A mitochondrial DNA phylogeny of the Leptodactylus fuscus species-complex (Leptodactylidae: Anura

Leptodactylus fuscus is a broadly distributed frog species in the Neotropical region. Previous allozymic evidence supported the hypothesis of L. fuscus as a composite of several species. However, morphological and advertisement call data supported the hypothesis of a single-species taxon. These competing hypotheses were evaluated using cladistic analyses of mitochondrial DNA sequences. Fragments of 12S rRNA, 16S rRNA, and ND1 protein-coding genes from 17 populations were sequenced. Phylogenetic analyses were performed using: maximum parsimony, maximum likelihood, and Bayesian analysis. Data sets were analyzed separately and combined. Analyses of the combined data set show four exclusive clades of L. fuscus: 1) J. V. Gonzalez, 2) Panama, 3) Bolivia and Embarcacion, and 4) the rest of the populations. These data support the hypothesis of multiple species within L. fuscus

Predation of Liolaemus huacahuasicus (Squamata: Iguania: Liolaemini) by Brachistosternus intermedius (Scorpiones: Bothriuridae) in Cumbres Calchaquies, Tucuman Province, Northwestern Argentina

In the course of a herpetological survey of the summit of the Cumbres Calchaquíes Mountains, on a rocky outcrop facing south east, near Provincial Road352 (26º22'45.7”S, 65º43'54.7”W, 3612 m),38.3 km W Hualinchay, Trancas Department, Tucumán Province, on 16 December 2009 at ca. 1500, we found an adult male scorpion (Brachistosternus intermedius) under a rock holding with the chelae and eating the remains of a neonate lizard (posterior midbody, missing tip of tail).Fil: Perez, Cristian Hernan Fulvio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Avila, Luciano Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Camargo, Arley. University Brigham Young; Estados Unido

Predation of Liolaemus huacahuasicus (Squamata: Iguania: Liolaemini) by Brachistosternus intermedius (Scorpiones: Bothriuridae) in Cumbres Calchaquies, Tucuman Province, Northwestern Argentina

In the course of a herpetological survey of the summit of the Cumbres Calchaquíes Mountains, on a rocky outcrop facing south east, near Provincial Road352 (26º22'45.7”S, 65º43'54.7”W, 3612 m),38.3 km W Hualinchay, Trancas Department, Tucumán Province, on 16 December 2009 at ca. 1500, we found an adult male scorpion (Brachistosternus intermedius) under a rock holding with the chelae and eating the remains of a neonate lizard (posterior midbody, missing tip of tail).Fil: Perez, Cristian Hernan Fulvio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Avila, Luciano Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Camargo, Arley. University Brigham Young; Estados Unido

Morphometric differentiation and sexual dimorphism in Limnomedusa macroglossa (Duméril & Bibron, 1841) (Anura: Alsodidae) from Uruguay

Intersexual morphological differences within a species occur in many traits, including body size and shape. Many processes that cause geographic variability in morphology have been proposed: population structure, phenotypic plasticity (environmental effects on development), and natural and/or sexual selection. Several hypotheses can explain patterns of sexual dimorphism in anurans, including natural or intra/inter-sexual selection, and differences in life history strategies between sexes. Limnomedusa macroglossa is considered a habitat specialist restricted to rocky outcrops in Brazil, Argentina, Paraguay, and Uruguay. We evaluated the extent of sexual (size and shape) dimorphism in L. macroglossa from Uruguay based on morphometrics and secondary sexual characteristics, while taking into account geographic variation. Sexual dimorphism in body size of adults was found, but multivariate analyses did not demonstrate the existence of significant differences in shape. There were also significant differences in body size and hind leg measurements among six hydrographic basins as a result from the phenotypic plasticity correlated with local temperature, representing a clinal variation along the latitudinal gradient of Uruguay. The sexual dimorphism found in body size is probably the consequence of higher growth rates and/or late sexual maturity in females, which favors larger body size for accommodating larger ovaries, and thus, higher reproductive output

