda hyperon in quenched lattice QCD

It has been suggested to use the production of Lambda hyperons for investigating the nucleon spin structure. The viability of this idea depends crucially on the spin structure of the Lambda. Using nonperturbatively O(a) improved Wilson fermions in the quenched approximation we have studied matrix elements of two-quark operators in the Lambda. We present results for the axial vector current, which give us the contributions of the u, d, and s quarks to the Lambda spin.Comment: Lattice2001(matrixelement), 3 pages, 2 figure

Temperature dependence of J–V and C–V characteristics of n-InAs/p-GaAs heterojunctions prepared by flash evaporation technique and liquid phase epitaxy

In this work, n-type of InAs films have been successfully fabricated on p-GaAs monocrystalline substrates by both flash evaporation technique and liquid phase epitaxy. The elemental composition of the prepared films has been confirmed by energy dispersive X-ray (EDX) spectroscopy. The morphology of the films has been characterized by scanning electron microscopy (SEM). The current transport mechanisms of n-InAs/p-GaAs heterojunctions in the temperature range 300-400 K have been investigated. Temperature-dependent dark current density-voltage (J–V) studies under forward and reverse bias have been carried out for this purpose. In the temperature range studied, the dark current contribution in the low bias range is believed to be due to the generation-recombination of minority carriers in the space-charge region. A change in the preparation technique does not seem to have altered the dark current conduction mechanism. Capacitance-voltage (C–V) at various temperatures has been measured to identify the junction type as well as determination of the important junction parameters

Hard diffraction from parton rescattering in QCD

We analyze the QCD dynamics of diffractive deep inelastic scattering. The presence of a rapidity gap between the target and diffractive system requires that the target remnant emerges in a color singlet state, which we show is made possible by the soft rescattering of the struck quark. This rescattering is described by the path-ordered exponential (Wilson line) in the expression for the parton distribution function of the target. The multiple scattering of the struck parton via instantaneous interactions in the target generates dominantly imaginary diffractive amplitudes, giving rise to an "effective pomeron" exchange. The pomeron is not an intrinsic part of the proton but a dynamical effect of the interaction. This picture also applies to diffraction in hadron-initiated processes. Due to the different color environment the rescattering is different in virtual photon- and hadron-induced processes, explaining the observed non-universality of diffractive parton distributions. This framework provides a theoretical basis for the phenomenologically successful Soft Color Interaction model which includes rescattering effects and thus generates a variety of final states with rapidity gaps. We discuss developments of the SCI model to account for the color coherence features of the underlying subprocesses.Comment: 12 pages, 9 figures, REVTeX4. Somewhat expanded and modified version, two new subsections added. To appear in PR

Tensor interaction constraints from beta decay recoil spin asymmetry of trapped atoms

We have measured the angular distribution of recoiling daughter nuclei emitted from the Gamow-Teller $\beta$ decay of spin-polarized $^{80}$Rb. The asymmetry of this distribution vanishes to lowest order in the Standard Model (SM) in pure Gamow-Teller decays, producing an observable very sensitive to new interactions. We measure the non-SM contribution to the asymmetry to be $A_{T}$= 0.015 $\pm$ 0.029 (stat) $\pm$ 0.019 (syst), consistent with the SM prediction. We constrain higher-order SM corrections using the measured momentum dependence of the asymmetry, and their remaining uncertainty dominates the systematic error. Future progress in determining the weak magnetism term theoretically or experimentally would reduce the final errors. We describe the resulting constraints on fundamental 4-Fermi tensor interactions.Comment: 11 pages, 13 figures; v2 published in Phys. Rev. C, with referee clarifications and figures improved for black-and-whit