AZT exerts its antitumoral effect by telomeric and non-telomeric effects in a mammary adenocarcinoma model

Limitless replicative potential is one of the hallmarks of cancer that is mainly due to the activity of telomerase. This holoenzyme maintains telomere length, adding TTAGGG repetitions at the end of chromosomes in each cell division. In addition to this function, there are extratelomeric roles of telomerase that are involved in cancer promoting events. It has been demonstrated that TERT, the catalytic component of telomerase, acts as a transcriptional modulator in many signaling pathways. Taking into account this evidence and our experience on the study of azidothymidine (AZT) as an inhibitor of telomerase activity, the present study analyzes the effect of AZT on some telomeric and extratelomeric activities. To carry out the present study, we evaluated the transcription of genes that are modulated by the Wnt/β-catenin pathway, such as c-Myc and cyclin-D1 (Cyc-D1) and cell processes related with their expression, such as, proliferation, modifications of the actin cytoskeleton, cell migration and cell cycle in a mammary carcinoma cell line (F3II). Results obtained after treatment with AZT (600 µM) for 15 passages confirmed the inhibitory effect on telomerase. Regarding extratelomeric activities, our results showed a decrease of 64, 38 and 25% in the transcription of c-Myc, Cyc-D1 and TERT, respectively (p<0.05) after AZT treatment. Furthermore, we found an effect on cell migration, reaching an inhibition of 48% (p<0.05) and a significant passage-dependent increase on cell doubling time during treatment. Finally, we evaluated the effect on cell cycle, obtaining a decline in G0/G1 in AZT-treated cells. These results allow us to postulate that AZT is not only an inhibitor of telomerase activity, but also a potential modulator of extratelomeric processes involved in cancer promotion.Fil: Armando, Romina Gabriela. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Analysis of fumonisin B 1 removal by microorganisms in co-occurrence with aflatoxin B 1 and the nature of the binding process

The objectives of this investigation were to evaluate the ability of Saccharomyces cerevisiae CECT 1891 and Lactobacillus acidophilus 24 to remove fumonisin B1 (FB1) from liquid medium; to determine the nature of the mechanism involved in FB1?microorganism interaction and to analyze whether the presence of aflatoxin B1 (AFB1) interferes with the removal of FB1 and vice versa. The results obtained indicated that: (i) both microorganisms were able to remove FB1 from liquid medium; (ii) the removal was a fast and reversible process; (iii) cell viability was not necessary; (iv) the amount of FB1 removed was both toxin- and microorganism concentration-dependent; (v) the process did not involve chemical modification of FB1 molecules; and (vi) cell wall structural integrity of the microorganisms was required for FB1 removal. Consequently, we propose that the mechanism involved in the removal of FB1 is a physical adsorption (physisorption) of the toxin molecule to cell wall components of the microorganisms. It is highly probable that FB1 and AFB1 co-occur in contaminated foods, since the fungal genera Aspergillus and Fusarium frequently occur simultaneously. Therefore, we analyzed whether the presence of AFB1 interferes with the removal of FB1 by the microorganisms previously evaluated, and vice versa. Studies of co-occurrence of both mycotoxins clearly showed that they did not compete for binding sites on the microorganism cell wall and the presence of one toxin did not modify the efficiency of the organism in the removal of the other mycotoxin. These findings may be useful for optimization of mycotoxin binding and provide an important contribution to research on microorganisms with ability to remove these secondary metabolites.Fil: Pizzolitto, Romina Paola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Salvano, Mario Armando. Universidad Nacional de Río Cuarto; ArgentinaFil: Dalcero, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; Argentin

New drugs are not enough-drug repositioning in oncology: An update

Drug repositioning refers to the concept of discovering novel clinical benefits of drugs that are already known for use treating other diseases. The advantages of this are that several important drug characteristics are already established (including efficacy, pharmacokinetics, pharmacodynamics and toxicity), making the process of research for a putative drug quicker and less costly. Drug repositioning in oncology has received extensive focus. The present review summarizes the most prominent examples of drug repositioning for the treatment of cancer, taking into consideration their primary use, proposed anticancer mechanisms and current development status.Fil: Armando, Romina Gabriela. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mengual Gómez, Diego Luis. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Immunoglobulin Treatment in Polymyositis and Dermatomyositis

