Elicitors enhanced the production of bioactive compounds in shoot cultures of Hypericum amblysepalum

In this study, we examined the effects of elicitors MeJA (Methyl jasmonate) and JA (Jasmonic acid) (50, 100, and 200 µM) on the growth and production of 15 phenolic compounds in shoot cultures of Hypericum amblysepalum using the LC-MS/MS method. The biomass production increased on shoots elicited with 50 µM JA and MeJA. However, higher concentrations of these elicitors had a negative effect on the growth of the shoot cultures, while simultaneously resulting in an increase in the secondary metabolite content. The elicitor MeJA (especially 200 µM MeJA) was more effective in terms of increasing the phenolic compound contents. The highest amounts of rutin (2.8 fold), astragalin (2.4 fold), protocatechuic acid (2.4 fold), hesperidin (2 fold), pseudohypericin (1.9 fold), chlorogenic acid (1.4 fold), and hypericin (1.9 fold) were obtained from the shoots elicited with MeJA (200 µM). In addition, the application of elicitor JA 200 µM increased the amount of luteolin (2 fold), quercitrin (1.9 fold), apigetrin (4 fold), apigenin (2.9 fold), and hyperoside (1.3 fold). The current study revealed that specific secondary plant metabolites can be regulated by exogenous elicitors in shoot cultures of H. ambysepalum, thus highlighting their promise as a very valuable source of raw materials for the pharmaceutical industr

Contents of phenolic and flavonoid compounds in Isatis demiriziana Mısırdalı: an endemic to the Southeast

Isatis demiriziana Mısırdalı plant contains a number of compounds which has anticarcinogenic, antioxidant and other preventive effects. In this study, the flavonoid and phenolic contents in the plant samples harvested in the vegetative (leaf and root) and full flowering season (flower, leaf and root) were determined by LC-MS/MS. Among the 27 compounds studied, malic acid was found to be the most abundant compound in the methanolic extracts of samples and the amount of malic acid of vegetative root extracts were the highest (30124,37 µg g-1 dry-extract). Moreover, it was also determined considerable amount of salicylic acids and p-coumaric in the root extracts. This study is the first detailed study on the phenolic and flavonoid compounds of I. demiriziana. Based on the findings of this study, in further researches might be refered as an additional source in production of phenolic and flavonoid compounds