Firms’ Perceptions of the Business Cycle and Their Managerial and Financial Conditions

Perception of the business cycle can differ among firms, which has been confirmed by extensive survey data. The question we want to answer in this paper is "What causes differences in business cycle perception?" by utilizing a newly designed questionnaire. Specifically, we match properties emphasizing the absolute level of economic activities or the direction of their changes and indicators of a firm's managerial and financial conditions, and examine the relationship between them. Based on our results, we could not find a particular indicator for the properties of firms that indicate level or trend change. However, a higher level of capital or number of employees suggests a tendency to emphasize change or rate of change, rather than the level of the business survey index. With regards to the time horizon of the business cycle, profit ratios (profit against sales or total assets) in general are good indicators. Firms with a high debt ratio tend to be myopic. Regression analysis shows that firms with high rates of investment/sales significantly tend to have a long-term vision.business cycle perception, financial indicators, managerial indicators, firm, Regression analysis

The Kinesin-Related Protein, Hset, Opposes the Activity of Eg5 and Cross-Links Microtubules in the Mammalian Mitotic Spindle

We have prepared antibodies specific for HSET, the human homologue of the KAR3 family of minus end-directed motors. Immuno-EM with these antibodies indicates that HSET frequently localizes between microtubules within the mammalian metaphase spindle consistent with a microtubule cross-linking function. Microinjection experiments show that HSET activity is essential for meiotic spindle organization in murine oocytes and taxol-induced aster assembly in cultured cells. However, inhibition of HSET did not affect mitotic spindle architecture or function in cultured cells, indicating that centrosomes mask the role of HSET during mitosis. We also show that (acentrosomal) microtubule asters fail to assemble in vitro without HSET activity, but simultaneous inhibition of HSET and Eg5, a plus end-directed motor, redresses the balance of forces acting on microtubules and restores aster organization. In vivo, centrosomes fail to separate and monopolar spindles assemble without Eg5 activity. Simultaneous inhibition of HSET and Eg5 restores centrosome separation and, in some cases, bipolar spindle formation. Thus, through microtubule cross-linking and oppositely oriented motor activity, HSET and Eg5 participate in spindle assembly and promote spindle bipolarity, although the activity of HSET is not essential for spindle assembly and function in cultured cells because of centrosomes

Genetic Association between Swine Leukocyte antigen Class II Haplotypes and Reproduction Traits in Microminipigs

The effects of swine leukocyte antigen (SLA) molecules on numerous production and reproduction performance traits have been mainly reported as associations with specific SLA haplotypes that were assigned using serological typing methods. In this study, we intended to clarify the association between SLA class II genes and reproductive traits in a highly inbred population of 187 Microminipigs (MMP), that have eight different types of SLA class II haplotypes. In doing so, we compared the reproductive performances, such as fertility index, gestation period, litter size, and number of stillbirth among SLA class II low resolution haplotypes (Lrs) that were assigned by a polymerase chain reaction-sequence specific primers (PCR-SSP) typing method. Only low resolution haplotypes were used in this study because the eight SLA class II high-resolution haplotypes had been assigned to the 14 parents or the progenitors of the highly inbred MMP herd in a previous publication. The fertility index of dams with Lr-0.13 was significantly lower than that of dams with Lr-0.16, Lr-0.17, Lr-0.18, or Lr-0.37. Dams with Lr-0.23 had significantly smaller litter size at birth than those with Lr-0.17, Lr-0.18, or Lr-0.37. Furthermore, litter size at weaning of dams with Lr-0.23 was also significantly smaller than those dams with Lr-0.16, Lr-0.17, Lr-0.18, or Lr-0.37. The small litter size of dams with Lr-0.23 correlated with the smaller body sizes of these MMPs. These results suggest that SLA class II haplotypes are useful differential genetic markers for further haplotypic and epistatic studies of reproductive traits, selective breeding programs, and improvements in the production and reproduction performances of MMPs

Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression

We cloned and characterized two new coral fluorescent proteins: h2-3 and 1-41. h2-3 formed an obligate dimeric complex and exhibited bright green fluorescence. On the other hand, 1-41 formed a highly multimeric complex and exhibited dim red fluorescence. We engineered 1-41 into AzaleaB5, a practically useful red-emitting fluorescent protein for cellular labeling applications. We fused h2-3 and AzaleaB5 to the ubiquitination domains of human Geminin and Cdt1, respectively, to generate a new color variant of Fucci (Fluorescent Ubiquitination-based Cell-Cycle Indicator): Fucci5. We found Fucci5 provided more reliable nuclear labeling for monitoring cell-cycle progression than the 1st and 2nd generations that used mAG/mKO2 and mVenus/mCherry, respectively. Key words: fluorescent protein, cell cycle, time-lapse imaging, flow cytometr

Difference in number of loci of swine leukocyte antigen classical class I genes among haplotypes

International audienceThe structure of the entire genomic region of swine leukocyte antigen (SLA)—the porcine major histocompatibility complex—was recently elucidated in a particular haplotype named Hp-1.0 (H01). However, it has been suggested that there are differences in the number of loci of SLA genes, particularly classical class I genes, among haplotypes. To clarify the between-haplotype copy number variance in genes of the SLA region, we sequenced the genomic region carrying SLA classical class I genes on two different haplotypes, revealing increments of up to six in the number of classical class I genes in a single haplotype. All of the SLA-1(-like) (SLA-1 and newly designated SLA-12) and SLA-3 genes detected in the haplotypes thus analyzed were transcribed in the individual. The process by which duplication of SLA classical class I genes was likely to have occurred was interpreted from an analysis of repetitive sequences adjacent to the duplicated class I genes

Observing System Simulation Experiment to Reproduce Kelvin Wave in the Venus Atmosphere

Planetary-scale 4-day Kelvin-type waves at the cloud top of the Venus atmosphere have been reported from the 1980s, and their significance for atmospheric dynamics has been pointed out. However, these waves have not been reproduced in Venus atmospheric general circulation models (VGCMs). Recently, horizontal winds associated with the planetary-scale waves at the cloud top have been obtained from cloud images taken by cameras onboard Venus orbiters, which could enable us to clarify the structure and roles of Kelvin-type waves. In order to examine this possibility, our team carried out an idealized observing system simulation experiment (OSSE) with a data assimilation system which we developed. The wind velocity data provided by a CCSR/NIES (Center for Climate System Research/National Institute for Environmental Studies) VGCM where equatorial Kelvin-type waves were assumed below the cloud bottom was used as idealized observations. Results show that 4-day planetary-scale Kelvin-type waves are successfully reproduced if the wind velocity between 15° S and 15° N latitudes is assimilated every 6 h at 70 km altitude. It is strongly suggested that the Kelvin-type waves could be reproduced and investigated by the data assimilation with the horizontal wind data derived from Akatsuki ultraviolet images. The present results also contribute to planning future missions for understanding planetary atmospheres

Evaluation of new radio occultation observations among small satellites at Venus by data assimilation

International audienceWe conducted observing system simulation experiments (OSSEs) for radio occultation measurements (RO) among small satellites, which are expected to be useful for future Venus missions. The effectiveness of the observations based on realistic orbit calculations was evaluated by reproduction of the "cold collar", a unique thermal structure in the polar atmosphere of Venus. Pseudo-temperature observations for the OSSEs were provided from the Venus atmospheric GCM in which the cold collar was reproduced by the thermal forcing. The vertical temperature distributions between 40 and 90 km altitudes at observation points were assimilated. The result showed that the cold collar was most clearly reproduced in the case where the temperature field in high-latitudes was observed twice a day, suggesting that the proposed observation is quite effective to improve the polar atmospheric structure at least. Although the cold collar was also reproduced in the OSSEs for Longwave Infrared Camera (LIR) observations, the result seemed unrealistic and inefficient compared to that obtained in the RO OSSEs. The present study shows that the OSSEs can be used to evaluate observation plans and instruments in terms of reproducibility of specific atmospheric phenomena, and applied to future missions targeting planetary atmospheres