Polymyositis (PM) and dermatomyositis (DM) are systemic autoimmune diseases of unknown aetiology in which the skeletal muscles are the main targets. Despite the improvement obtained in the last years with new therapeutic options, their prognosis remains poor, with higher rates of morbidity and mortality. Due to the rarity of the disease, few well-designed studies have been published and, to the best of our knowledge, only five randomised controlled trials. The low incidence of the disease, the characteristic relapsing/remitting or chronic and persistently active course, the lack of agreed standardised criteria for diagnosis and for assessment of disease activity makes difficult to carry and to compare studies. Among the treatment options, the use of intravenous immunoglobulin (IVIg) is still matter of debate. In this chapter we describe the use of IVIg in PM and DM, revising the literature and reporting our experience. We analyse our series of 74 subjects with PM or DM diagnosed according to the Bohan and Peter criteria. Usually, IVIg is given in case of refractory or relapsed disease, steroid resistance or dependency. In a previous revision of our data related to 63 patients, we documented that subjects treated with IVIg achieve a clinical and functional remission in a high percentage of cases, that is maintained at long follow-up period (a mean of five years), as compared to control group. The revision of the literature and our data confirm that IVIg is effective alone or as add on therapy in the treatment of inflammatory myopathies, even in refractory or relapsed disease. Most of the patients with PM or DM receive an immunosuppressant such as cyclosporine A (CSA) or mycophenolate mofetil (MMF). We want to verify if the use of IVIg as add-on treatment with CSA or MMF could improve the outcome or could reduce the rate of side effects that are usually linked to the immunosuppressant. We thus revise the literature and our data related to the use of CSA and MMF in PM or DM. The subcutaneous administration (SCIg) could be considered as an alternative to IVIg. In primary immunodeficiency, SCIg has been demonstrated to be linked to a lower incidence of adverse reactions, with reliable efficacy and improvement of the quality of life of treated subjects. We have been the first to publish about a series of seven patients with autoimmune myopathies treated with SCIg. We could document the feasibility and the good tolerance of SCIg, with relevant improvement of the clinical and laboratory features. We here present data related to a larger series. Finally, we review the data related to the mechanisms of action of immunoglobulin administration in autoimmune mediated diseases, in particular underlying the different proposed mechanism of IVIg and SCIg

Telomerase and telomere: their structure and dynamics in health and disease

La telomerasa es la enzima responsable del mantenimiento de la longitud de los telómeros mediante la adición de secuencias repetitivas ricas en guanina, y su actividad se observa principalmente en gametos, células madre y células tumorales. En las células somáticas humanas el potencial de proliferación es limitado, alcanzando la senescencia luego de 50-70 divisiones celulares, debido a que la ADN polimerasa no es capaz de copiar el ADN en los extremos de los cromosomas. Por el contrario, en la mayoría de las células tumorales el potencial de replicación es ilimitado debido al mantenimiento de la longitud telomérica dado por la telomerasa. Los telómeros tienen proteínas adicionales que regulan la unión de la telomerasa. De la misma manera la telomerasa también se asocia con un complejo de proteínas que regulan su actividad. Este trabajo se centra en la estructura y función del complejo telómero/telomerasa y a cómo las alteraciones en su comportamiento conducen al desarrollo de diversas enfermedades, principalmente cáncer. El desarrollo de inhibidores del sistema telómero / telomerasa podría ser un blanco con posibilidades prometedorasTelomerase is the enzyme responsible for the maintenance of telomere length by adding guanine-rich repetitive sequences. Its activity can be seen in gametes, stem cells and tumor cells. In human somatic cells the proliferative potential is limited, reaching senescence after 50-70 cell divisions, because the DNA polymerase is not able to copy the DNA at the ends of chromosomes. By contrast, in most tumor cells the replicative potential is unlimited due to the maintenance of the telomeric length given by telomerase. Telomeres have additional proteins that regulate the binding of telomerase, likewise telomerase associates, with a protein complex that regulates its activity. This work focuses on the structure and function of the telomere/telomerase complex and how changes in its behavior lead to the development of different diseases, mainly cancer. Development of inhibitors of the telomere/telomerase complex could be a target with promising possibilities.Fil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Armando, Romina Gabriela. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Farina, Hernán Gabriel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Telomerase as a cancer target. Development of new molecules

