New Proteins Found Interacting with Brain Metallothionein-3 Are Linked to Secretion

Metallothionein 3 (MT-3), also known as growth inhibitory factor (GIF), exhibits a neuroinhibitory activity. Our lab and others have previously shown that this biological activity involves interacting protein partners in the brain. However, nothing specific is yet known about which of these interactions is responsible for the GIF activity. In this paper, we are reporting upon new proteins found interacting with MT-3 as determined through immunoaffinity chromatography and mass spectrometry. These new partner proteins—Exo84p, 14-3-3 Zeta, α and β Enolase, Aldolase C, Malate dehydrogenase, ATP synthase, and Pyruvate kinase—along with those previously identified have now been classified into three functional groups: transport and signaling, chaperoning and scaffolding, and glycolytic metabolism. When viewed together, these interactions support a proposed model for the regulation of the GIF activity of MT-3

Planck 2013 results. XX. Cosmology from Sunyaev–Zeldovich cluster counts

We present constraints on cosmological parameters using number counts as a function of redshift for a sub-sample of 189 galaxy clusters from the Planck SZ (PSZ) catalogue. The PSZ is selected through the signature of the Sunyaev-Zeldovich (SZ) effect, and the sub-sample used here has a signal-to-noise threshold of seven, with each object confirmed as a cluster and all but one with a redshift estimate. We discuss the completeness of the sample and our construction of a likelihood analysis. Using a relation between mass M and SZ signal Y calibrated to X-ray measurements, we derive constraints on the power spectrum amplitude σ8 and matter density parameter Ωm in a flat ΛCDM model. We test the robustness of our estimates and find that possible biases in the Y–M relation and the halo mass function are larger than the statistical uncertainties from the cluster sample. Assuming the X-ray determined mass to be biased low relative to the true mass by between zero and 30%, motivated by comparison of the observed mass scaling relations to those from a set of numerical simulations, we find that σ8 = 0.75 ± 0.03, Ωm = 0.29 ± 0.02, and σ8(Ωm/ 0.27)0.3 = 0.764 ± 0.025. The value of σ8 is degenerate with the mass bias; if the latter is fixed to a value of 20% (the central value from numerical simulations) we find σ8(Ωm/0.27)0.3 = 0.78 ± 0.01 and a tighter one-dimensional range σ8 = 0.77 ± 0.02. We find that the larger values of σ8 and Ωm preferred by Planck’s measurements of the primary CMB anisotropies can be accommodated by a mass bias of about 40%. Alternatively, consistency with the primary CMB constraints can be achieved by inclusion of processes that suppress power on small scales relative to the ΛCDM model, such as a component of massive neutrinos. We place our results in the context of other determinations of cosmologicalparameters, and discuss issues that need to be resolved in order to make further progress in this field

