Thematic Report Three - Asylum, Security and Extremism

This is the final of three Thematic Reports published as part of a CREST-funded project carried out by a team of researchers at City, University of London, and Cranfield University at the Defence Academy of the United Kingdom. With the objective of better understanding Tamil diaspora communities’ attitudes and engagement in the Sri Lankan civil conflict (1983-2009) and its aftermath, the project explores diaspora and refugee communities’ relationship with the changing socio-political environment in the homeland, exploring what shapes and influences processes of radicalisation or moderation among these communities. It recognises that the socio-political circumstances in which these processes develop are often crucial to understanding why a community or individuals within that community abroad act in a certain way; this includes analysis of different scales and levels of engagement, both in home and host countries, as well as different ‘areas’ of engagement, which can include social, economic and political interactions. The three thematic reports produced as part of this project cumulatively build a comprehensive picture of the state of knowledge on political action among diaspora, refugee and asylum populations. The theoretically-informed, literature- and evidence-grounded conclusions arising from these three thematic reports are therefore of relevance beyond the case of Sri Lanka. Thematic Report One discussed how four analytical concepts, diaspora, transnationalism, cosmopolitanism, and translocalism, have come to frame the academic discussion of overseas politics and the potential of these concepts to shed light on the relationship between mobility and political action. Thematic Report Two complements the previous report’s broad conceptual discussion by specifically focusing on an analysis of the context and drivers of political action among diaspora and refugee populations, and engaging with the term ‘refugee politics’. The current paper – the final Thematic Report – analyses the growing ‘securitisation’ of refugees and other forcibly displaced populations and calls for greater consideration of structural vulnerabilities in the forced migration and displacement cycle that increase the risk of radicalisation, extremism and related political behaviours

Thematic Report Two - The Engagement of Refugees in Transnational Politics: Lessons from the Migration, Diaspora and Refugee Studies Literature

This is the second of three CREST-funded Thematic Reports published by a team of researchers at City, University of London, and Cranfield University at the Defence Academy of the United Kingdom, that cumulatively build a comprehensive picture of the state of knowledge on political action among diaspora and refugee populations. The research team is specifically interested in applying the theoretically-informed, literature and evidence grounded conclusions arising from these reports to understand the attitudes towards and involvement in the Sri Lankan civil conflict (1983-2009) and its aftermath by Tamil diaspora communities, but with a broader application. The CREST project is exploring diaspora and refugee communities’ relationship with the changing socio-political environment in the homeland and how this influences processes of radicalisation or moderation. It recognises that the socio-political circumstances in which these processes develop are often crucial to understanding why a community or individuals within that community abroad act in a certain way; this includes analysis of different scales and levels of engagement, both in home countries and in host countries, as well as different ‘areas’ of engagement, which can include social, economic and political interactions. Thematic Report One discussed how four analytical concepts, diaspora, transnationalism, cosmopolitanism, and translocalism, have come to frame the academic discussion of overseas politics and the potential of the concepts to shed light on the relationship between mobility and political action. The current paper – Thematic Report Two – complements the previous report’s broad conceptual discussion by specifically focusing on an analysis of the context and drivers of political action among diaspora and refugee populations, and engaging with the term ‘refugee politics’. It considers if the available diaspora and migration literature sheds light on whether the processes and dynamics of forced migration are likely to generate identifiable forms of political engagement

Thematic Report One - Understanding Transnational Diaspora Politics: A Conceptual Discussion

This is the first of three CREST-funded Thematic Reports published by a team of researchers at City, University of London, and Cranfield University at the Defence Academy of the United Kingdom examining political action among diaspora populations. The purpose of the report is to explore the theoretical and conceptual basis underpinning academic debates on engagement in the politics of conflict and post-conflict by communities living overseas. The research team is interested specifically in the Sri Lankan civil conflict between 1983 and 2009 and its aftermath, and understanding the attitudes towards and involvement in that conflict on the part of Tamil diaspora communities. The CREST project is exploring diaspora communities’ relationship with the changing socio-political environment in the homeland and how this influences processes of radicalisation or moderation. It recognises that the socio-political circumstances in which these processes develop are often crucial towards understanding why a community or individuals within that community abroad act in a certain way; this includes analysis of different scales and levels of engagement, both in home countries and in host countries, as well as different ‘areas’ of engagement, which can range from social to economic to political interactions. The following review is not specific to the Sri Lankan situation; it is rather concerned with how four concepts, diaspora, transnationalism, cosmopolitanism, and translocalism, have come to frame the academic discussion of diaspora or more broadly overseas politics and the potential of the concepts to shed light on the relationship between mobility and political action. It considers the extent to which these concepts are helpful in identifying the rationale behind specific methods of political participation offering critical reflections on the analytical and normative usefulness of these terms

Analisi biomolecolare di ceppi di Canine Distemper Virus (CDV) in furetti domestici (Mustela putorius furo)