Telomeres are the terminal part of the chromosome containing a long repetitive and non-codifying sequence that has as function protecting the chromosomes. In normal cells, telomeres lost part of such repetitive sequence in each mitosis, until telomeres reach a critical point, triggering at that time senescence and cell death. However, in most of tumor cells in each cell division a part of the telomere is lost, however the appearance of an enzyme called telomerase synthetize the segment that just has been lost, therefore conferring to tumor cells the immortality hallmark. Telomerase is significantly overexpressed in 80?95% of all malignant tumors, being present at low levels in few normal cells, mostly stem cells. Due to these characteristics, telomerase has become an attractive target for new and more effective anticancer agents. The capability of inhibiting telomerase in tumor cells should lead to telomere shortening, senescence and apoptosis. In this work, we analyze the different strategies for telomerase inhibition, either in development, preclinical or clinical stages taking into account their strong points and their caveats. We covered strategies such as nucleosides analogs, oligonucleotides, small molecule inhibitors, G-quadruplex stabilizers, immunotherapy, gene therapy, molecules that affect the telomere/telomerase associated proteins, agents from microbial sources, among others, providing a balanced evaluation of the status of the inhibitors of this powerful target together with an analysis of the challenges ahead.Fil: Mengual Gómez, Diego Luis. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Armando, Romina Gabriela. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cerrudo, Carolina Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular. Área Virus de Insectos; ArgentinaFil: Ghiringhelli, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular. Área Virus de Insectos; ArgentinaFil: Gomez, Daniel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentin

Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains

The development of new strategies for achieving stable asymmetric membrane models has turned interleaflet lipid asymmetry into a topic of major interest. Cyclodextrin-mediated lipid exchange constitutes a simple and versatile method for preparing asymmetric membrane models without the need for sophisticated equipment. Here we describe a protocol for preparing asymmetric supported lipid bilayers mimicking membrane rafts by cyclodextrin-mediated lipid exchange and the main guidelines for obtaining structural information and quantitative measures of their mechanical properties using Atomic force microscopy and Force spectroscopy; two powerful techniques that allow membrane characterization at the nanoscale.Fil: Vazquez, Romina Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Ovalle García, Erasmo. Universidad Nacional Autónoma de México; MéxicoFil: Antillón, Armando. Universidad Nacional Autónoma de México; MéxicoFil: Ortega Blake, Iván. Universidad Nacional Autónoma de México; MéxicoFil: Muñoz Garay, Carlos. Universidad Nacional Autónoma de México; MéxicoFil: Maté, Sabina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentin

Binding of Aflatoxin B1 to Lactic Acid Bacteria and Saccharomyces cerevisiae in vitro: A Useful Model to Determine the Most Efficient Microorganism