Planck 2013 results. XVI. Cosmological parameters

This paper presents the first cosmological results based on Planck measurements of the cosmic microwave background (CMB) temperature and lensing-potential power spectra. We find that the Planck spectra at high multipoles (ℓ ≳ 40) are extremely well described by the standard spatially-flat six-parameter ΛCDM cosmology with a power-law spectrum of adiabatic scalar perturbations. Within the context of this cosmology, the Planck data determine the cosmological parameters to high precision: the angular size of the sound horizon at recombination, the physical densities of baryons and cold dark matter, and the scalar spectral index are estimated to be θ∗ = (1.04147 ± 0.00062) × 10-2, Ωbh2 = 0.02205 ± 0.00028, Ωch2 = 0.1199 ± 0.0027, and ns = 0.9603 ± 0.0073, respectively(note that in this abstract we quote 68% errors on measured parameters and 95% upper limits on other parameters). For this cosmology, we find a low value of the Hubble constant, H0 = (67.3 ± 1.2) km s-1 Mpc-1, and a high value of the matter density parameter,Ωm = 0.315 ± 0.017. These values are in tension with recent direct measurements of H0 and the magnitude-redshift relation for Type Ia supernovae, but are in excellent agreement with geometrical constraints from baryon acoustic oscillation (BAO) surveys. Including curvature, we find that the Universe is consistent with spatial flatness to percent level precision using Planck CMB data alone. We use high-resolution CMB data together with Planck to provide greater control on extragalactic foreground components in an investigation of extensions to the six-parameter ΛCDM model. We present selected results from a large grid of cosmological models, using a range of additional astrophysical data sets in addition to Planck and high-resolution CMB data. None of these models are favoured over the standard six-parameter ΛCDM cosmology. The deviation of the scalar spectral index from unity isinsensitive to the addition of tensor modes and to changes in the matter content of the Universe. We find an upper limit of r0.002\u3c 0.11 on the tensor-to-scalar ratio. There is no evidence for additional neutrino-like relativistic particles beyond the three families of neutrinos in the standard model. Using BAO and CMB data, we find Neff = 3.30 ± 0.27 for the effective number of relativistic degrees of freedom, and an upper limit of 0.23 eV for the sum of neutrino masses. Our results are in excellent agreement with big bang nucleosynthesis and the standard value of Neff = 3.046. We find no evidence for dynamical dark energy; using BAO and CMB data, the dark energy equation of state parameter is constrained to be w = -1.13-0.10+0.13. We also use the Planck data to set limits on a possible variation of the fine-structure constant, dark matter annihilation and primordial magnetic fields. Despite the success of the six-parameter ΛCDM model in describing the Planck data at high multipoles, we note that this cosmology does not provide a good fit to the temperature power spectrum at low multipoles. The unusual shape of the spectrum in the multipole range 20 ≲ ℓ ≲ 40 was seen previously in the WMAP data and is a real feature of the primordial CMB anisotropies. The poor fit to the spectrum at low multipoles is not of decisive significance, but is an “anomaly” in an otherwise self-consistent analysis of the Planck temperature data

Planck 2013 results. V. LFI calibration

We discuss the methods employed to photometrically calibrate the data acquired by the Low Frequency Instrument on Planck. Our calibration is based on a combination of the orbital dipole plus the solar dipole, caused respectively by the motion of the Planck spacecraft with respect to the Sun and by motion of the solar system with respect to the cosmic microwave background (CMB) rest frame. The latter provides a signal of a few mK with the same spectrum as the CMB anisotropies and is visible throughout the mission. In this data releasewe rely on the characterization of the solar dipole as measured by WMAP. We also present preliminary results (at 44 GHz only) on the study of the Orbital Dipole, which agree with the WMAP value of the solar system speed within our uncertainties. We compute the calibration constant for each radiometer roughly once per hour, in order to keep track of changes in the detectors’ gain. Since non-idealities in the optical response of the beams proved to be important, we implemented a fast convolution algorithm which considers the full beam response in estimating the signal generated by the dipole. Moreover, in order to further reduce the impact of residual systematics due to sidelobes, we estimated time variations in the calibration constant of the 30 GHz radiometers (the ones with the largest sidelobes) using the signal of an internal reference load at 4 K instead of the CMB dipole. We have estimated the accuracy of the LFI calibration following two strategies: (1) we have run a set of simulations to assess the impact of statistical errors and systematic effects in the instrument and in the calibration procedure; and (2) we have performed a number of internal consistency checks on the data and on the brightness temperature of Jupiter. Errors in the calibration of this Planck/LFI data release are expected to be about 0.6% at 44 and 70 GHz, and 0.8% at 30 GHz. Both these preliminary results at low and high ℓ are consistent with WMAP results within uncertainties and comparison of power spectra indicates good consistency in the absolute calibration with HFI (0.3%) and a 1.4σ discrepancy with WMAP (0.9%)

Planck 2013 results. XXI. Power spectrum and high-order statistics of the Planck all-sky Compton parameter map