High genetic diversity but low population structure in the frog Pseudopaludicola falcipes (Hensel, 1867) (Amphibia, Anura) from the Pampas of South America

Relative to South America’s ecoregions, the temperate grasslands of the Pampas have been poorly studied from a phylogeographic perspective. Based on an intermediate biogeographic setting between subtropical forest (Atlantic Forest) and arid ecosystems (Chaco and Patagonia), Pampean species are expected to show unstable demographic histories due to the Quaternary climatic oscillations. Herein, we investigate the phylogenetic relatedness and phylogeographic history of Pseudopaludicola falcipes, a small and common frog that is widely distributed across the Pampean grasslands. First, we use molecular data to assess if P. falcipes represents a single or multiple, separately evolving cryptic lineages. Because P. falcipes is a small-size species (\u3c20 mm) with extensive coloration and morphological variation, we suspected that it might represent a complex of cryptic species. In addition, we expected strong genetic and geographic structuring within Pseudopaludicola falcipes due to its large geographic distribution, potentially short dis- persal distances, and multiple riverine barriers. We found that P. falcipes is a single evolutionary lineage with poor geographic structuring. Furthermore, current populations of P. falcipes have a large effective population size, maintain ancestral polymorphisms, and have a complex network of gene flow. We conclude that the demographic history of P. falcipes, combined with its ecological attributes and the landscape features of the Pampas, favored a unique combination among anurans of small body size, large population size, high genetic variability, but high cohesiveness of populations over a wide geographic distribution

Are \u3cem\u3eLeptodactylus didymus\u3c/em\u3e and \u3cem\u3eL. mystaceus\u3c/em\u3e Phylogenetically Sibling Species (Amphibia, Anura, Leptodactylidae)?

The Leptodactylus fuscus species group consists of 25 currently recognized species; within this species group and distributed throughout the Amazon Basin, Atlantic Forests, Gran Chaco, and cerrados is the L. mystaceus species complex. This species complex consists of L. didymus, L. elenae, L. mystaceus, L. notoaktites, and L. spixi. Adult morphologies have been used to distinguish these species from each other except for L. didymus and L. mystaceus (Heyer, 1978; Heyer et al., 1996). Leptodactylus didymus and L. mystaceus are morphologically indistinguishable; the species are recognizable only by the characteristics of their advertisement calls: non-pulsed in L. didymus and pulsed in L. mystaceus (Heyer et al., 1996). Traditionally, L. mystaceus and L. didymus have been considered sibling species. The concept of sibling species was originally introduced by Mayr (1942: 151) to describe pairs or groups of morphologically identical or nearly identical species; however, in subsequent work Mayr (1976) interchangeably used the terms sibling and cryptic species to describe morphologically similar species. Mayr (1942: 151) considered sibling species to be important in understanding the full complexity of animal speciation. In order to differentiate these two terms, herein we take a narrow cladistic methodological approach (i.e., dichotomous speciation) by which we restrict the term sibling species to two taxa that share a most recent common ancestor; whereas, the term cryptic (derived from the Greek Kruptos, meaning \u27hidden\u27; Allaby, 1991) species refers to hidden diversity and does not necessarily imply close phylogenetic relationship. Thus, the sibling species pair of L. didymus and L. mystaceus assumes two postulates: (1) the taxa shared a most recent common ancestor not shared with other species in the L. mystaceus species complex and (2) the two taxa could represent a recent speciation event (i.e., not enough time has passed to reach morphological differentiation, although this is not a requisite). Herein, we analyze the genetic diversity among taxa in this species complex to determine if the sibling species L. didymus and L. mystaceus are sister taxa. If the assumptions about sibling species are correct, then we would expect that the two taxa involved would be genetically closer between themselves than with any other closely related species

Phylogenetic analyses of mtDNA sequences reveal three cryptic lineages in the widespread neotropical frog Leptodactylus fuscus (Schneider, 1799) (Anura, Leptodactylidae)