Mycotoxins are toxic fungal metabolites found as contaminants in many agricultural products. Feeds contaminated with mycotoxins have a health risk to animals and, as a consequence, may cause big economical losses due to the low efficacy of animal husbandry (Richard, 2007). In addition, directly or indirectly (animal by-products) contaminated foods may also have a health risk to humans (CAST, 2003; Hussein & Brasel, 2001; Wild, 2007). Aflatoxins (AFs), a group of potent mycotoxins with mutagnic, carcinogenic, teratogenic, hepatotoxic and immunosupresive properties, are of particular importance because of their major occurrence and adverse effects on animal and human health, generalized as ?aflatoxicosis? (CAST, 2003; Hussein & Brasel, 2001; Magnoli et al., 2011). The AFs are produced by genus Aspergillus, mainly A. flavus, A. parasiticus and A. nomius, that grow on a variety of raw material during growth, harvest, storage and transportation of for example, the cereal used in the preparation of food and feed commodities (Ito et al., 2001; Kurtzman et al., 1987; Payne, 1998; Pereyra et al., 2010). The investigation of strategies to prevent the presence of AFs in foods, as well as, to eliminate, inactivate or reduce the bio-availability of these mycotoxins in contaminated products include physical, chemical, and biological methods (Bueno et al., 2001; CAST, 2003; Kabak et al., 2006). Limitations such as the loss of nutritional and sensory qualities of the product, the expensive equipment required for these techniques and the impossibility to guarantee the desired results, have allowed us to consider the hipothesis that foods and feeds can always be potentially contaminated with aflatoxins. For instance, in the poultry industry aflatoxin B1 (AFB1) is almost an unavoidable feed contaminant and levels from 0-200 ng/g have been reported (Dalcero et al., 1997). On the other hand, it is known that lactic acid bacteria (LAB) and some yeast, principally Saccharomyces cerevisiae, are capable to bind AFs in liquid media, apparently to cell wall components, polysaccharides and peptidoglycans of LAB (Haskard et al., 2001; Latinen et al., 2004) and glucomannans of yeast (Karaman et al., 2005; Raju & Devegowda 2000) and therefore could be used as potential mycotoxin decontaminating (Armando et al 2011; El-Nezami et al., 1998; Haskard et al., 2000, 2001; Hernandez-Mendoza et al., 2009; Lee et al., 2003; Peltonen et al., 2001; Shetty et al., 2007). The inclusion of appropriate microorganisms in the contaminated diet could prevent the absorption of mycotoxins during their passage in the gastrointestinal tract and eliminated in the faeces (Bueno et al., 2007; El-Nezami et al., 2000; Gratz et al., 2004; Gratz et al., 2007). Moreover, Kankaanpää et al. (2000) showed that the binding of AFB1 to the surface of LAB reduced their adhesive properties, and the accumulation of aflatoxins in the intestine may therefore be reduced via the increased excretion of an aflatoxin-bacteria complex. These considerations encouraged the recent emphasis on biological methods, but mainly focused on preventing AFs absorption in the gastrointestinal tract of the consumers, including these microorganisms in the diet and so prevent the aflatoxicosis effects. The first step in this direction is the selection of the most efficient microorganism for AFB1 removing and while many researchers have assayed LAB and yeast with AFB1 binding abilities (Ciegler et al., 1966; El-Nezami et al., 1998; Gourama & Bullerman, 1995; Haskard et al., 2001; Line et al., 1994; Oatley et al., 2000) no clear mechanism for this effect has been provided. Thus, this selection frequently is performed using a single concentration of AFB1, but we demonstrated that the microorganism efficiency may change when the mycotoxin concentration is modified (Bueno et al., 2007; Pizzolitto, 2011), therefore the microorganism selected could not be the most competent. In this context, we investigated the nature of the interaction between different microorganisms and AFB1 molecule, which led us to develop a model to explain the binding of AFB1 by LAB and Saccharomyces cerevisiae strains. This model allows an estimation of two important parameters related to a microorganism´s capacity for dietary decontamination: the number of binding sites for AFB1 in the surface microorganism (M) and the equilibrium constant of the process involved (Keq), both of them are useful in the selection of the most suitable microorganism in a wide range of AFB1 concentration (Bueno et al., 2007). In adittion, studies of viability of the microorganisms in the salivary and gastrointestinal tract, cell adhesion, autoaggregation, coaggregation and antimicrobial activity against pathogen strains, were also evaluated as a way to research potential beneficial properties on the host (Armando et al., 2011). Thus, in this chapter we describe the development and application of an in vitro methodology to evaluate the aflatoxin B1 binding ability, gastrointestinal tolerance and potential beneficial properties of Saccharomyces cerevisiae strains, useful to select the more appropriated microorganism to be assayed in expensive, complicated but necessary in vivo studies.Fil: Pizzolitto, Romina Paola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Bueno, Dante Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Armando, María Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Salvano, Mario Armando. Universidad Nacional de Río Cuarto; Argentin

Informe técnico de experiencia de fertilización en el cultivo de trigo Campaña 2021