L\u2019agente eziologico del cimurro (CDV) appartiene alla famiglia Paramyxoviridae, genere Morbillivirus ed \ue8 causa di una patologia infettiva e contagiosa in canidi, mustelidi e procionidi. Gli Autori, descrivono un caso clinico di cimurro in due furetti domestici vaccinati con un ceppo Onderstepoort avianizzato. e la caratterizzazione biomolecolare del CDV isolato. I furetti presentavano un quadro clinico caratterizzato da dermatite pruriginosa e squamo-purulenta, alla regione del mento, peri-labiale, peri-vulvare ed al condotto uditivo esterno. I soggetti venivano a morte tre settimane dopo l\u2019esordio dei sintomi. I campioni di croste prelevati sono stati sottoposti ad estrazione degli acidi nucleici ed analizzati mediante RT-PCR per CDV. E\u2019 stata effettuata l\u2019analisi con enzimi di restrizione (RFLP-PCR) di conferma, per discriminare i ceppi vaccinali dai ceppi di campo. I prodotti di amplificazione sono stati purificati e sottoposti a sequenziamento. Le sequenze di 1823 nt del gene H del CDV sono state comparate mediante Clustal X con analoghe sequenze di ceppi di campo e ceppi vaccinali disponibili su GeneBank. Sulla base dei dati ottenuti le sequenze sono risultate appartenenti al lineage Europa, ben segregato dal lineage America-1, che raccoglie i principali ceppi vaccinali avianizzati, e differente dal cluster dei ceppi Rockborn-like. Il sequenziamento ha confermato il risultato ottenuto con la RFLP-PCR e la mancanza di relazione con il ceppo vaccinale inoculato. Le due sequenze ottenute, presentando un\u2019omologia nucleotidica elevata per ceppi isolati da cani in Italia, sono risultate differenti dal lineage Wildlife. Nel furetto l\u2019infezione da cimurro ha un esito fatale quasi nel 100% dei casi e viene controllata attraverso l\u2019uso di vaccini. Questo lavoro rappresenta un contributo alla esigua disponibilit\ue0 bibliografica in merito alle infezioni da CDV nei mustelidi ed implementa i dati disponibili sui ceppi virali circolanti. Il metodo diagnostico descritto rappresenta un sistema rapido e sensibile per la diagnosi dell\u2019infezione da cimurro

Complement activation at the motor end-plates in amyotrophic lateral sclerosis

BACKGROUND: Amyotrophic lateral sclerosis (ALS) is a fatal progressive neurodegenerative disease with no available therapy. Components of the innate immune system are activated in the spinal cord and central nervous system of ALS patients. Studies in the SOD1(G93A) mouse show deposition of C1q and C3/C3b at the motor end-plate before neurological symptoms are apparent, suggesting that complement activation precedes neurodegeneration in this model. To obtain a better understanding of the role of complement at the motor end-plates in human ALS pathology, we analyzed post-mortem tissue of ALS donors for complement activation and its regulators. METHODS: Post-mortem intercostal muscle biopsies were collected at autopsy from ALS (n = 11) and control (n = 6) donors. The samples were analyzed for C1q, membrane attack complex (MAC), CD55, and CD59 on the motor end-plates, using immunofluorescence or immunohistochemistry. RESULTS: Here, we show that complement activation products and regulators are deposited on the motor end-plates of ALS patients. C1q co-localized with neurofilament in the intercostal muscle of ALS donors and was absent in controls (P = 0.001). In addition, C1q was found deposited on the motor end-plates in the intercostal muscle. MAC was also found deposited on motor end-plates that were innervated by nerves in the intercostal muscle of ALS donors but not in controls (P = 0.001). High levels of the regulators CD55 and CD59 were detected at the motor end-plates of ALS donors but not in controls, suggesting an attempt to counteract complement activation and prevent MAC deposition on the end-plates before they are lost. CONCLUSIONS: This study provides evidence that complement activation products are deposited on innervated motor end-plates in the intercostal muscle of ALS donors, indicating that complement activation may precede end-plate denervation in human ALS. This study adds to the understanding of ALS pathology in man and identifies complement as a potential modifier of the disease process

Calibration and validation of high frequency coastal radar waves exploiting in-situ observations and modelled data in the south-west Sicily

This paper describes a calibration procedure for a non-optimally configured High Frequency Radar (HFR) for the period 1 April 2021, to 31 March 2022, to assess sea waves characteristics. The HFR system, a 16.5 MHz WEllen RAdar (WERA), is part of an innovative network for monitoring the state of the sea. The system is installed in the western part of Sicily (Italy) where a wave buoy is positioned. HFR data underestimate the spectral significant wave heights (Hm0), in particular for Hm0 > 2 m, highlighting the need for calibration of the HFR system to ensure its optimal performance for operational purposes. The calibration was performed with both in-situ and modelled data provided by the Copernicus Marine Service. The best results were obtained when the buoy data were used as reference. Encouraging results were achieved as demonstrated by the improvement of the quantitative metrics after the calibration. Indeed, the RMSE decreased from 0.60 to 0.36 m; the correlation R increased slightly from 0.86 to 0.88, the slope from 0.48 to 0.8; whereas intercept from 0.11 to 0.31 m. Moreover, waves higher than > 2 m are well reproduced by the calibrated HFR time series with the RMSE decreasing from 1.3 to 0.53 m