We have constructed the first all-sky map of the thermal Sunyaev-Zeldovich (tSZ) effect by applying specifically tailored component separation algorithms to the 100 to 857 GHz frequency channel maps from the Planck survey. This map shows an obvious galaxy cluster tSZ signal that is well matched with blindly detected clusters in the Planck SZ catalogue. To characterize the signal in the tSZ map we have computed its angular power spectrum. At large angular scales (ℓ \u3c 60), the major foreground contaminant is the diffuse thermal dust emission. At small angular scales (ℓ \u3e 500) the clustered cosmic infrared background and residual point sources are the major contaminants. These foregrounds are carefully modelled and subtracted. We thus measure the tSZ power spectrum over angular scales 0.17° ≲ θ ≲ 3.0° that were previously unexplored. The measured tSZ power spectrum is consistent with that expected from the Planck catalogue of SZ sources, with clear evidence of additional signal from unresolved clusters and, potentially, diffuse warm baryons. Marginalized band-powers of the Planck tSZ power spectrum and the best-fit model are given. The non-Gaussianity of the Compton parameter map is further characterized by computing its 1D probability distribution function and its bispectrum. The measured tSZ power spectrum and high order statistics are used to place constraints onσ8

Planck 2013 results. XV. CMB power spectra and likelihood

This paper presents the Planck 2013 likelihood, a complete statistical description of the two-point correlation function of the CMB temperature fluctuations that accounts for all known relevant uncertainties, both instrumental and astrophysical in nature. We use this likelihood to derive our best estimate of the CMB angular power spectrum from Planckover three decades in multipole moment, ℓ, covering 2 ≤ ℓ ≤ 2500. The main source of uncertainty at ℓ ≲ 1500 is cosmic variance. Uncertainties in small-scale foreground modelling and instrumental noise dominate the error budget at higher ℓs. For ℓ \u3c 50, our likelihood exploits all Planck frequency channels from 30 to 353 GHz, separating the cosmological CMB signal from diffuse Galactic foregrounds through a physically motivated Bayesian component separation technique. At ℓ ≥ 50, we employ a correlated Gaussian likelihood approximation based on a fine-grained set of angular cross-spectra derived from multiple detector combinations between the 100, 143, and 217 GHz frequency channels, marginalising over power spectrum foreground templates. We validate our likelihood through an extensive suite of consistency tests, and assess the impact of residual foreground and instrumental uncertainties on the final cosmological parameters. We find good internal agreement among the high-ℓ cross-spectra with residuals below a few μK2 at ℓ ≲ 1000, in agreement with estimated calibration uncertainties. We compare our results with foreground-cleaned CMB maps derived from all Planck frequencies, as well as with cross-spectra derived from the 70 GHz Planck map, and find broad agreement in terms of spectrum residuals and cosmological parameters. We further show that the best-fit ΛCDMcosmology is in excellent agreement with preliminary PlanckEE and TE polarisation spectra. We find that the standard ΛCDM cosmology is well constrained by Planck from the measurements at ℓ ≲ 1500. One specific example is the spectral index of scalar perturbations, for which we report a 5.4σ deviation from scale invariance, ns = 1. Increasing the multipole range beyond ℓ ≃ 1500 does not increase our accuracy for the ΛCDM parameters, but instead allows us to study extensions beyond the standard model. We find no indication of significant departures from the ΛCDM framework. Finally, we report a tension between the Planck best-fit ΛCDM model and the low-ℓ spectrum in the form of a power deficit of 5–10% at ℓ ≲ 40, with a statistical significance of 2.5–3σ. Without a theoretically motivated model for this power deficit, we do not elaborate further on its cosmological implications, but note that this is our most puzzling finding in an otherwise remarkably consistent data set

Planck 2013 results. XXX. Cosmic infrared background measurements and implications for star formation

