A study of method development and validation for estimation of Azelastine hydrochloride in nasal spray formulations by RP-HPLC method

A simple reverse-phase HPLC method for the estimation of Azelastine hydrochloride in nasal spray formulations has been developed. The method is simple, accurate, precise, specific and linear over the analysis range. This developed method has been validated according to International Conference on Harmonization (ICH) guideline with respect to system suitability, specificity, precision, linearity, accuracy, and robustness. An isocratic condition of mobile phase comprising Phosphate buffer (pH 3.1): Acetonitrile in a ratio of 60:40, v/v at a flow rate of 1.0 mL/minute over RP C18 (octadecylsilane (ODS), 250 × 4.6 mm, 5 µm, CHROMOSIL) column at ambient temperature was maintained. Besides, the chromatographic peak was observed sharp & symmetric. The proposed method was successfully applied for the estimation of the Azelastine hydrochloride in nasal spray formulation

Synthesis, characterization and antimicrobial screening of some novel chalcones and their derivatives

1642-1648  Synthesis of some novel 2-(4-(allyloxy)-3-methoxyphenyl)-4H-chromen-4-ones 5a-f and 2-(4-(allyloxy)-3-methoxyphenyl)-3-chloro-4H-chromen-4-ones 6a-f by oxidative cyclisation of (E)-3-(4-allyloxy)-3-methoxyphenyl)-1-(2-hydroxyphenyl)prop-2-en-1-ones using DMSO/I2 and DMSO/CuCl2 at reflux condition has been carried out. A useful and efficient synthetic strategy for the synthesis of 4-allyloxy-3-methoxyphenyl chromones has been reported. The synthesized compounds have been characterized by melting point, FT-IR, NMR and EI-MS spectral data. The synthesized compounds have been evaluated for their antibacterial and antifungal activities. Most of the compounds are found to be of comparable potency when compared with the reference standard drugs

Synthesis and Antimicrobial Activity of New Carbohydrazide Bearing Quinoline Scaffolds in Silico ADMET and Molecular Docking Studies

In search of a more potent and new series of fluorine-containing quinoline, hybrid Schiff bases (6a–o) analogues were synthesized by a facile and efficient conventional method. They were developed via condensation of quinoline-4-carbohydrazide intermediate and aromatic aldehydes in presence of ethanol. All compounds viz., 6a–o were efficiently synthesized in good yields in ranges of 76–84%, respectively. All synthesized compounds were well characterized by using various spectroscopic techniques such as FT-IR, 1H NMR, 13C NMR, Mass spectroscopy. Moreover, all newly synthesized hybrid Schiff bases (6a–o) have been screened for their antifungal and antibacterial activity. Among these compounds (6a–d) shows good antibacterial activity, while compound 6b was found to be effective against a fungal pathogen Aspergillus niger and compound 6a was found to inhibit the visible growth of Staphylococcus aureus ATCC 6538 at low concentration with MIC 340 µg/.</p