Leptodactylus fuscus is a neotropical frog ranging from Panamá to Argentina, to the east of the Andes mountains, and also inhabiting Margarita, Trinidad, and the Tobago islands. We performed phylogenetic analyses of 12S rRNA, 16S rRNA, tRNA-Leu, and ND1 mitochondrial (mt) DNA sequences from specimens collected across the geographic distribution of L. fuscus to examine two alternative hypotheses: (i) L. fuscus is a single, widely distributed species, or (ii) L. fuscus is a species complex. We tested statistically for geographic association and partitioning of genetic variation among mtDNA clades. The mtDNA data supported the hypothesis of several cryptic species within L. fuscus. Unlinked mtDNA and nuclear markers supported independently the distinctness of a ‘northern’ phylogenetic unit. In addition, the mtDNA data divided the southern populations into two clades that showed no sister relationship to each other, consistent with high differentiation and lack of gene flow among southern populations as suggested by allozyme data. Concordance between mtDNA and allozyme patterns suggests that cryptic speciation has occurred in L. fuscus without morphological or call differentiation. This study illustrates a case in which lineage splitting during the speciation process took place without divergence in reproductive isolation mechanisms (e.g. advertisement call in frogs), contrary to expectations predicted using a biological species framework

Phylogeography of the frog Leptodactylus validus (Amphibia: Anura): Patterns and timing of colonization events in the Lesser Antilles

The frog Leptodactylus validus occurs in northern South America, Trinidad and Tobago, and the southern Lesser Antilles (Grenada and St. Vincent). Mitochondrial DNA sequences were used to perform a nested clade phylogeographic analysis (NCPA), to date colonization events, and to analyze colonization patterns using on a relaxed molecular clock and coalescent simulations. L. validus originated on the mainland and first colonized Trinidad with subsequent independent colonizations of Tobago and the Lesser Antilles from Trinidad. The NCPA suggests a historical vicariant event between populations in Trinidad and Tobago from those in the Lesser Antilles. The colonization of Trinidad occurred 1 million years ago (mya) and the colonization of the Lesser Antillean islands occurred 0.4 mya. The coalescent approach supported the scenario where L. validus dispersed from Trinidad to St. Vincent and from there to Grenada, a dispersal event that could have been mediated by human introduction as recent as 1600 years ago

Accuracy and Precision of Species Trees: Effects of Locus, Individual, and Base Pair Sampling on Inference of Species Trees in Lizards of the Liolaemus darwinii Group (Squamata, Liolaemidae)

Molecular phylogenetics has entered a new era in which species trees are estimated from a collection of gene trees using methods that accommodate their heterogeneity and discordance with the species tree. Empirical evaluation of species trees is necessary to assess the performance (i.e., accuracy and precision) of these methods with real data, which consists of gene genealogies likely shaped by different historical and demographic processes. We analyzed 20 loci for 16 species of the South American lizards of the Liolaemus darwinii species group and reconstructed a species tree with *BEAST, then compared the performance of this method under different sampling strategies of loci, individuals, and sequence lengths. We found an increase in the accuracy and precision of species trees with the number of loci, but for any number of loci, accuracy substantially decreased only when using only one individual per species or 25% of the full sequence length (~147 base-pairs). In addition, locus ´informativeness´ was an important factor in the accuracy/precision of species trees when using a few loci, but it became increasingly irrelevant with additional loci. Our empirical results combined with previous simulation studies suggest that there is an optimal range of sampling effort of loci, individuals, and sequence lengths for a given speciation history and information content of the data. Future studies should be directed towards further assessment of other factors that can impact performance of species trees, including gene flow, locus ´informativeness´, tree shape, missing data, and errors in species delimitation.Fil: Camargo Bentaberry, Arley. University Brigham Young; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Avila, Luciano Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; ArgentinaFil: Morando, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; ArgentinaFil: Sites, Jack W.. University Brigham Young; Estados Unido