We present new measurements of cosmic infrared background (CIB) anisotropies using Planck. Combining HFI data with IRAS, the angular auto- and cross-frequency power spectrum is measured from 143 to 3000  GHz, and the auto-bispectrum from 217 to 545  GHz. The total areas used to compute the CIB power spectrum and bispectrum are about 2240 and 4400 deg2, respectively. After careful removal of the contaminants (cosmic microwave background anisotropies, Galactic dust, and Sunyaev-Zeldovich emission), and a complete study of systematics, the CIB power spectrum is measured with unprecedented signal to noise ratio from angular multipoles ℓ ~ 150 to 2500. The bispectrum due to the clustering of dusty, star-forming galaxies is measured from ℓ ~ 130 to 1100, with a total signal to noise ratio of around 6, 19, and 29 at 217, 353, and 545  GHz, respectively. Two approaches are developed for modelling CIB power spectrum anisotropies. The first approach takes advantage of the unique measurements by Planck at large angular scales, and models only the linear part of the power spectrum, with a mean bias of dark matter haloes hosting dusty galaxies at a given redshift weighted by their contribution to the emissivities. The second approach is based on a model that associates star-forming galaxies with dark matter haloes and their subhaloes, using a parametrized relation between the dust-processed infrared luminosity and (sub-)halo mass. The two approaches simultaneously fit all auto- and cross-power spectra very well. We find that the star formation history is well constrained up to redshifts around 2, and agrees with recent estimates of the obscured star-formation density using Spitzer and Herschel. However, at higher redshift, the accuracy of the star formation history measurement is strongly degraded by the uncertainty in the spectral energy distribution of CIB galaxies. We also find that the mean halo mass which is most efficient at hosting star formation is log (Meff/M⊙) = 12.6 and that CIB galaxies have warmer temperatures as redshift increases. The CIB bispectrum is steeper than that expected from the power spectrum, although well fitted by a power law; this gives some information about the contribution of massive haloes to the CIB bispectrum. Finally, we show that the same halo occupation distribution can fit all power spectra simultaneously. The precise measurements enabled by Planck pose new challenges for the modelling of CIB anisotropies, indicating the power of using CIB anisotropies to understand the process of galaxy formation

Planck 2013 results. XIX. The integrated Sachs-Wolfe effect

Based on cosmic microwave background (CMB) maps from the 2013 Planck Mission data release, this paper presents the detection of the integrated Sachs-Wolfe (ISW) effect, that is, the correlation between the CMB and large-scale evolving gravitational potentials. The significance of detection ranges from 2 to 4σ, depending on which method is used. We investigated three separate approaches, which essentially cover all previous studies, and also break new ground. (i) We correlated the CMB with the Planck reconstructed gravitational lensing potential (for the first time). This detection was made using the lensing-induced bispectrum between the low-ℓ and high-ℓ temperature anisotropies; the correlation between lensing and the ISW effect has a significance close to 2.5σ. (ii) We cross-correlated with tracers of large-scale structure, which yielded a significance of about3σ, based on a combination of radio (NVSS) and optical (SDSS) data. (iii) We used aperture photometry on stacked CMB fields at the locations of known large-scale structures, which yielded and confirms a 4σ signal, over a broader spectral range, when using a previously explored catalogue, but shows strong discrepancies in amplitude and scale when compared with expectations. More recent catalogues give more moderate results that range from negligible to 2.5σ at most, but have a more consistent scale and amplitude, the latter being still slightly higher than what is expected from numerical simulations within ΛCMD. Where they can be compared, these measurements are compatible with previous work using data from WMAP, where these scales have been mapped to the limits of cosmic variance. Planck’s broader frequency coverage allows for better foreground cleaning and confirms that the signal is achromatic, which makes it preferable for ISW detection. As a final step we used tracers of large-scale structure to filter the CMB data, from which we present maps of the ISW temperature perturbation. These results provide complementary and independent evidence for the existence of a dark energy component that governs the currently accelerated expansion of the Universe

Planck 2013 results. XXVIII. The Planck Catalogue of Compact Sources

The Planck Catalogue of Compact Sources (PCCS) is the catalogue of sources detected in the first 15 months of Planck operations, the “nominal” mission. It consists of nine single-frequency catalogues of compact sources, both Galactic and extragalactic, detected over the entire sky. The PCCS covers the frequency range 30–857 GHz with higher sensitivity (it is 90% complete at 180 mJy in the best channel) and better angular resolution (from 32.88′ to 4.33′) than previous all-sky surveys in this frequency band. By construction its reliability is \u3e80% and more than 65% of the sources have been detected in at least two contiguous Planck channels. In this paper we present the construction and validation of the PCCS, its contents and its statistical characterization