4,489 research outputs found
The Railroad Background of the Florida Senatorial Election of 1851
The national schism concerning the state of the Union following the Mexican War affected the character and intensity of political debate on the Florida scene no less than it did that of other areas throughout the nation. In Congress, Jefferson Davis and other eminent defenders of the Southern position gained the ardent support of Florida’s Democratic senior Senator, David L. Yulee. In his advocacy of the principle of the concurrent majority, as applied to the bicameral nature of the national legislature, Yulee was dealt a devastating blow by Webster and Clay. By late July of 1850, the proponents of the “Omnibus Bill” were still hopeful, and remained so until Maryland’s James A. Pearce suddenly allowed Yulee’s parliamentary maneuvers to divide the bill. The “ultras” had won a temporary victory, though the final compromise could not be averted.” In the final tabulation, Yulee was joined by his Whig colleagues from Florida, Senator Jackson Morton and Representative Edward C. Cabell, in opposing the California and District of Columbia slave-trade bills and approving the extension of slavery in the new Territories. Morton and Yulee also joined hands in support of the fugitive slave bill, with Cabell absent or not voting in the House
Wear Resistant Fused Filament Extrusion Head
Aspects of devices in which plastic is extruded for a three dimensional printer. Novel designs for components of a high performance fused filament extrusion head are disclosed within, a novel “heat-break” and wear resistant “feed tube”. The feed tube serves as a path for filament as it is fed from the cold end into the hot end. The heat-break rigidly connects the cold end to the hot end while providing resistance to the flow of heat
A Massachusetts Mechanic in Florida and Mexico, 1847
The growing tension between the United States and Mexico during the early part of 1846 found Florida singularly unenthusiastic for war. For one thing, she had only achieved statehood the year before. Then, too, she still felt the adverse impact not only of the 1837-43 depression, but also - more important - the devastation of the Seminole War. Despite these feelings, when Congress actually declared war on May 13, 1846, there was general support for the cause throughout the state. Ultimately, five Florida companies were called into service and three saw action in Mexico
A Massachusetts Traveller on the Florida Frontier
The following passage is an excerpt from the travel diary of W. T. Allen. Leaving his home in Leominster, Massachusetts, on March 12, 1852, Allen enjoyed a forty-nine day tour of the Eastern portion of the United States. He journeyed by rail and boat to New York City and then by boat to Savannah, Georgia, and Florida. His stay in Florida lasted sixteen days, from March 21st to April 6th, and the account of his experiences on the Florida frontier comprises about fifteen of the fifty-five pages of his diary. After leaving Florida, Allen travelled to Savannah, Macon, Montgomery, Mobile, New Orleans, Cincinnati, Cleveland, Niagara, Albany, New York and Boston, returning to Leominster on April 29th, 1852
An orthologue of bacteroides fragilis NanH is the principal sialidase in tannerella forsythia
Sialidase activity is a putative virulence factor of the anaerobic periodontal pathogen Tannerella forsythia, but it is uncertain which genes encode this activity. Characterization of a putative sialidase, SiaHI, by others, indicated that this protein alone may not be responsible for all of the sialidase activity. We describe a second sialidase in T. forsythia (TF0035), an orthologue of Bacteroides fragilis NanH, and its expression in Escherichia coli. Sialidase activity of the expressed NanH was confirmed by using 2′-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid as a substrate. Biochemical characterization of the recombinant T. forsythia NanH indicated that it was active over a broad pH range, with optimum activity at pH 5.5. This enzyme has high affinity for 2′-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid (Km of 32.9 ± 10.3 μM) and rapidly releases 4-methylumbelliferone (Vmax of 170.8 ± 11.8 nmol of 4-methylumbelliferone min−1 mg of protein−1). E. coli lysates containing recombinant T. forsythia NanH cleave sialic acid from a range of substrates, with a preference for α2-3 glycosidic linkages. The genes adjacent to nanH encode proteins apparently involved in the metabolism of sialic acid, indicating that the NanH sialidase is likely to be involved in nutrient acquisition
From gratitude to service: engagement, influence and impact
Report detailing the Jubilee Centre for Character and Virtues' impact, influence and reach. This report features figures and infographics for the different areas of the Centre’s work to date. Since launching in May 2012, the Jubilee Centre has conducted rigorous research into how character and virtues impact on individuals and society. During this time, the Centre has engaged with the British public in a range of different ways and has sought to make a significant difference to how character and virtues are perceived. This new report, From Gratitude to Service, demonstrates the influence the Centre has achieved on the ground in a short period of time and the wide variety of channels through which the Centre has promoted and applied its research evidence. The report explores all aspects of the Centre’s work including research, influence and engagement with policy, partnerships and international collaborations, its work with schools and the community
Evaluating Roads as Investments: A Primer on Benefit-Cost and Economic-Impact Analysis
Kansas and neighboring states spend billions of dollars on roads. Do the huge expenditures represent good investments? The taxpaying public will never know because public decision makers rarely analyze road projects as investments. A disciplined use of benefit-cost analysis can close this knowledge gap. Roadways constitute an economically vital form of transportation infrastructure that have the potential to contribute to the productivity and economic growth of state economies—if the economic benefits of the roadways exceed their cost. Benefit-cost analysis totals the annual user benefits derived from road projects and compares these benefits with the total costs related to construction. The analysis, therefore, identifies road projects that have an acceptable or unacceptable return on investment. Consistent and appropriate use of benefit-cost analysis could allow states to allocate road spending to only the highest valued projects, thereby helping to assure that taxpayers’ money generates an acceptable return on investment
Latent protein trees
Unbiased, label-free proteomics is becoming a powerful technique for
measuring protein expression in almost any biological sample. The output of
these measurements after preprocessing is a collection of features and their
associated intensities for each sample. Subsets of features within the data are
from the same peptide, subsets of peptides are from the same protein, and
subsets of proteins are in the same biological pathways, therefore, there is
the potential for very complex and informative correlational structure inherent
in these data. Recent attempts to utilize this data often focus on the
identification of single features that are associated with a particular
phenotype that is relevant to the experiment. However, to date, there have been
no published approaches that directly model what we know to be multiple
different levels of correlation structure. Here we present a hierarchical
Bayesian model which is specifically designed to model such correlation
structure in unbiased, label-free proteomics. This model utilizes partial
identification information from peptide sequencing and database lookup as well
as the observed correlation in the data to appropriately compress features into
latent proteins and to estimate their correlation structure. We demonstrate the
effectiveness of the model using artificial/benchmark data and in the context
of a series of proteomics measurements of blood plasma from a collection of
volunteers who were infected with two different strains of viral influenza.Comment: Published in at http://dx.doi.org/10.1214/13-AOAS639 the Annals of
Applied Statistics (http://www.imstat.org/aoas/) by the Institute of
Mathematical Statistics (http://www.imstat.org
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