En la región semiárida el agua disponible para los cultivos es una de las principales determinantes del rendimiento, por lo tanto, es posible de esperar que el uso de tecnologías que permitan mejorar la eficiencia de utilización de la misma se traduzca en una mejora de la producción de grano. La selección de genotipos adecuados y el uso de fertilizantes son tecnologías que deben ajustarse a cada condición para poder hacer un uso adecuado y eficiente de las mismas; la finalidad de este informe es poder mostrar resultados obtenidos en lotes de productores donde se incorpora el uso de fertilizantes a partir de una actividad de Investigación Acción Participativa entre entidades públicas y privadasAER General AchaFil: Leonhardt. Diego Armando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil. Agencia de Extensión Rural General Acha; ArgentinaFil: Angolani, Daniel Hugo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil. Agencia de Extensión Rural General Acha; ArgentinaFil: Herrera, Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil. Agencia de Extensión Rural General Acha; ArgentinaFil: Fernandez, Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentin

Reproductive biology of Boana pulchella and Boana punctata (Anura: Hylidae) in sympatric populations of temperate wetlands

Boana pulchella habita principalmente climas templados y B. punctata principalmente climas tropicales, lo que permitiría suponer la presencia de estrategias reproductivas diferentes, aún en la región en la que sus poblaciones son simpátricas. En el presente estudio se analizaron y compararon los ciclos reproductivos, parámetros reproductivos y desarrollo de cuerpos grasos (CG) de Boana pulchella y B. punctata en humedales templados del río Paraná Medio. Boana pulchella presentó óvulos maduros de marzo a diciembre. En machos no hubo variación del volumen testicular a lo largo del año y el mayor desarrollo de CG de los machos se observó en invierno. Esta especie presentó un patrón reproductivo continuo. La vitelogénesis de B. punctata se inició en enero, alcanzando el mayor desarrollo ovárico en febrero (verano) y decreciendo hacia mayo (otoño). Los machos presentaron crecimiento testicular en verano, decreciendo hacia otoño y los CG presentaron su máximo desarrollo en enero para ambos sexos. Esta especie mostró un patrón reproductivo continuo. Ambas especies presentaron dimorfismo sexual con hembras que poseen una relación proporcional entre la talla y la inversión reproductiva. Boana pulchella presentó un mayor esfuerzo reproductivo (ER = 20,48 ± 12,9) y factor de tamaño ovárico (FTO = 25,73) que B. punctata (ER = 10,06 ± 4,45; FTO = 8,99). También se encontraron diferencias interespecíficas en la masa ovárica, complemento ovárico y volúmen testicular, siendo estas variables proporcionalmente mayores en B. pulchella. Las diferencias observadas en las poblaciones simpátricas podrían relacionarse con el desarrollo de estrategias reproductivas bajo diferentes presiones evolutivasBoana pulchella mainly inhabits temperate climates while B. punctata mainly inhabits tropical climates, which would allow assuming the presence of different reproductive strategies, even in the region in which their populations are sympatric. In the present study, we analyzed and compared the reproductive cycles, reproductive parameters, and fat bodies development of Boana pulchella and B. punctata inhabiting in sympatry in temperate wetlands of the Middle Paraná River. Boana pulchella presented mature ovaries from March to December. There was no seasonal variation in testicular size of males, and their fat bodies development was greater during winter. This species exhibited a continuous breeding pattern. The vitellogenesis of B. punctata began in January (summer), reaching higher ovarian development in February (summer) and decreasing to May (autumn). Testicles of males grew in summer and decline in autumn. Fat bodies presented their developmental peak in January for both sexes. This species exhibited a prolonged breeding season. Both species showed sexual dimorphism with larger females having a greater inversion in reproduction. Boana pulchella showed greater reproductive effort (RE = 20.48 ± 12.9) and ovarian size factor (OSF = 25.73) than B. punctata (RE = 10.06 ± 4.45; OSF = 8.99). We also found interspecific differences in ovarian mass, ovarian complement, and testicular volume, these variables being proportionally greater in B. pulchella. Observed differences in sympatric populations could reside in the development of reproductive strategies under different evolutionary pressures.Fil: Antoniazzi, Carolina Elizabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina. Universidad Católica de Santa Fe; ArgentinaFil: López, Javier Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales; ArgentinaFil: Ghirardi, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina. Universidad Católica de Santa Fe; ArgentinaFil: Armando, Andrea Paola. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales; Argentin