Seizure activity and brain damage in a model of focal non-convulsive <i>status epilepticus</i>

Aims: Focal non-convulsive status epilepticus (FncSE) is a common emergency condition that may present as the first epileptic manifestation. In recent years, it has become increasingly clear that de novo FncSE should be promptly treated to improve post-status outcome. Whether seizure activity occurring during the course of the FncSE contributes to ensuing brain damage has not been demonstrated unequivocally and is here addressed. Methods: We used continuous video-EEG monitoring to characterise an acute experimental FncSE model induced by unilateral intrahippocampal injection of kainic acid (KA) in guinea pigs. Immunohistochemistry and mRNA expression analysis were utilised to detect and quantify brain injury, 3-days and 1-month after FncSE. Results: Seizure activity occurring during the course of FncSE involved both hippocampi equally. Neuronal loss, blood-brain barrier permeability changes, gliosis and up-regulation of inflammation, activity-induced and astrocyte-specific genes were observed in the KA-injected hippocampus. Diazepam treatment reduced FncSE duration and KA-induced neuropathological damage. In the contralateral hippocampus, transient and possibly reversible gliosis with increase of aquaporin-4 and Kir4.1 genes were observed 3 days post-KA. No tissue injury and gene expression changes were found 1-month after FncSE. Conclusions: In our model, focal seizures occurring during FncSE worsen ipsilateral KA-induced tissue damage. FncSE only transiently activated glia in regions remote from KA-injection, suggesting that seizure activity during FncSE without local pathogenic co-factors does not promote long-lasting detrimental changes in the brain. These findings demonstrate that in our experimental model, brain damage remains circumscribed to the area where the primary cause (KA) of the FncSE acts. Our study emphasises the need to use antiepileptic drugs to contain local damage induced by focal seizures that occur during FncSE

Bioaccumulation of dioxin-like substances and selected brominated flame retardant congeners in the fat and livers of black pigs farmed within the Nebrodi Regional Park of Sicily.

An observational study was designed to assess the bioaccumulation of polychlorodibenzodioxins (PCDD) and polychlorodibenzofurans (PCDF), dioxin-like polychlorobiphenyls (DL-PCB), and 13 selected polybromodiphenylethers (PBDE) in autochthonous pigs reared in the Nebrodi Park of Sicily (Italy). Perirenal fat and liver samples were drawn from animals representative of three different outdoor farming systems and from wild pigs and then analyzed for the chemicals mentioned previously. The highest concentrations of PCDD + PCDF and DL-PCB were detected in the fat (0.45 and 0.35 pg World Health Organization toxicity equivalents [WHO-TE] per g of fat base [FB], respectively) and livers (12.7 and 3.28 pg WHO-TE per g FB) of the wild group, whereas the free-ranging group showed the lowest levels (0.05 and 0.03 pg WHO-TE per g FB in fat and 0.78 and 0.27 pg WHO-TE per g FB in livers). The sum of PBDE congeners was highest in wild pigs (0.52 ng/g FB in fat and 5.64 ng/g FB in livers) and lowest in the farmed group (0.14 ng/g FB in fat and 0.28 ng/g FB in livers). The contamination levels in fat and livers of outdoor pigs had mean concentration values lower than those levels reported for intensively indoor-farmed animals. In wild pigs, bioaccumulation was associated with their free grazing in areas characterized by bush fires. The results of this study aid to emphasize the quality of the environment as a factor to guarantee food safety in typical processed pig meat products, specifically from outdoor and extensive Nebrodi farming systems

Membranes and synaptosomes used to investigate synaptic GABAergic currents in epileptic patients

Among the most prevalent neurological disorders, epilepsy affects about 1% of the population worldwide. We previously found, using human epileptic tissues, that GABAergic neurotransmission impairment is a key mechanism that drives the pathological phenomena that ultimately lead to generation and recurrence of seizures. Using both a "microtransplantation technique" and synaptosomes preparations from drug-resistant temporal lobe epilepsies (TLEs), we used the technique of two-electrode voltage clamp to record GABA-evoked currents, focusing selectively on the synaptic "fast inhibition" mediated by low-affinity GABA(A) receptors. Here, we report that the use-dependent GABA current desensitization (i.e., GABA rundown, which is evoked by applying to the cells consecutive pulses of GABA, at high concentration), which is a distinguishing mark of TLE, is mainly dependent on a dysfunction that affects synaptic GABA(A) receptors. In addition, using the same approaches, we recorded a depolarized GABA reversal potential in synaptosomes samples from the human epileptic subicula of TLE patients. These results, which confirm previous experiments using total membranes, suggest an altered chloride homeostasis in the synaptic area. Finally, the lack of a Zn2+ block of GABA-evoked currents using the synaptosomes supports the enrichment of "synaptic fast inhibitory" GABA(A) receptors in this preparation. Altogether, our findings suggest a pathophysiological role of low-affinity GABA(A) receptors at the synapse, especially during the fast and repetitive GABA release underlying recurrent seizures